A simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable Bacillus amyloliquefaciens strains

Applied Microbiology and Biotechnology

Volumn 85, Issue 4, 2010, Pages 1201-1209

  Zakataeva, Natalia P ^a   Nikitina, Oksana V ^a   Gronskiy, Sergey V ^a   Romanenkov, Dmitriy V ^a   Livshits, Vitaliy A ^a  

^a Ajinomoto Genetika Research Institute   (Russian Federation)

Author keywords

Colony PCR analysis; Marker free genetic modifications; Naturally nontransformable Bacillus amyloliquefaciens; Point mutation; Replacement recombination; Thermosensitive rolling circle plasmid

Indexed keywords

BACILLUS AMYLOLIQUEFACIENS; GENETIC MODIFICATIONS; PCR ANALYSIS; POINT MUTATIONS; REPLACEMENT RECOMBINATION; THERMO SENSITIVE;

CHROMOSOMES; POLYMERASE CHAIN REACTION;

BACTERIOLOGY;

BACTERIAL DNA; NUCLEOTIDE; PLASMID VECTOR; PRIMER DNA;

BACTERIUM; CHROMOSOME; GENETIC ENGINEERING; GENETIC VARIATION; MUTATION; PHYSIOLOGY; PLASMID; POLYMERASE CHAIN REACTION;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; DNA MODIFICATION; DNA RECOMBINATION; DNA SCREENING; ELECTROTRANSFORMATION; GENE DELETION; GENE INSERTION; GENE MUTATION; GENETIC RECOMBINATION; GENETIC TRANSDUCTION; HEAT SENSITIVITY; METABOLIC ENGINEERING; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION;

BACILLUS; BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; GENETIC MARKERS; GENETIC VECTORS; GLUCOSE 1-DEHYDROGENASE; MEMBRANE PROTEINS; MUTAGENESIS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROMOTER REGIONS, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; TRANSFORMATION, BACTERIAL;

BACILLUS AMYLOLIQUEFACIENS;

EID: 76649107894     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2276-1     Document Type: Article

References (31)

