Investigation of Rhodobacter capsulatus PufX interactions in the core complex of the photosynthetic apparatus

Photosynthesis Research

Volumn 88, Issue 2, 2006, Pages 159-171

  Aklujkar, Muktak ^a,b   Beatty, J Thomas ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Bacteriochlorophyll; Light harvesting; Photosynthesis; PufX; Purple bacteria; Reaction centre; Rhodobacter; TOXCAT

Indexed keywords

BACTERIAL PROTEIN; PUFX PROTEIN, RHODOBACTER;

ARTICLE; GENE EXPRESSION REGULATION; LIGHT HARVESTING SYSTEM; METABOLISM; PHOTOSYNTHESIS; PLOIDY; RHODOBACTER CAPSULATUS;

BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; LIGHT-HARVESTING PROTEIN COMPLEXES; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS; PLOIDIES; RHODOBACTER CAPSULATUS;

PROTEOBACTERIA; RHODOBACTER; RHODOBACTER CAPSULATUS;

EID: 33745029738     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11120-006-9047-y     Document Type: Article

References (52)

1. Abresch EC, Axelrod HLA, Beatty JT, Johnson JA, Nechushtai R and Paddock ML (2005) Characterization of a highly purified, fully active, crystallizable RC-LH1-PufX core complex from Rhodobacter sphaeroides. Photosynth Res 86: 61-70
2. Aklujkar M and Beatty JT (2005) The PufX protein of Rhodobacter capsulatus affects the properties of bacteriochlorophyll a and carotenoid pigments of light-harvesting complex 1. Arch Biochem Biophys 443: 21-32
3. Aklujkar M, Harmer AL, Prince RC and Beatty JT (2000) The orf162b sequence of Rhodobacter capsulatus encodes a protein required for optimal levels of photosynthetic pigment-protein complexes. J Bacteriol 182: 5440-5447
4. Aklujkar M, Prince RC and Beatty JT (2005a) The PuhB protein of Rhodobacter capsulatus functions in photosynthetic reaction centre assembly with a secondary effect on light-harvesting complex 1. J Bacteriol 187: 1334-1343
5. Aklujkar M. Prince RC and Beatty JT (2005b) The puhE gene of Rhodobacter capsulatus is needed for optimal transition from aerobic to photosynthetic growth and encodes a putative negative modulator of bacteriochlorophyll production. Arch Biochem Biophys 437: 186-198
6. Alberti M, Burke DE and Hearst JE (1995) Structure and sequence of the photosynthetic gene cluster. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 1083-1106. Kluwer Academic Publishers, Dordrecht, The Netherlands
7. Bahatyrova S, Frese RN, Siebert CA, Olsen JD, van der Werf KO, van Grondelle R, Niederman RA, Bullough PA, Otto C and Hunter CN (2004) The native architecture of a photosynthetic membrane. Nature 430: 1058-1062
8. Barz WP, Francia F, Venturoli G, Melandri BA, Verméglio A and Oesterhelt D (1995a) Role of PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 1. PufX is required for efficient light-driven electron transfer and photophosphorylation under anaerobic conditions. Biochemistry 34: 15235-15247
9. 1 complex. Biochemistry 34: 15248-15258
10. Beatty JT and Gest H (1981) Generation of succinyl-coenzyme A in photosynthetic bacteria. Arch Microbiol 129: 335-340
11. Bibb MJ and Cohen SN (1982) Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol Gen Genet 187: 265-277
12. Comayras F, Jungas C and Lavergne J (2005a) Functional consequences of the organization of the photosynthetic apparatus in Rhodobacter sphaeroides. I. Quinone domains and excitation transfer in chromatophores and reaction center antenna complexes. J Biol Chem 280: 11203-11213
13. - membranes. J Biol Chem 280: 11214-11223
14. Ditta G, Schmidhauser T, Yakobsen E, Lu P, Liang X-W, Finlay DR, Guiney D and Helinski DR (1985) Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid 13: 149-153
