Probing substrate interactions in the active tunnel of a catalytically deficient cellobiohydrolase (Cel7)

Journal of Biological Chemistry

Volumn 290, Issue 4, 2015, Pages 2444-2454

  Colussi, Francieli ^a   Sorensen, Trine H ^a   Alasepp, Kadri ^a   Kari, Jeppe ^a   Cruys Bagger, Nicolaj ^a   Windahl, Michael S ^a,b   Olsen, Johan P ^a   Borch, Kim ^b   Westh, Peter ^a  

^a ROSKILDE UNIVERSITY   (Denmark)

^b NOVOZYMES A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSE; ENZYMES;

CELLO-OLIGOSACCHARIDES; CELLOBIOHYDROLASES; DEGREE OF POLYMERIZATION; ENZYME-SUBSTRATE INTERACTIONS; MOLECULAR DESCRIPTIONS; SUBSTRATE INTERACTION; THERMODYNAMIC FUNCTIONS; TITRATION CALORIMETRY;

LIGANDS;

CELLULOSE 1,4 BETA CELLOBIOSIDASE; CELLULOSE; FUNGAL PROTEIN; LIGAND; OLIGOSACCHARIDE; PROTEIN BINDING;

ADSORPTION KINETICS; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; DEGREE OF POLYMERIZATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; HYPOCREA JECORINA; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR DYNAMICS; NONHUMAN; POLYMERIZATION; RESIDUE ANALYSIS; TEMPERATURE SENSITIVITY; THERMODYNAMICS; ADSORPTION; CALORIMETRY; CATALYSIS; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; PROTEIN CONFORMATION; REGRESSION ANALYSIS; TEMPERATURE; TRICHODERMA;

HYPOCREA JECORINA;

ADSORPTION; CALORIMETRY; CATALYSIS; CATALYTIC DOMAIN; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; FUNGAL PROTEINS; HYDROLYSIS; LIGANDS; OLIGOSACCHARIDES; PROTEIN BINDING; PROTEIN CONFORMATION; REGRESSION ANALYSIS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMODYNAMICS; TRICHODERMA;

EID: 84921524879     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.624163     Document Type: Article

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