Purification and properties of a novel raw starch degrading-cyclodextrin glycosyltransferase from Klebsiella pneumoniae AS- 22

Enzyme and Microbial Technology

Volumn 28, Issue 9-10, 2001, Pages 735-743

  Gawande, B N ^a   Patkar, A Y ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

cyclodextrin; Cyclodextrin glycosyltransferase; Cyclodextrin production; Klebsiella pneumoniae; Properties; Purification; Raw starch

Indexed keywords

GEL PERMEATION CHROMATOGRAPHY; HYDROLYSIS; MOLECULAR WEIGHT; PURIFICATION; STARCH; ULTRAFILTRATION;

ISOELECTRIC POINT;

ENZYMES;

ALPHA CYCLODEXTRIN; CALCIUM CHLORIDE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; GLYCEROL; STARCH;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYCLIZATION; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBSTRATE; GEL FILTRATION CHROMATOGRAPHY; HYDROLYSIS; ISOELECTRIC POINT; KLEBSIELLA PNEUMONIAE; MOLECULAR WEIGHT; NONHUMAN; PH; THERMOSTABILITY; ULTRAFILTRATION;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE; NEGIBACTERIA; TRITICUM AESTIVUM;

EID: 0035821999     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(01)00347-7     Document Type: Article

References (42)

1. Production, characterization and application of cyclodextrins
2. Industrial applications of cyclodextrins
3. Cyclodextrin: Production, properties and applications
4. Production and industrial applications of beta cyclodextrin
5. Preparation and industrial production of cyclodextrins
6. Cyclodextrin glucanotransferase from Klebsiella pneumoniae. 1. Formation, purification and properties of the enzyme from Klebsiella pneumoniae M5 al
7. Purification and some properties of Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase
8. Application of factorial designs for optimization of cyclodextrin glycosyltransferase production from Klebsiella pneumoniae pneumoniae AS-22
9. Enzymatic production of α-cyclodextrin with the cyclomaltodextrin glucanotransferase of Klebsiella oxytoca 19-1
10. Cloning and expression of the Bacillus subtilis No. 313 γ-cyclodextrin forming CGTase gene in Escherichia coli
11. Biochemical and genetic characterization of a CGTase from an alkalophilic bacterium forming primarily γ-cyclodextrin
12. Purification and properties of cyclodextrin glycosyltransferase from Bacillus sp. AL-6
13. Purification and properties of cyclodextrin glucanotransferase from Brevibacterium sp no 9605
14. Enzymic synthesis of cyclodextrin in an heterogeneous enzyme reaction system containing insoluble extruded starch
15. Production of cyclodextrins from unliquefied corn starch using CGTase in a membrane bioreactor
16. Enzymatic production of cyclodextrins from milled corn starch in an ultrafiltration membrane bioreactor
17. Production of cyclodextrins using moderately heat-treated cornstarch
18. Production of cyclodextrins using raw corn starch without a pretreatment
19. The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251
20. Site-directed mutations in Tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity
21. Cyclodextrin formation by the thermostable α-amylase of Thermoanerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase
22. A spectrophotometric assay for the cyclization activity of cyclomaltohexaose (α-cyclodextrin) glucanotransferase
23. Determination of CGTase from Bacillus ohbensis and its optimum pH using HPLC
24. Purification and properties of cyclodextrin glycosyltransferase of an alkalophilic Bacillus sp.
25. Use of dinitro salicylic acid reagent for determination of reducing sugar
26. Protein measurement with folin phenol reagent
27. Starch gel for gel filtration
28. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
29. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
30. Rapid isoelectric focusing in a vertical polyacrylamide minigel system
31. Production and characterization of a thermostable bacterial cyclodextrin glycosyltransferase Bacillus sp.
32. Cyclomaltodextrin glucanotransferase from Bacillus circulans E 192 I. Purification and characterization of the enzyme
33. Purification and properties of cyclodextrin glucanotransferase from Brevibacterium sp. No. 9605
34. Comparative studies of CGTases from Bacillus ohbensis, Bacillus macerans and Bacillus circulans and production of cyclodextrins using those CGTases
35. Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species
36. Purification and properties of cyclomaltodextrin glucanotransferase from an alkalophilic Bacillus
37. Enzymology of cyclodextrins
38. The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251
39. Purification and properties of a novel raw starch degrading cyclomaltodextrin glucanotransferase from Bacillus firmus