Ultrastructural and biochemical alterations during browning of pigeon orchid (Dendrobium crumenatum Swartz) callus

Plant Cell, Tissue and Organ Culture

Volumn 121, Issue 1, 2015, Pages 53-69

  Kaewubon, Paveena ^a   Hutadilok Towatana, Nongporn ^a   Teixeira da Silva, Jaime A ^b   Meesawat, Upatham ^a  

^a PRINCE OF SONGKLA UNIVERSITY   (Thailand)

^b NONE   (Japan)

Author keywords

Browning; Phenolic content; Pigeon orchid; Polyphenol oxidase; Swollen chloroplast; Tannin

Indexed keywords

BIOCHEMISTRY; CELL DEATH; FLAVONOIDS; FORESTRY; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MITOCHONDRIA; SCANNING ELECTRON MICROSCOPY; TANNINS;

BROWNING; PHENOLIC CONTENT; PIGEON ORCHID; POLYPHENOL OXIDASE; SWOLLEN CHLOROPLAST;

BIODIVERSITY;

EID: 84925537884     PISSN: 01676857     EISSN: 15735044     Source Type: Journal    
DOI: 10.1007/s11240-014-0678-y     Document Type: Article

References (119)

1. Adelberg JW, Desamero NV, Hale SA, Young RE (1997) Long-term nutrient and water utilization during micropropagation of Cattleya on a liquid/membrane system. Plant Cell Tissue Org Cult 48:1–7
2. Ahmad I, Hussain T, Ashraf I, Nafees M, Maryam RM, Iqbal M (2013) Lethal effects of secondary metabolites on plant tissue culture. Am-Eurasian J Agric Environ Sci 13:539–547
3. Amirjani MR (2010) Effect of NaCl on some physiological parameters of rice. Eur J Biol Sci 3(1):6–16
4. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
5. Appezzato-da-Glória B, Machado SR (2004) Ultrastructural analysis of in vitro direct and indirect organogenesis. Revista Brasil Bot 27(3):429–437
6. Arnaldos TL, Muñoz R, Ferrer MA, Calderón AA (2001) Changes in phenol content during strawberry (Fragaria × ananassa, cv. Chandler) callus culture. Physiol Plant 113:315–322
7. Bairu MW, Aremu AO, Van Staden J (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173
8. Begum AA, Tamaki M, Tahara M, Kako S (1994) Somatic embryogenesis in Cymbidium through in vitro culture of inner tissue of protocorm-like bodies. J Jpn Soc Hortic Sci 63(2):419–427
9. Belarmino MM, Mii M (2000) Agrobacterium-mediated genetic transformation of a Phalaenopsis orchid. Plant Cell Rep 19:435–442
10. Bennici A, Tani C (2009) Ultrastructural effects of salinity in Nicotiana bigelovii var. bigelovii callus cells and Allium cepa roots. Caryologia 62(2):124–133
11. Benson EE (2000) In vitro plant recalcitrance: an introduction. In Vitro Cell Dev Biol Plant 36:141–148
12. Bewick V, Cheek L, Ball J (2004) Statistics review 9: one-way analysis of variance. Crit Care 8(2):130–136
13. Bi YH, Chen WL, Zhang WN, Zhou Q, Yun LJ, Xing D (2009) Production of reactive oxygen species, impairment of photosynthetic function and dynamic changes in mitochondria are early events in cadmium-induced cell death in Arabidopsis thaliana. Biol Cell 101:629–643
14. Bian W, Barsan C, Egea I, Purgatto E, Chervin C, Zouine M, Latché A, Bouzayen M, Pech JC (2011) Metabolic and molecular events occurring during chromoplast biogenesis. J Bot. Article ID 289859, 13 pp
15. Bobák M, Šamaj J, Pretová A, Blehová A, Hlinková E, Ovecka M, Hlavacka A, Kutarnová Z (2004) The histological analysis of indirect somatic embryogenesis on Drosera spathulata Labill. Acta Physiol Plant 26(3):353–361
16. Boguszewska D, Zagdańska B (2012) ROS as signaling molecules and enzymes of plant response to unfavorable environmental conditions. In: Lushchak W, Semchyshyn HM (eds) Oxidative stress-molecular mechanisms and biological effects. InTech, Rijeka, pp 341–362
17. Bréhélin C, Kessler F, van Wijk KJ (2007) Plastoglobules: versatile lipoprotein particles in plastids. Trends Plant Sci 12:260–266
18. Brillouet JM, Romieu C, Schoefs B, Solymosi K, Cheynier V, Fulcrand H, Verdeil JL, Conéjéro G (2013) The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot 112:1003–1014
19. Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tissue Org Cult 64:145–157
20. Chang C, Chang WC (1998) Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep 17:251–255
