A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates

Biotechnology and Bioengineering

Volumn 112, Issue 5, 2015, Pages 867-878

  Buntru, Matthias ^a   Vogel, Simon ^a   Stoff, Katrin ^a   Spiegel, Holger ^a   Schillberg, Stefan ^a  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

BY 2 cell lysate; Cell free protein synthesis; Coupled transcription translation; High throughput screening; Protein expression

Indexed keywords

BIOLOGICAL MEMBRANES; BIOSYNTHESIS; CELL MEMBRANES; CELLS; COVALENT BONDS; CYTOLOGY; GLUCOSE OXIDASE; GLUCOSE SENSORS; RECOMBINANT PROTEINS; THROUGHPUT; TOBACCO; TRANSCRIPTION;

BY-2 CELL; CELL FREE PROTEIN SYNTHESIS; ENHANCED YELLOW FLUORESCENT PROTEINS; FRACTIONAL FACTORIAL DESIGNS; GLUCOSE OXIDASES (GOX); HIGH THROUGHPUT SCREENING; PROTEIN EXPRESSIONS; RESPONSE SURFACE MODELS;

PROTEINS;

CYTOTOXIC FACTOR; FIREFLY LUCIFERASE; GLUCOSE OXIDASE; GLYCOPEPTIDASE; GLYCOPROTEIN; GROWTH FACTOR; MEMBRANE PROTEIN; YELLOW FLUORESCENT PROTEIN; ANTIBODY; BACTERIAL PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; CELL FREE SYSTEM; CELL LYSATE; CONTROLLED STUDY; DISULFIDE BOND; ENDOPLASMIC RETICULUM; FACTORIAL DESIGN; GLYCOSYLATION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MICROSOME; NONHUMAN; PLANT CELL; PLASMID; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RESPONSE SURFACE METHOD; TOBACCO; ANIMAL; BIOTECHNOLOGY; CELL CULTURE; CYTOLOGY; GENETIC TRANSCRIPTION; GENETICS; HUMAN; METABOLISM; PROCEDURES;

NICOTIANA TABACUM;

ANIMALS; ANTIBODIES; BACTERIAL PROTEINS; BIOTECHNOLOGY; CELL-FREE SYSTEM; CELLS, CULTURED; GLYCOSYLATION; HUMANS; LUCIFERASES, FIREFLY; LUMINESCENT PROTEINS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; TOBACCO; TRANSCRIPTION, GENETIC;

EID: 84925598713     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25502     Document Type: Article

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