Tobacco BY-2 cell-free lysate: An alternative and highly-productive plant-based in vitro translation system

BMC Biotechnology

Volumn 14, Issue , 2014, Pages

  Buntru, Matthias ^a   Vogel, Simon ^a   Spiegel, Holger ^a   Schillberg, Stefan ^a  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

Cell free protein synthesis; In vitro translation; Nicotiana tabacum cv. BY 2; Protein expression; Wheat germ extract

Indexed keywords

DIALYSIS; PLANTS (BOTANY); TOBACCO;

CELL FREE PROTEIN SYNTHESIS; IN-VITRO; NICOTIANA TABACUM; PROTEIN EXPRESSIONS; WHEAT GERM EXTRACTS;

PROTEINS;

FISH OIL; LUCIFERASE; RENILLA LUCIFERIN 2 MONOOXYGENASE; RNA 18S; YELLOW FLUORESCENT PROTEIN; 3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; BACTERIAL PROTEIN; MESSENGER RNA; PHOTOPROTEIN; RECOMBINANT PROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; CELL FREE SYSTEM; CELL LYSATE; CELL SUSPENSION; CONTROLLED STUDY; INTERNAL RIBOSOME ENTRY SITE; ISOTOPE LABELING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RENILLA RENIFORMIS; RIBOSOME SUBUNIT; TOBACCO; TRANSLATION INITIATION; WHEAT GERM; BIOSYNTHESIS; CYTOLOGY; DIALYSIS; GENETICS; GERM CELL; METABOLISM; PROTOPLAST; WHEAT;

EUKARYOTA; NICOTIANA TABACUM; ORYCTOLAGUS CUNICULUS; PROKARYOTA; TRITICUM AESTIVUM;

3' UNTRANSLATED REGIONS; 5' UNTRANSLATED REGIONS; BACTERIAL PROTEINS; CELL-FREE SYSTEM; DIALYSIS; GERM CELLS; LUCIFERASES; LUMINESCENT PROTEINS; PROTEIN BIOSYNTHESIS; PROTOPLASTS; RECOMBINANT PROTEINS; RNA, MESSENGER; TOBACCO; TRITICUM;

EID: 84900311214     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-37     Document Type: Article

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