21. Chapman KD, Dyer JM, Mullen RT (2012) Biogenesis and functions of lipid droplets in plants. J Lipid Res 53(2):215–226
22. Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520
23. Dai J (1993) Postharvest leaf blackening in Protea neriifolia R. Br. Ph.D. Dissertation, University of Hawaii
24. de Oliveira Ribeiro L, Paiva LV, Pádua MS, Santos BR, Alves E, Stein VC (2012) Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã. Acta Sci Agron 34:423–429
25. de Pinto MC, de Gara L (2004) Changes in the ascorbate metabolism of apoplastic and symplastic spaces are associated with cell differentiation. J Exp Bot 55(408):2559–2569
26. Debnath M, Malik CP, Bisen PS (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Curr Pharm Biotechnol 7:33–49
27. Dubravina GA, Zaytseva SM, Zagoskina NV (2005) Changes in formation and localization of phenolic compounds in the tissues of European and Canadian yew during dedifferentiation in vitro. Russ J Plant Physiol 52(5):672–678
28. El-Tayeb MA (2005) Response of barley gains to the interactive effect of salinity and salicylic acid. Plant Growth Regul 45:215–225
29. Feder N, O’Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142
30. Forters AM, Pais MS (2000) Organogenesis from internode-derived nodules of Humulus lupulus var. Nugget (Cannabinaceae): histological studies and changes in the starch content. Am J Bot 87(7):971–979
31. Fu Z, Xu P, He S, Teixeira da Silva JA, Tanaka M (2011) Dynamic changes in enzyme activities and phenolic content during in vitro rooting of tree peony (Paeonia suffruticosa Andr) plantlets. Maejo Int J Sci Technol 5(2):252–265
32. Gacche RN, Shete AM, Dhole NA, Ghole VS (2006) Reversible inhibition of polyphenol oxidase from apple using L-cysteine. Indian J Chem Technol 13:459–463
33. George EF (2008) Plant tissue culture procedure - background. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, vol 1, 3rd edn., The BackgroundSpringer, Dordrecht, pp 1–28
34. Ghozlène I, Mohammed-Réda D, Nedjoud G, Houria B, Ali C (2013) Oxidative stress, chlorophyll content and ROS production and localization in Triticum durum seed. Ann Biol Res 4(5):11–15
35. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
36. Gunawardena AHLAN, Sault K, Donnelly P, Greenwood JS, Dengler NG (2005) Programmed cell death and leaf morphogenesis in Monstera obliqua (Araceae). Planta 221:607–618
37. Gunawardena AHLAN, Greenwood JS, Dengler NG (2007) Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot 94(7):1116–1128
38. Hazubska-Przybył T, Bojarczuk K, Guzicka M (2008) Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika. Dendrobiology 60:19–28
39. He Y, Guo X, Lu R, Niu B, Pasapula V, Hou P, Cai F, Xu Y, Chen F (2009) Changes in morphology and biochemical indices in browning callus from Jatropha curcas hypocotyls. Plant Cell Tissue Org Cult 98:11–17
40. Hofius D, Schultz-Larsen T, Joensen J, Tsitsigiannis DI, Petersen NHT, Mattsson O, Jørgensen LB, Jones JDG, Mundy J, Petersen M (2009) Autophagic components contribute to hypersensitive cell death in Arabidopsis. Cell 137:773–783
41. Hossain MM, Kant R, Van PT, Winarto B, Zeng S, Teixeira da Silva JA (2013) The application of biotechnology to orchids. Crit Rev Plant Sci 32(2):69–139
42. Hu J, Baker A, Bartel B, Linka N, Mullen RT, Reumann S, Zolman BK (2012) Plant peroxisomes: biogenesis and function. Plant Cell 24:2279–2303
43. Huang LC, Lee YL, Huang BL, Kuo CI, Shaw JF (2002) High polyphenol oxidase activity and low titratable acidity in browning bamboo tissue culture. In Vitro Cell Dev Biol Plant 38:358–365
44. Jones AMP, Saxena PK (2013) Inhibition of phenylpropanoid biosynthesis in Artemisia annua L.: a novel approach to reduce oxidative browning in plant tissue culture. PLoS ONE 8(10):e76802
45. Khosravi AR, Kadir MA, Kazemin SB, Zaman FQ, de Silva AE (2008) Establishment of a plant regeneration system from callus of Dendrobium cv. Serdang beauty. Afr J Biotechnol 7(22):4093–4099
