Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ

European Journal of Biochemistry

Volumn 270, Issue 23, 2003, Pages 4780-4786

  Kawasaki, Takayasu ^a   Gouda, Mudeppa D ^a   Sawasaki, Tatsuya ^a   Takai, Kazuyuki ^a   Endo, Yaeta ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Biotinylation; Cell free synthesis; Disulfide; scFv; Wheat germ

Indexed keywords

AGAROSE; ANTIBODY; DISULFIDE; DITHIOTHREITOL; ISOMERASE; MESSENGER RNA; POLYPEPTIDE; STREPTAVIDIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; BIOTINYLATION; GEL FILTRATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SYNTHESIS; RNA TRANSLATION; SOLUBILITY; TRANSLATION INITIATION; WHEAT GERM;

AMINO ACID SEQUENCE; ANTIBODIES; BASE SEQUENCE; BIOTINYLATION; CELL-FREE SYSTEM; DISULFIDES; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PLANT EXTRACTS; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEINS; RECOMBINANT PROTEINS; TRITICUM;

TRITICUM AESTIVUM;

EID: 0345256409     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03880.x     Document Type: Article

References (16)

1. Madin, K., Sawasaki, T., Ogasawara, T. & Endo, Y. (2000) A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc. Natl Acad. Sci. USA 97, 559-564.
2. Sawasaki, T., Hasegawa, Y., Tsuchimochi, M., Kamura, N., Ogasawara, T., Kuroita, T. & Endo, Y. (2002) A bilayer cell-free protein synthesis system for high-throughput screening of gene products. FEBS Lett. 514, 102-105.
3. Sawasaki, T., Ogasawara, T., Morishita, R. & Endo, Y. (2002) A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl Acad. Sci. USA 99, 14652-14657.
4. Hillson, D.A., Lambert, N. & Freedman, R.B. (1984) Formation and isomerization of disulfide bonds in proteins: protein disulfide-isomerase. Methods Enzymol. 107, 281-294.
5. Gething, M.J. & Sambrook, J. (1992) Protein folding in the cell. Nature 355, 33-45.
6. Anand, N.N., Mandel, S., MacKenzie, C.R., Sadowska, J., Sigurskjold, B., Young, N.M., Bundle, D.R. & Narang, S.A. (1991) Bacterial expression and secretion of various single-chain Fv genes encoding proteins specific for a Salmonella serotype B O-antigen. J. Biol. Chem. 266, 21874-21879.
7. Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S., Lee, T., Pope, S.H., Riordan, G.S. & Whitlow, M. (1988) Single-chain antigen-binding proteins. Science 242, 423-426.
8. Zdanov, A., Li, Y., Bundle, D.R., Deng, S., MacKenzie, C.R., Narang, S.A., Young, N.M. & Cygler, M. (1994) Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-Å resolution. Proc. Natl Acad. Sci. USA 91, 6423-6427.
9. Schatz, P.J. (1993) Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology 11, 1138-1143.
10. Altman, E. & Bundle, D.R. (1994) Polysaccharide affinity columns for purification of lipopolysaccharide-specific murine monoclonal antibodies. Methods Enzymol. 117, 243-253.
11. Jurado, P., Ritz, D., Beckwith, J., Lorenzo, V. & Fernandez, L.A. (2002) Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 320, 1-10.
12. Nygren, H., Werthen, M. & Stenberg, M. (1987) Kinetics of antibody binding to solid-phase-immobilised antigen. J. Immunol. Methods 101, 63-71.
13. Pavlickova, P., Knappik, A., Kambhampati, D., Ortigao, F. & Hug, H. (2003) Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer. Biotechniques 34, 124-130.
14. Netzer, W.J. & Hartle, F.U. (1997) Recombination of protein domains facilitated by co-translational folding in eukaryotes. Nature 388, 343-349.
15. Ryabova, L.A., Desplancq, D., Spirin, A.S. & Pluckthun, A. (1997) Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones. Nat. Biotechnol. 15, 79-84.
16. Jiang, X., Ookubo, Y., Fujii, I., Nakano, H. & Yamane, T. (2002) Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system. FEBS Lett. 514, 290-294.