Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host

Applied Microbiology and Biotechnology

Volumn 99, Issue 6, 2015, Pages 2727-2739

  Celińska, Ewelina ^a   Białas, Wojciech ^a   Borkowska, Monika ^a   Grajek, Włodzimierz ^a  

^a POZNA UNIVERSITY OF LIFE SCIENCES   (Poland)

Author keywords

Amylase; Granular starch; Recombinant protein expression; Rice weevil; Yarrowia lipolytica

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANIMALS; CLONING; ENZYMES; PURIFICATION; RECOMBINANT PROTEINS; STARCH;

ALPHA-AMYLASE ACTIVITY; AMMONIUM SULFATE PRECIPITATION; GRANULAR STARCHES; PURIFICATION FACTORS; PURIFICATION PROCEDURES; RECOMBINANT PROTEIN EXPRESSION; RICE WEEVIL; YARROWIA LIPOLYTICA;

AMYLASES;

AMMONIUM SULFATE; AMYLASE; STARCH; CULTURE MEDIUM; DNA BINDING PROTEIN; FUNGAL DNA; PDR1 PROTEIN, S CEREVISIAE; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CULTURE MEDIUM; DEGRADATION; ENZYME ACTIVITY; ENZYME MEMBRANE BIOREACTOR; ENZYME PURIFICATION; ENZYME RELEASE; FUNGAL STRAIN; MOLECULAR CLONING; NONHUMAN; PH; PRECIPITATION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SITOPHILUS ORYZAE; TEMPERATURE; YARROWIA LIPOLYTICA; ANIMAL; BIOSYNTHESIS; DNA FRAGMENTATION; DNA SEQUENCE; ENZYMOLOGY; GENETICS; INSECT; METABOLISM; ORYZA; SCANNING ELECTRON MICROSCOPY; YARROWIA;

ALPHA-AMYLASES; ANIMALS; CLONING, MOLECULAR; CULTURE MEDIA; DNA FRAGMENTATION; DNA, FUNGAL; DNA-BINDING PROTEINS; HYDROGEN-ION CONCENTRATION; INSECTS; MICROSCOPY, ELECTRON, SCANNING; ORYZA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS, DNA; STARCH; TRANSCRIPTION FACTORS; YARROWIA;

EID: 84925486584     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-6314-2     Document Type: Article

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