Two-step method to isolate target recombinant protein from co-purified bacterial contaminant SlyD after immobilised metal affinity chromatography

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 853, Issue 1-2, 2007, Pages 314-319

  Parsy, Céline B ^a   Chapman, Caroline J ^b   Barnes, Antony C ^a   Robertson, John F ^b   Murray, Andrea ^a  

^a NOTTINGHAM CITY HOSPITAL   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Immobilized metal affinity chromatography; Recombinant protein; Size exclusion chromatography; SlyD

Indexed keywords

IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; RECOMBINANT EXPRESSION; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; BACTERIOLOGY; ENZYME IMMOBILIZATION; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY;

PROTEINS;

EPIDERMAL GROWTH FACTOR RECEPTOR 2; RECOMBINANT PROTEIN; SLYD PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CONTAMINATION; ELUTION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; SEPARATION TECHNIQUE;

BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI PROTEINS; METALS; MOLECULAR WEIGHT; PEPTIDYLPROLYL ISOMERASE; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 34250346111     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2007.03.046     Document Type: Article

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