Construction of a fully synthetic human scFv antibody library with CDR3 regions randomized by a split-mix-split method and its application

Journal of Biochemistry

Volumn 144, Issue 5, 2008, Pages 591-598

  Yin, Chang Cheng ^a,b   Ren, Li Li ^b   Zhu, Lin Lin ^b   Wang, Xiang Bin ^b   Zhang, Zhong ^b   Huang, Hua Liang ^b   Yan, Xi Yun ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b Beijing ABT Genetic Engineering Technology Co Ltd   (China)

Author keywords

Antibody library; Biopanning; Phage display; Randomization; Single chain antibody

Indexed keywords

AMINO ACID COMPOSITION; ANALYTIC METHOD; ANTIBODY LIBRARY; ARTICLE; COMPLEMENTARITY DETERMINING REGION; DNA SYNTHESIS; GENETIC SCREENING; MOLECULAR CLONING; NONHUMAN; RANDOMIZATION; SCFV ANTIBODY LIBRARY; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; SPLIT MIX SPLIT METHOD; STOP CODON;

AMINO ACID SEQUENCE; ANTIBODIES; COMPLEMENTARITY DETERMINING REGIONS; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, DNA;

EID: 55349108554     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvn103     Document Type: Article

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