46. Kratsch HA, Wise RR (2000) The ultrastructure of chilling stress. Plant, Cell Environ 23:337–350
47. Laukkanen H, Rautiainen L, Taulavuori E, Hohtola A (2000) Changes in cellular structures and enzymatic activities during browning of Scots pine callus derived from mature buds. Tree Physiol 20:467–475
48. Lee SH, Singh AP, Chung GC, Kim YS, Kong IB (2002) Chilling root temperature causes rapid ultrastructural changes in cortical cells of cucumber (Cucumis sativus L.) root tips. J Exp Bot 53:2225–2237
49. Leng P, Su S, Wei F, Yu F, Duan Y (2009) Correlation between browning, total phenolic content, polyphenol oxidase and several antioxidation enzymes during pistachio tissue culture. Acta Hort 829:127–132
50. Leong TM, Wee YC (2013) Observations of pollination in the pigeon orchid, Dendrobium crumenatum Swartz (Orchidaceae) in Singapore. Nat Singap 6:91–96
51. Ling ACK, Yap CP, Shaib JM, Vilasini P (2007) Induction and morphogenesis of Phalaenopsis callus. J Trop Agric Food Sci 35(1):147–152
52. Liu FP, Chen LX (2010) Redox dynamics during embryogenic callus induction of Phalaenopsis spp. J Wuhan Bot Res 28(6):737–743
53. Marín-Méndez W, Sanchéz-Chacón E, Gatica-Arias AM, Ramírez-Fonseca P, Freer-Bustamante E, Valdez-Melara M (2009) Ultrastructure and histology of organogenesis induced from shoot tips of maize (Zea mays, Poaceae). Rev Biol Trop 57(1):129–139
54. Matile PH, Moor H (1968) Vacuolation: origin and development of the lysosomal apparatus in root-tip cells. Planta 80:159–175
55. Mauseth JD (2014) Botany: an introduction to plant biology, 5th edn. Jones & Bartlett Learning, Burlington
56. Mayer AM (2006) Polyphenol oxidases in plants and fungi: going places? A review. Phytochemistry 67:2318–2331
57. Meesawat U, Kanchanapoom K (2002) In vitro plant regeneration through embryogenesis and organogenesis from callus culture of pigeon orchid (Dendrobium crumenatum Sw.). Thammasart Int J Sci Tech 7(2):9–17
58. Meesawat U, Kanchanapoom K (2007) Understanding the flowering behavior of pigeon orchid (Dendrobium crumenatum Swartz). Orchid Sci Biotech 1:6–14
59. Misra P, Toppo DD, Gupta N, Chakrabarty D, Tuli R (2010) Effect of antioxidants and associate changes in antioxidant enzymes in controlling browning and necrosis of proliferating shoots of elite Jatropha curcas L. Biomass Bioenerg 34:1861–1869
60. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7(9):405–410
61. Mondal T, Aditya S, Banerjee N (2013) In vitro axillary shoot regeneration and direct protocorm-like body induction from axenic shoot tips of Doritis pulcherrima Lindl. Plant Tissue Cult Biotechnol 23(2):251–261
62. Mueller WC, Greenwood AD (1978) The ultrastructure of phenolic-storing cells fixed with caffeine. J Exp Bot 29(110):757–764
63. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
64. 2 on the yield and chemical composition of mung bean (Vigna radiata). J Stress Physiol Biochem 10(1):316–325
65. Naz S, Ali A, Iqbal J (2008) Phenolic content in vitro cultures of chickpea (Cicer arietinum L) during callogenesis and organogenesis. Pak J Bot 40(6):2525–2539
66. North JJ, Ndakidemi PA, Laubscher CP (2012) Effects of antioxidants, plant growth regulators and wounding on phenolic compound excretion during micropropagation of Strelitzia reginae. Int J Phys Sci 7(4):638–646
67. Oren-Shamir M (2009) Does anthocyanin degradation play a significant role in determining pigment concentration in plants? Plant Sci 177:310–316
68. Palama TL, Menard P, Fock I, Choi YH, Bourdon E, Govinden-Soulange J, Bahut M, Payet B, Verpoorte R, Kodja H (2010) Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biol 10(82):1–18
69. Park SY, Shin KS, Paek KY (2006) Increased ethylene and decreased phenolic compound stimulate somatic embryo regeneration in leaf thin section cultures of Doritaenopsis hybrid. J Plant Biol 49(5):358–363
70. Peng H, Zhang J (2009) Plant genomic DNA methylation in response to stresses: potential applications and challenges in plant breeding. Prog Nat Sci 19:1037–1045
