A markerless gene replacement method for B. amyloliquefaciens LL3 and its use in genome reduction and improvement of poly-γ-glutamic acid production

Applied Microbiology and Biotechnology

Volumn 98, Issue 21, 2014, Pages 8963-8973

  Zhang, Wei ^a   Gao, Weixia ^a   Feng, Jun ^a   Zhang, Chi ^a   He, Yulian ^a   Cao, Mingfeng ^b   Li, Qiang ^a   Sun, Yang ^a   Yang, Chao ^a   Song, Cunjiang ^a   Wang, Shufang ^a  

^a NANKAI UNIVERSITY   (China)

^b Iowa State University   (United States)

Author keywords

Bacillus amyloliquefaciens; Gamma PGA; Genome reduction; Markerless gene replacement; RocR

Indexed keywords

AMINO ACIDS; BACTERIOLOGY; CELL PROLIFERATION; DNA SEQUENCES; GENE ENCODING; POLYMERASE CHAIN REACTION;

5-FLUOROURACIL (5-FU); BACILLUS AMYLOLIQUEFACIENS; GAMMA-PGA; GENE REPLACEMENT; POLY-GAMMA-GLUTAMIC ACID; ROCR; TRANSCRIPTIONAL REGULATOR; URACIL PHOSPHORIBOSYLTRANSFERASE;

DNA;

POLYGLUTAMIC ACID;

BACILLUS; GENE DELETION; GENE VECTOR; GENETIC SELECTION; GENETICS; METABOLIC ENGINEERING; METABOLISM; MICROBIAL GENETICS; MOLECULAR BIOLOGY; PLASMID; PROCEDURES;

BACILLUS; GENE DELETION; GENETIC VECTORS; GENETICS, MICROBIAL; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MOLECULAR BIOLOGY; PLASMIDS; POLYGLUTAMIC ACID; SELECTION, GENETIC;

EID: 84920254970     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5824-2     Document Type: Article

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