A rapid ceramide synthase activity using NBD-sphinganine and solid phase extraction

Journal of Lipid Research

Volumn 56, Issue 1, 2015, Pages 193-199

  Tidhar, Rotem ^a   Sims, Kacee ^b   Rosenfeld Gur, Eden ^a   Shaw, Walter ^b   Futerman, Anthony H ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b Avanti Polar Lipids Inc   (United States)

Author keywords

Ceramides; Chromatography; Enzymology; Fatty acyl CoA; In vitro assay; Long chain base; N acylation; Sphingolipids

Indexed keywords

CERAMIDE SYNTHASE; FLUORESCENT DYE; N [6 (7 NITROBENZO 2 OXA 1,3 DIAZOL 4 YL)]; SPHINGANINE; SYNTHETASE; UNCLASSIFIED DRUG; 4 CHLORO 7 NITROBENZOFURAZAN; CERAMIDE; DIHYDROCERAMIDE DESATURASE; N-(7-(4-NITROBENZO-2-OXA-1,3-DIAZOLE))-6-AMINOCAPROYL SPHINGOSINE; OXIDOREDUCTASE;

ARTICLE; CELL CYCLE REGULATION; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN CELL; HUMAN TISSUE; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; RELIABILITY; SEPARATION TECHNIQUE; SOLID PHASE EXTRACTION; THIN LAYER CHROMATOGRAPHY; ANALOGS AND DERIVATIVES; HEK293 CELL LINE; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; TIME FACTOR;

4-CHLORO-7-NITROBENZOFURAZAN; CERAMIDES; ENZYME ASSAYS; HEK293 CELLS; HUMANS; OXIDOREDUCTASES; SOLID PHASE EXTRACTION; TIME FACTORS;

EID: 84920507067     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D052001     Document Type: Article

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