Identification and elimination of metabolic bottlenecks in the quinone modification pathway for enhanced coenzyme Q10 production in Rhodobacter sphaeroides

Metabolic Engineering

Volumn 29, Issue , 2015, Pages 208-216

  Lu, Wenqiang ^a   Ye, Lidan ^a,b   Lv, Xiaomei ^a   Xie, Wenping ^a   Gu, Jiali ^a   Chen, Zhaofeng ^c   Zhu, Yongqiang ^a   Li, Aipeng ^a   Yu, Hongwei ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c Group of Bioengineering   (China)

Author keywords

Bottleneck removal; CoQ10; Fusion expression; Intermediate elimination; Quinone modification pathway; Rhodobacter sphaeroides

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; METABOLISM;

COQ10; FUSION EXPRESSION; INTERMEDIATE ELIMINATION; MODIFICATION PATHWAY; RHODOBACTER SPHAEROIDES;

PRODUCTIVITY;

QUINONE DERIVATIVE; UBIDECARENONE; UBIE QUINONE; UBIH QUINONE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CHORISMATE PYRUVATE LYASE; LYASE; METHYLTRANSFERASE; MIXED FUNCTION OXIDASE; UBIQUINONE;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOSYNTHESIS; CHEMICAL MODIFICATION; EXTRACTIVE FERMENTATION; GENE EXPRESSION; METABOLIC ENGINEERING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHODOBACTER SPHAEROIDES; ANALOGS AND DERIVATIVES; GENETICS; METABOLISM;

RHODOBACTER SPHAEROIDES;

BACTERIAL PROTEINS; METHYLTRANSFERASES; MIXED FUNCTION OXYGENASES; OXO-ACID-LYASES; RHODOBACTER SPHAEROIDES; UBIQUINONE;

EID: 84927582054     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.03.012     Document Type: Article

References (42)

