Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications

Journal of Translational Medicine

Volumn 12, Issue 1, 2014, Pages

  Yan, Junrong ^a   Li, Guanghui ^a   Hu, Yonghong ^c   Ou, Weijun ^b   Wan, Yakun ^a,b  

^a SOUTHEAST UNIVERSITY   (China)

^b Jiangsu Nanobody Engineering and Research Center   (China)

^c NANJING TECH UNIVERSITY   (China)

Author keywords

Diagnostic application; Human prealbumin; Nanobody; Phage display; Trinucleotide cassettes

Indexed keywords

HORSERADISH PEROXIDASE; NANOBODY; NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN; OLIGONUCLEOTIDE; PREALBUMIN; TRINUCLEOTIDE; COMPLEMENTARITY DETERMINING REGION; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIGEN SPECIFICITY; ARTICLE; BIOTINYLATION; COMPLEMENTARITY DETERMINING REGION; COMPLEMENTARITY DETERMINING REGION 3; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE CONSTRUCT; PHAGE DISPLAY; PROTEIN ANALYSIS; AMINO ACID SEQUENCE; BACTERIOPHAGE; CHEMISTRY; GENETICS; IMMUNOLOGY; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; TRINUCLEOTIDE REPEAT;

AMINO ACID SEQUENCE; BACTERIOPHAGES; COMPLEMENTARITY DETERMINING REGIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; SINGLE-CHAIN ANTIBODIES; SINGLE-DOMAIN ANTIBODIES; TRINUCLEOTIDE REPEATS;

EID: 84923633871     PISSN: None     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/s12967-014-0343-6     Document Type: Article

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