15. Drews G and Golecki JR (1995) Structure, molecular organization, and biosynthesis of membranes of purple bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 231-257. Kluwer Academic Publishers, Dordrecht, The Netherlands
16. Farchaus JW, Barz WP, Grünberg H and Oesterhelt D (1992) Studies on the expression of the pufX polypeptide and its requirement for photoheterotrophic growth in Rhodobacter sphaeroides. EMBO J 11: 2779-2788
17. Feick R, van Grondelle R, Rijgersberg CP and Drews G (1980) Fluorescence emission by wild-type and mutant strains of Rhodopseudomonas capsulata. Biochim Biophys Acta 593: 241-253
18. - charge separation in reaction centers of Rhodobacter sphaeroides. Biochemistry 43: 14199-14210
19. Francia F, Wang J, Venturoli G, Melandri BA, Barz WP and Oesterhelt D (1999) The reaction center-LH1 antenna complex of Rhodobacter sphaeroides contains one PufX molecule which is involved in dimerization of this complex. Biochemistry 38: 6834-6845
20. Francia F, Wang J, Zischka H, Venturoli G and Oesterhelt D (2002) Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides. Eur J Biochem 269: 1877-1885
21. Frese RN, Olsen JD, Branvall R, Westerhuis WHJ, Hunter CN and van Grondelle R (2000) The long-range supraorganization of the bacterial photosynthetic unit: a key role for PufX. Proc Natl Acad Sci USA 97: 5197-5202
22. Goldsmith JO, King B and Boxer SG (1996) Mg coordination by amino acid side chains is not required for assembly and function of the special pair in bacterial photosynthetic reaction centers. Biochemistry 35: 2421-2428
23. Jackson WJ, Kiley PJ, Haith CE, Kaplan S and Prince RC (1987) On the role of the light-harvesting B880 in the correct insertion of the reaction center of Rhodobacter capsulatus and Rhodobacter sphaeroides. FEBS Lett 215: 171-174
24. Joliot P, Joliot A and Verméglio A (2005) Fast oxidation of the primary electron acceptor under anaerobic conditions requires the organization of the photosynthetic chain of Rhodobacter sphaeroides in supercomplexes. Biochim Biophys Acta 1706: 204-214
25. Jones MR, Fowler GJS, Gibson LCD, Grief GG, Olsen JD, Crielaard W and Hunter CN (1992) Mutants of Rhodobacter sphaeroides lacking one or more pigment-protein complexes and complementation with reaction-centre, LH1, and LH2 genes. Mol Microbiol 6(9): 1173-1184
26. Jungas C, Ranck J-L, Rigaud J-L, Joliot P and Verméglio A (1999) Supramolecular organization of the photosynthetic apparatus of Rhodobacter sphaeroides. EMBO J 18: 534-542
27. Klug G and Cohen SN (1988) Pleiotropic effects of localized Rhodobacter capsulatus puf operon deletions on production of light-absorbing pigment-protein complexes. J Bacteriol 170: 5814-5821
28. Law CJ, Chen J, Parkes-Loach PS and Loach PA (2003) Interaction of bacteriochlorophyll with the LH1 and PufX polypeptides of photosynthetic bacteria: use of chemically synthesized analogs and covalently attached fluorescent probes. Photosynth Res 75: 193-210
29. LeBlanc H, Lang AS and Beatty JT (1999) Transcript cleavage, attenuation and an internal promoter in the Rhodobacter capsulatus puc operon. J Bacteriol 181: 4955-4960
30. Lilburn TG, Haith CE, Prince RC and Beatty JT (1992) Pleiotropic effects of pufX gene deletion on the structure and function of the photosynthetic apparatus of Rhodobacter capsulatus. Biochim Biophys Acta 1100: 160-170
31. Lilburn TG, Prince RC and Beatty JT (1995) Mutation of the Ser2 codon of the light-harvesting B870 α polypeptide of Rhodobacter capsulatus partially suppresses the pufX phenotype. J Bacteriol 177: 4593-4600
32. McGlynn P, Hunter CN and Jones MR (1994) The Rhodobacter sphaeroides PufX protein is not required for photosynthetic competence in the absence of a light harvesting system. FEBS Lett 349: 349-353
