Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Mamat, Uwe ^a   Wilke, Kathleen ^a   Bramhill, David ^b,h   Schromm, Andra Beate ^a   Lindner, Buko ^a   Kohl, Thomas Andreas ^a   Corchero, José Luis ^c,d   Villaverde, Antonio ^c,d   Schaffer, Lana ^e   Head, Steven Robert ^e   Souvignier, Chad ^b   Meredith, Timothy Charles ^f   Woodard, Ronald Wesley ^g  

^a RESEARCH CENTER BORSTEL   (Germany)

^b Research Corporation Technologies Inc   (United States)

^c BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^d UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^e SCRIPPS RESEARCH INSTITUTE   (United States)

^f PENNSYLVANIA STATE UNIVERSITY   (United States)

^g UNIVERSITY OF MICHIGAN   (United States)

^h Bramhill Biological Consulting LLC   (United States)

Author keywords

Endotoxic activity; Escherichia coli; Lipid A; Lipopolysaccharide; Recombinant protein; TLR4 MD 2 activation

Indexed keywords

ENDOTOXIN; ESCHERICHIA COLI LIPOPOLYSACCHARIDE; LIPID; LIPID IVA; PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; 3-DEOXY-MANNO-OCT-2-ULOPYRANOSONIC ACID; ABC TRANSPORTER; BACTERIAL PROTEIN; D-ARABINOSE 5-PHOSPHATE ISOMERASE, E COLI; ESCHERICHIA COLI PROTEIN; GLYCOLIPID; ISOMERASE; LIPID A; LIPID A PRECURSORS, BACTERIAL; LIPOPOLYSACCHARIDE; MSBA PROTEIN, BACTERIA; SUGAR ACID;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOPROCESS; CELL ENGINEERING; CONTROLLED STUDY; DETOXIFICATION; DRUG MANUFACTURE; DRUG SAFETY; ESCHERICHIA COLI; NONHUMAN; OUTER MEMBRANE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; CARBOHYDRATE ANALYSIS; GENE DELETION; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLIC ENGINEERING; METABOLISM; MOLECULAR GENETICS; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; REPRODUCIBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; NEGIBACTERIA;

ALDOSE-KETOSE ISOMERASES; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; CARBOHYDRATE SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE DELETION; GLYCOLIPIDS; LIPID A; LIPOPOLYSACCHARIDES; MASS SPECTROMETRY; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SUGAR ACIDS;

EID: 84928044220     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0241-5     Document Type: Article

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