1. Ajikumar P.K., Xiao W.H., Tyo K.E.J., Wang Y., Simeon F., Leonard E., Mucha O., Phon T.H., Pfeifer B., Stephanopoulos G. Isoprenoid pathway optimization for taxol precursor overproduction in Escherichia coli. Science 2010, 330:70-74.
2. Aklujkar M., Beatty J.T. Investigation of Rhodobacter capsulatus PufX interactions in the core complex of the photosynthetic apparatus. Photosynth. Res. 2006, 88:159-171.
3. Anthony J.R., Anthony L.C., Nowroozi F., Kwon G., Newman J.D., Keasling J.D. Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene. Metab. Eng. 2009, 11:13-19.
4. Barz W.P., Vermeglio A., Francia F., Venturoli G., Melandri B.A., Oesterhelt D. Role of the PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 2. PufX is required for efficient ubiquinone/ubiquinol exchange between the reaction center QB site and the cytochrome bc1 complex. Biochemistry 1995, 34:15248-15258.
5. 10 as a potential treatment. J. Bioenerg. Biomembr. 2004, 36:381-386.
6. 10 by overexpressing HMG-CoA reductase and induction with arachidonic acid in Schizosaccharomyces pombe. Appl. Biochem. Biotechnol. 2010, 160:523-531.
7. 10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene. J. Biotechnol. 2009, 144:64-69.
8. 10. Appl. Microbiol. Biotechnol. 2005, 68:9-15.
9. 10 in microbes. Trends Biotechnol. 2007, 25:514-521.
10. 10. Metab. Eng. 2011, 13:733-744.
11. Conrado R.J., Wu G.C., Boock J.T., Xu H., Chen S.Y., Lebar T., Turnsek J., Tomsic N., Avbelj M., Gaber R., Koprivnjak T., Mori J., Glavnik V., Vovk I., Bencina M., Hodnik V., Anderluh G., Dueber J.E., Jerala R., Delisa M.P. DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency. Nucleic Acids Res. 2012, 40:1879-1889.
12. Dahl R.H., Zhang F., Alonso-Gutierrez J., Baidoo E., Batth T.S., Redding-Johanson A.M., Petzold C.J., Mukhopadhyay A., Lee T.S., Adams P.D., Keasling J.D. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 2013, 31:1039-1046.
13. Dhanasekaran M., Ren J. The emerging role of coenzyme Q-10 in aging, neurodegeneration, cardiovascular disease, cancer and diabetes mellitus. Curr. Neurovasc. Res. 2005, 2:447-459.
14. Dueber J.E., Wu G.C., Malmirchegini G.R., Moon T.S., Petzold C.J., Ullal A.V., Prather K.L., Keasling J.D. Synthetic protein scaffolds provide modular control over metabolic flux. Nat. Biotechnol. 2009, 27:753-759.
15. Gorke B., Stulke J. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat. Rev. Microbiol. 2008, 6:613-624.
16. Gulmezian M., Hyman K.R., Marbois B.N., Clarke C.F., Javor G.T. The role of UbiX in Escherichia coli coenzyme Q biosynthesis. Arch. Biochem. Biophys. 2007, 467:144-153.
17. Holden-Dye K., Crouch L.I., Jones M.R. Structure, function and interactions of the PufX protein. Biochim. Biophys. Acta-Bioenerg. 2008, 1777:613-630.
18. 10 Chinese. J. Chem. Eng. 2011, 19:316-326.
19. 10 production in recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 2006, 72:982-985.
20. Kim S.W., Keasling J.D. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol. Bioeng. 2001, 72:408-415.
21. Knoell H.E. Isolation of a soluble enzyme complex comprising the ubiquinone-8 synthesis apparatus from the cytoplasmic membrane of Escherichia coli. Biochem. Biophys. Res. Commun. 1979, 91:919-925.
22. 10 production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens. Biosci. Biotechnol. Biochem. 2010, 74:895-898.
23. Kroll J., Steinle A., Reichelt R., Ewering C., Steinbuchel A. Establishment of a novel anabolism-based addiction system with an artificially introduced mevalonate pathway: complete stabilization of plasmids as universal application in white biotechnology. Metab. Eng. 2009, 11:168-177.
24. Lee M.E., Aswani A., Han A.S., Tomlin C.J., Dueber J.E. Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay. Nucleic Acids Res. 2013, 41:10668-10678.
25. Lim H.N., Lee Y., Hussein R. Fundamental relationship between operon organization and gene expression. Proc. Natl. Acad. Sci. USA 2011, 108:10626-10631.
26. 10 by constitutive overexpression of 3-demethyl ubiquinone-9 3-methyltransferase under tac promoter in Rhodobacter sphaeroides. Biochem. Eng. J. 2013, 72:42-47.
27. 10 by self-regulating the engineered MEP pathway in Rhodobacter sphaeroides. Biotechnol. Bioeng. 2014, 111:761-769.
28. Ma S.M., Garcia D.E., Redding-Johanson A.M., Friedland G.D., Chan R., Batth T.S., Haliburton J.R., Chivian D., Keasling J.D., Petzold C.J., Lee T.S., Chhabra S.R. Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases. Metab. Eng. 2011, 13:588-597.
29. Marbois B., Gin P., Faull K.F., Poon W.W., Lee P.T., Strahan J., Shepherd J.N., Clarke C.F. Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q. J. Biol. Chem. 2005, 280:20231-20238.
30. Martin V.J., Pitera D.J., Withers S.T., Newman J.D., Keasling J.D. Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat. Biotechnol. 2003, 21:796-802.
31. Okada K., Kainou T., Tanaka K., Nakagawa T., Matsuda H., Kawamukai M. Molecular cloning and mutational analysis of the ddsA gene encoding decaprenyl diphosphate synthase from Gluconobacter suboxydans. Eur. J. Biochem. 1998, 255:52-59.
32. Onodera S., Suzuki H., Shimada Y., Kobayashi M., Nozawa T., Wang Z.Y. Overexpression and characterization of the Rhodobacter sphaeroides PufX membrane protein in Escherichia coli. Photochem. Photobiol. 2007, 83:139-144.
33. 10 in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans. Appl. Microbiol. Biotechnol. 2005, 67:192-196.
34. Pitera D.J., Paddon C.J., Newman J.D., Keasling J.D. Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab. Eng. 2007, 9:193-207.
35. Rodríguez-Villalón A., Pérez-Gil J., Rodríguez-Concepción M. Carotenoid accumulation in bacteria with enhanced supply of isoprenoid precursors by upregulation of exogenous or endogenous pathways. J. Biotechnol. 2008, 135:78-84.
36. 3 linker sequence results in enhanced protein expression. Mol. Immunol. 2004, 40:717-722.
37. Wang C., Yoon S.H., Jang H.J., Chung Y.R., Kim J.Y., Choi E.S., Kim S.W. Metabolic engineering of Escherichia coli for alpha-farnesene production. Metab. Eng. 2011, 13:648-655.
38. 10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway. Metab. Eng. 2006, 8:406-416.
39. Zelcbuch L., Antonovsky N., Bar-Even A., Levin-Karp A., Barenholz U., Dayagi M., Liebermeister W., Flamholz A., Noor E., Amram S. Spanning high-dimensional expression space using ribosome-binding site combinatorics. Nucleic Acids Res. 2013, 41:e98.
40. Zhang D.W., Li Z., Wang F.H., Shrestha B., Tian P.F., Tan T.W. Expression of various genes to enhance ubiquinone metabolic pathway in Agrobacterium tumefaciens. Enzyme Microb. Technol. 2007, 41:772-779.
41. Zhang H.T., Javor G.T. Regulation of the isofunctional genes ubiD and ubiX of the ubiquinone biosynthetic pathway of Escherichia coli. FEMS Microbiol. Lett. 2003, 223:67-72.
42. Zhu X., Yuasa M., Okada K., Suzuki K., Nakagawa T., Kawamukai M., Matsuda H. Production of ubiquinone in Escherichia coli by expression of various genes responsible for ubiquinone biosynthesis. J. Ferment. Bioeng. 1995, 79:493-495.