33. Papiz MZ, Prince SM, Hawthornthwaite-Lawless AM, McDermott G, Freer AA, Isaacs NW and Cogdell RJ (1996) A model for the photosynthetic apparatus of purple bacteria. Trends Plant Sci 1: 198-206
34. Parkes -Loach PS, Law CJ, Recchia PA, Kehoe J, Nehrlich S, Chen J and Loach PA (2001) Role of the core region of the PufX protein in inhibition of reconstitution of the core light-harvesting complexes of Rhodobacter sphaeroides and Rhodobacter capsulatus. Biochemistry 40: 5593-5601
35. Pugh RJ, McGlynn P, Jones MR and Hunter CN (1998) The LH1-RC core complex of Rhodobacter sphaeroides: interaction between components, time-dependent assembly, and topology of the PufX protein. Biochim Biophys Acta 1366: 301-316
36. Qian P, Hunter CN and Bullough PA (2005) The 8.5 Å projection structure of the core RC-LH1-PufX dimer of Rhodobacter sphaeroides. J Mol Biol 349: 948-960
37. Recchia PA, Davis CM, Lilburn TG, Beatty JT, Parkes-Loach PS, Hunter CN and Loach PA (1998) Isolation of the PufX protein from Rhodobacter capsulatus and Rhodobacter sphaeroides: evidence for its interaction with the α-polypeptide of the core light harvesting complex. Biochemistry 37: 11055-11063
38. Richter P and Drews G (1991) Incorporation of light-harvesting complex I α and β polypeptides into the intracytoplasmic membrane of Rhodobacter capsulatus. J Bacteriol 173: 5336-5345
39. Russ WP and Engelman DM (1999) TOXCAT: a measure of transmembrane helix association in a biological membrane. Proc Natl Acad Sci USA 96: 863-868
40. Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
41. Scheuring S, Busselez J and Lévy D (2005) Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ AFM. J Biol Chem 280: 1426-1431
42. Scheuring S, Francia F, Busselez J, Melandri BA, Rigaud J-L and Lévy D (2004) Structural role of PufX in the dimerization of the photosynthetic core complex of Rhodobacter sphaeroides. J Biol Chem 279: 3620-3626
43. Scolnik PA and Marrs BL (1987) Genetic research with photosynthetic bacteria. Annu Rev of Microbiol 41: 703-726
44. Siebert CA, Qian P, Fotiadis D, Engel A, Hunter CN and Bullough PA (2004) Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX. EMBO J 23: 690-700
45. Solioz M and Marrs B (1977) The gene transfer agent of Rhodopseudomonas capsulata. Arch Biochem Biophys 181: 300-307
46. Tsukatani Y, Matsuura K, Masuda S, Shimada K, Hiraishi A and Nagashima KVP (2004) Phylogenetic distribution of unusual triheme to tetraheme cytochrome subunit in the reaction center complex of purple photosynthetic bacteria. Photosynth Res 79: 83-91
47. Waidner LA and Kirchman DL (2005) Aerobic anoxygenic photosynthesis genes and operons in uncultured bacteria in the Delaware River. Environ Microbiol 7: 1896-1908
48. Westerhuis WHJ, Farchaus JW and Niederman RA (1993) Altered spectral properties of the B875 light-harvesting pigment-protein complex in a Rhodobacter sphaeroides mutant lacking pufX. Photochem Photobiol 58: 460-463
49. Young CS, Reyes RC and Beatty JT (1998) Genetic complementation and kinetic analyses of Rhodobacter capsulatus ORF1696 mutants indicate that the ORF1696 protein enhances assembly of the light-harvesting I complex. J Bacteriol 180: 1759-1765
50. Youvan DC, Ismail S and Bylina EJ (1985) Chromosomal deletion and plasmid complementation of the photosynthetic reaction center and light harvesting genes from Rhodopseudomonas capsulata. Gene 38: 19-30
51. Yutin N and Béjà O (2005) Putative novel photosynthetic reaction centre organizations in marine aerobic anoxygenic photosynthetic bacteria: insights from metagenomics and environmental genomics. Environ Microbiol 7: 2027-2033
52. Zilsel J, Lilburn T and Beatty JT (1989) Formation of functional inter-species hybrid photosynthetic complexes in Rhodobacter capsulatus. FEBS Lett 253: 247-252