1. C Anagnostopoulos J Spizizen 1961 Requirements for transformation in Bacillus subtilis J Bacteriol 81 741 746 1:CAS:528:DyaF3MXpsVSnsg%3D%3D
2. M Arnaud A Chastanet M Débarbouillé 2004 New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria Appl Environ Microbiol 70 6887 6891 10.1128/AEM.70.11. 6887-6891.2004 1:CAS:528:DC%2BD2cXhtVSju7rF (Pubitemid 39518603)
3. T Bhowmik L Fernández JL Steele 1993 Gene replacement in Lactobacillus helveticus J Bacteriol 175 6341 6344 1:CAS:528:DyaK2cXhs1Sm
4. I Biswas A Gruss SD Ehrlich E Maguin 1993 High-efficiency gene inactivation and replacement system for gram-positive bacteria J Bacteriol 175 3628 3635 1:CAS:528:DyaK3sXkvVOntbc%3D
5. AE Bloor RM Cranenburgh 2006 An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes Appl Environ Microbiol 72 2520 2525 10.1128/AEM.72.4.2520-2525.2006 1:CAS:528:DC%2BD28XktFagtbk%3D
6. A Brans P Filée A Chevigné A Claessens B Joris 2004 New integrative method to generate Bacillus subtilis recombinant strains free of selection markers Appl Environ Microbiol 70 7241 7250 10.1128/AEM.70.12.7241- 7250.2004 1:CAS:528:DC%2BD2cXhtFaitrzF (Pubitemid 39643789)
7. XH Chen A Koumoutsi R Scholz A Eisenreich K Schneider I Heinemeyer B Morgenstern B Voss WR Hess O Reva H Junge B Voigt PR Jungblut J Vater R Süssmuth H Liesegang A Strittmatter G Gottschalk R Borriss 2007 Comparative analysis of the complete genome sequence of the plant growth promoting Bacillus amyloliquefaciens FZB42 Nat Biotechnol 25 1007 1014 10.1038/nbt1325 1:CAS:528:DC%2BD2sXhtVWksLvL (Pubitemid 47517636)
8. H Coukoulis LL Campbell 1971 Transformation in Bacillus amyloliquefaciens J Bacteriol 105 319 322 1:CAS:528:DyaE3MXltVymsg%3D%3D
9. C Fabret SD Ehrlich P Noirot 2002 A new mutation delivery system for genome-scale approaches in Bacillus subtilis Mol Microbiol 46 25 36 10.1046/j.1365-2958.2002.03140.x 1:CAS:528:DC%2BD38XotFSiu7c%3D (Pubitemid 35231944)
10. MM Huang N Arnheim MF Goodman 1992 Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR Nucleic Acids Res 20 4567 4573 10.1093/nar/20.17.4567 1:CAS:528:DyaK3sXltlWqtQ%3D%3D
11. BK Janes S Stibitz 2006 Routine markerless gene replacement in Bacillus anthracis Infect Immun 74 1949 1953 10.1128/IAI.74.3.1949-1953.2006 1:CAS:528:DC%2BD28XmtFWjs7w%3D (Pubitemid 43346834)
12. Jomantas JAV, Fiodorova JA, Abalakina EG, Kozlov YI (1991) Genetics of Bacillus amyloliquefaciens. 6th International Conference on Bacilli, Stanford, Calif., abstr. no T7
13. S Liu K Endo K Ara K Ozaki N Ogasawara 2008 Introduction of marker-free deletions in Bacillus subtilis using the AraR repressor and the ara promoter Microbiology 154 2562 2570 10.1099/mic.0.2008/016881-0 1:CAS:528: DC%2BD1cXhtF2gtbzI
14. E Maguin H Prevost SD Ehrlich A Gruss 1996 Efficient insertional mutagenesis in lactococci and other Gram-positive bacteria J Bacteriol 178 931 935 1:CAS:528:DyaK28XnsFWhug%3D%3D
15. M Mandal RR Breaker 2004 Adenine riboswitches and gene activation by disruption of a transcription terminator Nat Struct Mol Biol 11 29 35 10.1038/nsmb710 1:CAS:528:DC%2BD2cXhtVKqtbc%3D (Pubitemid 38173438)
16. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
17. P Noirot MA Petit SD Ehrlich 1987 Plasmid replication stimulates DNA recombination in Bacillus subtilis J Mol Biol 196 39 48 10.1016/0022-2836(87) 90509-2 1:CAS:528:DyaL2sXkvFWjtL8%3D
18. P Nygaard HH Saxild 2005 The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size J Bacteriol 187 791 794 10.1128/JB.187.2.791-794.2005 1:CAS:528:DC%2BD2MXmtVGjsA%3D%3D (Pubitemid 40096268)
19. RF Ramaley N Vasantha 1983 Glycerol protection and purification of Bacillus subtilis glucose dehydrogenase J Biol Chem 258 12558 12565 1:CAS:528:DyaL3sXlvFSgu7g%3D
20. PN Razer CP Moran Jr 1988 Compartment-specific transcription in Bacillus subtilis: identification of the promoter for gdh J Bacteriol 170 5086 5092
21. Sambrook J, Russell DW (2001) Molecular cloning: laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
22. KY Shatalin AA Neyfakh 2005 Efficient gene inactivation in Bacillus anthracis FEMS Microbiol Lett 15 315 319 10.1016/j.femsle.2005.03.029 (Pubitemid 40523649)
23. SS Sommer JD Cassady JL Sobell CD Bottema 1989 A novel method for detecting point mutations or polymorphisms and its application to population screening for carriers of phenylketonuria Mayo Clin Proc 64 1361 1372 1:STN:280:DyaK3c%2FotV2ksQ%3D%3D
24. J Vehmaanperä 1988 Transformation of Bacillus amyloliquefaciens protoplasts with plasmid DNA FEMS Microbiol Lett 49 101 105 10.1111/j.1574-6968. 1988.tb02689.x
25. J Vehmaanperä 1989 Transformation of Bacillus amyloliquefaciens by electroporation FEMS Microbiol Lett 52 165 169 10.1111/j.1574-6968.1989.tb03572. x
26. J Vehmaanperä G Steinborn J Hofemeister 1991 Genetic manipulation of Bacillus amyloliquefaciens J Biotechnol 19 221 240 10.1016/0168-1656(91)90061-Y
27. B Waschkau J Waldeck S Wieland R Eichstädt F Meinhardt 2008 Generation of readily transformable Bacillus licheniformis mutants Appl Microbiol Biotechnol 78 181 188 10.1007/s00253-007-1278-0 1:CAS:528: DC%2BD1cXot1Klsw%3D%3D
28. DY Wu L Ugozzoli BK Pal RB Wallace 1989 Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anemia Proc Natl Acad Sci U S A 86 2757 2760 10.1073/pnas.86.8.2757 1:CAS:528:DyaL1MXktlKjs7k%3D (Pubitemid 19112620)
29. X Yan HJ Yu Q Hong SP Li 2008 Cre/lox system and PCR-based genome engineering in Bacillus subtilis Appl Environ Microbiol 74 5556 5562 10.1128/AEM.01156-08 1:CAS:528:DC%2BD1cXhtV2ru7nP
30. NP Zakataeva SV Gronskiy AS Sheremet EA Kutukova AE Novikova VA Livshits 2007 A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides Res Microbiol 158 659 665 10.1016/j.resmic.2007.08.003 1:CAS:528:DC%2BD2sXhsVWjtLrO (Pubitemid 350213376)
31. XZ Zhang X Yan ZL Cui Q Hong SP Li 2006 mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis Nucleic Acids Res 34 e71 10.1093/nar/gkl358 (Pubitemid 43985652)