71. Pihakaski-Maunsbach K, Brauner Nygaard K, Jensen KH, Rasmussen O (1993) Cellular changes in early development of regenerating thin cell layer-explants of rapeseed analyzed by light and electron microscopy. Physiol Plant 87:167–176
72. Pitzschke A, Djamei A, Bitton F, Hirt H (2009) A major role of the MEKK1–MKK1/2–MPK4 pathway in ROS signalling. Mol Plant 2(1):120–137
73. Ramel F, Birtic S, Cuiné S, Triantaphylidès C, Ravanat JL, Havaux M (2012) Chemical quenching of singlet oxygen by carotenoids in plants. Plant Physiol 158:1267–1278
74. Reynolds S (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
75. Ruzin S (1999) Plant microtechnique and microscopy. Oxford University Press, New York
76. Sandalio LM, Rodríguez-Serrano M, Romero-Puertas MC, del Río LA (2013) Role of peroxisomes as a source of reactive oxygen species (ROS) signaling molecules. In: del Río LA (ed) Peroxisomes and their key role in cellular signaling and metabolism. Springer, Dordrecht, pp 231–255
77. Santiago LJM, Louro RP, de Oliveira DE (2000) Compartmentation of phenolic compounds and phenylalanine ammonia-lyase in leaves of Phyllanthus tenellus Roxb. and their induction by copper sulphate. Ann Bot 86:1023–1032
78. Sapers GM, Hicks KB, Miller RL (2002) Antibrowning agents. In: Brane AL, Davidson PM, Salminen S, Thorngate JH III (eds) Food additives, 2nd edn. Marcel Dekker, New York, pp 543–561
79. Sevengor S, Yasar F, Kusvuran S, Ellialtioglu S (2011) The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. Afr J Agric Res 6(21):4920–4924
80. Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. Article ID 217037, 26 pp
81. Shi Q (2011) Studies on preventive technique and browning mechanism in the tissue culture of Cymbidium longibracteatum ‘Cui-xian’ flower stalks. M.Sc. Dissertation, Sichuan Agricultural University
82. Singh A, Prasad R (2009) Salt stress effects growth and cell wall bound enzymes in Arachis hypogaea L. seedlings. Int J Integr. Biol 7(2):117–123
83. Speranza A, Ferri P, Battistelli M, Falcieri E, Crinelli R, Scoccianti V (2007) Both trivalent and hexavalent chromium strongly alter in vitro germination and ultrastructure of kiwifruit pollen. Chemosphere 66(7):1165–1174
84. Stefanowska M, Kuraś M, Kacperska A (2002) Low temperature-induced modifications in cell ultrastructure and localization of phenolics in water oilseed rape (Brassica napus L. var. oleifera L.) leaves. Ann Bot 90:637–645
85. Stein VC, Paiva R, Vargas DP, Soares FP, Alves E, Nogueira GF (2010) Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn Rev Árvore 34:789–796
86. Steinmacher DA, Guerra MP, Saare-Surminski K, Lieberei R (2011) A temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis. Ann Bot 108:1463–1475
87. Stewart SL, Zettler LW (2002) Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H. quinquiseta, H. macroceratitis) from Florida. Aquat Bot 72:25–35
88. Sunkar R (2010) Plant stress tolerance methods and protocols. Humana Press, New York
89. Tang W, Newton RJ (2004) Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill.). Plant Sci 167:621–628
90. Teixeira da Silva JA (2012) Callus, calluses or calli: multiple plurals? Asian Australasian J Plant Sci Biotechnol 6(Special issue 1):125–126
91. Teixeira da Silva JA, Tanaka M (2006) Multiple regeneration pathways via thin cell layers in hybrid Cymbidium (Orchidaceae). J Plant Growth Reg 25(3):203–210
92. Teixeira da Silva JA, Dobránszki J (2013) How timing of sampling can affect the outcome of the quantitative assessment of plant organogenesis. Sci Hortic 159:59–66
93. Terzi R, Saruhan Güler N, Kutlu Çalişkan N, Kadioğlu A (2013) Lignification response for rolled leaves of Ctenanthe setosa under long-term drought stress. Turk J Biol 37:614–619
94. Toivonen PMA, Brummell DA (2008) Biochemical bases of appearance and texture changes in fresh-cut fruit and vegetables. Postharvest Biol Technol 48:1–14
95. Tokuhara K, Mill M (2001) Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cell Dev Biol Plant 37:457–461
