Design and generation of synthetic antibody libraries for phage display

Methods in Molecular Biology

Volumn 1131, Issue , 2014, Pages 113-131

  Chen, Gang ^a   Sidhu, Sachdev S ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Diversity; Phage display; Protein engineering; Synthetic antibody library

Indexed keywords

MONOCLONAL ANTIBODY; OLIGONUCLEOTIDE;

ANTIBODY LIBRARY; ANTIBODY STRUCTURE; ARTICLE; BIOPANNING; COMPLEMENTARITY DETERMINING REGION; DRUG DESIGN; ELECTROPORATION; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROCESS OPTIMIZATION;

ANTIBODIES; COMPLEMENTARITY DETERMINING REGIONS; PEPTIDE LIBRARY;

EID: 84934439807     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-992-5_8     Document Type: Article

References (27)

1. Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495-497
2. Nelson AL, Dhimolea E, Reichert JM (2010) Development trends for human monoclonal antibody therapeutics. Nat Rev Drug Discov 9:767-774
3. Morrow KJ Jr (2012) The new generation of antibody therapeutics: current status and future prospects. Cambridge Healthtech Institute, Needham, MA
4. Bradbury ARM, Sidhu S, Dübel S et al (2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245-254
5. Sidhu SS, Fellouse FA (2006) Synthetic therapeutic antibodies. Nat Chem Biol 2:682-688
6. Smith GP (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228:1315-1317
7. Fellouse FA, Sidhu SS (2006) Making antibodies in bacteria. In: Howard GC, Kaser MR (eds) Making and using antibodies: a practical handbook. CRC, Boca Raton, FL, pp 157-180
8. Johnson G, Wu TT (2000) Kabat database and its applications: 30 years after the fi rst variability plot. Nucleic Acids Res 28:214-218
9. Fellouse FA, Li B, Compaan DM et al (2005) Molecular recognition by a binary code. J Mol Biol 348:1153-1162
10. Fellouse FA, Wiesmann C, Sidhu SS (2004) Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition. Proc Natl Acad Sci U S A 101: 12467-12472
11. Fellouse FA, Esaki K, Birtalan S et al (2007) High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries. J Mol Biol 373: 924-940
12. Birtalan S, Zhang Y, Fellouse FA et al (2008) The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies. J Mol Biol 377:1518-1528
13. Fisher RD, Ultsch M, Lingel A et al (2010) Structure of the complex between HER2 and an antibody paratope formed by side chains from tryptophan and serine. J Mol Biol 402: 217-229
14. Sidhu SS, Li B, Chen Y et al (2004) Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. J Mol Biol 338:299-310
15. Knappik A, Ge LM, Honegger A et al (2000) Fully synthetic human combinatorial antibody libraries (HUCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J Mol Biol 296:57-86
16. Persson H, Ye W, Wernimont A et al (2013) CDR-H3 diversity is not required for antigen recognition by synthetic antibodies. J Mol Biol 425:803-811
17. Karauzum H, Chen G, Abaandou L et al (2012) Synthetic human monoclonal antibodies toward staphylococcal enterotoxin B (SEB) protective against toxic shock syndrome. J Biol Chem 287:25203-25215
18. Koellhoffer JF, Chen G, Sandesara RG et al (2012) Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the Ebola virus envelope glycoprotein. Chembiochem 13:2549-2557
19. Colwill K, Gräslund S, Persson H et al (2011) A roadmap to generate renewable protein binders to the human proteome. Nat Methods 8:551-561
20. Laver JD, Ancevicius K, Sollazzo P et al (2012) Synthetic antibodies as tools to probe RNAbinding protein function. Mol Biosyst 8: 1650-1657
21. Qazi O, Rani M, Gnanam AJ et al (2011) Development of reagents and assays for the detection of pathogenic burkholderia species. Faraday Discuss 149:23-36
22. Kunkel TA, Roberts JD, Zakour RA (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367-382
23. Hopp TP, Prickett KS, Price VL et al (1988) A short polypeptide marker sequence useful for recombinant protein identifi cation and purifi -cation. Nat Biotechnol 6:1204-1210
24. Lee CV, Sidhu SS, Fuh G (2004) Bivalent antibody phage display mimics natural immunoglobulin. J Immunol Methods 284:119-132
25. Picken RN, Mazaitis AJ, Maas WK et al (1983) Nucleotide sequence of the gene for heatstable enterotoxin II of Escherichia coli . Infect Immun 42:269-275
26. Kabat EA, Wu TT, Perry HM et al (1991) Sequences of proteins of immunological interest, 5th edn. National Institutes of Health, Bethesda, MD
27. Lechner RL, Engler MJ, Richardson CC (1983) Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. J Biol Chem 258: 11174-11184