96. Tripathy BC, Oelmüller R (2012) Reactive oxygen species generation and signaling in plants. Plant Signal Behav 7(12):1621–1633
97. Vacin E, Went F (1949) Some pH changes in nutrient solution. Bot Gaz 110:605–613
98. Verdeil JL, Alemanno L, Niemenak N, Tranbarger TJ (2007) Pluripotent versus totipotent plant stem cells: dependence versus autonomy? Trends Plant Sci 12(6):245–252
99. Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223
100. Wang W, Messing J (2012) Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed). BMC Plant Biol 12(5):1–14
101. Wang JB, Wang XS, Jin ZQ (2010) Enzymatic browning of postharvest litchi: a review. Acta Hortic 863:613–618
102. Wang XD, Nolan KE, Irwanto RR, Sheahan MB, Rose RJ (2011) Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Ann Bot 107:599–609
103. Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K (2012) Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J 5(2):60–67
104. Wi SG, Chung BY, Kim JH, Baek MH, Yan DH, Lee JW, Kim JS (2005) Ultrastructural changes of cell organelles in Arabidopsis stems after gamma irradiation. J Plant Biol 48(2):195–200
105. Wu Z, Chen LJ, Long YJ (2009) Analysis of ultrastructure and reactive oxygen species of hyperhydric garlic (Allium sativum L.) shoots. In Vitro Cell Dev Biol Plant 45:483–490
106. Xu CJ, Li L (2006) Changes of total phenol content and the activities of PPO, POD and PAL during the browning in Phalaenopsis explant in vitro. Acta Hortic Sin 33(3):671–674 (in Chinese with English abstract)
107. Xu CJ, Li L, Li H, Zhang MG (2005) Preliminary studies on the elements of browning and the changes in cellular texture of leaf explant browning in Phalaenopsis. Acta Hortic Sin 32(6):1111–1113 (in Chinese with English abstract)
108. Xu CJ, Tan RF, Chen DY, Lai YY, Li L (2010) Ultrastructure and distribution of phenol in Phalaenopsis browning leaf explants. North Hortic 21:90–92 (in Chinese with English abstract)
109. Yamauchi N, Funamoto Y, Shigyo M (2004) Peroxidase-mediated chlorophyll degradation in horticultural crops. Phytochem Rev 3:221–228
110. Yang L, Han R, Sun Y (2013) Effects of exogenous nitric oxide on wheat exposed to enhanced ultraviolet-B radiation. Am J Plant Sci 4:1285–1290
111. Yin F, Ge H, Peng K, Zhao L, Zhou Y, Li Q (2006) The influence of phenols on tissue browning of Phalaenopsis. Acta Hortic Sin 33(5):1137–1140 (in Chinese with English abstract)
112. Yingsanga P, Srilaonga V, Kanlayanarat S, Noichindab S, McGlassonc WB (2008) Relationship between browning and related enzymes (PAL, PPO and POD) in rambutan fruit (Nephelium lappaceum Linn.) cvs. Rongrien and See-Chompoo. Postharvest Biol Technol 50:164–168
113. Yoruk R, Marshall MR (2003) Physicochemical properties and function of polyphenol oxidase: a review. J Food Biochem 27:361–422
114. Yoshinaga K, Arimura SI, Niwa Y, Tsutsumi N, Uchimiya H, Kawai-Yamada M (2005) Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana. Ann Bot 96:337–342
115. Zenkteler E, Kwaśna H (2007) Pre-treatment of Dryopteris cristata (L.). A Gray rhizome as a method of elimination of contaminants and explant browning micropropagation. Biodivers Res Conserv 5(8):81–86
116. Zhang X, Ervin EH, Schmidt RE (2005) The role of leaf pigment and antioxidant levels in UV-B resistance of dark- and light-green Kentucky bluegrass cultivars. J Am Soc Hortic Sci 130(6):836–841
117. Zhao P, Wu F, Feng FS, Wang WJ (2008) Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cell Dev Biol Plant 44:178–185
118. Zhao Y, Yang SH, Ge WY, Li QX, Chen HX, Ge H (2010) The metabolism of phenolics and reactive oxygen species in relation to the explant browning differences among the varieties of Phalaenopsis during the tissue culture. Acta Hortic Sin 37:963–970 (in Chinese with English abstract)
119. Zhou W, Tan R, Xu C, Lai Y, Chen D, Li L (2009) Gibberellic acid inhibits browning, enzyme activity and gene expression of phenylalanine ammonia-lyase in Phalaenopsis leaf explants. Genes Genom Genomics 3:68–71