Enhanced clavulanic acid production in Streptomyces clavuligerus NRRL3585 by overexpression of regulatory genes

Biotechnology and Bioprocess Engineering

Volumn 11, Issue 2, 2006, Pages 116-120

  Hung, Trinh Viet ^a   Ishida, Kenji ^a   Parajuli, Niranjan ^a   Liou, Kwangkyoung ^a   Lee, Hei Chan ^a   Sohng, Jae Kyung ^a  

^a SunMoon University   (South Korea)

Author keywords

afsR p; ccaR; claR; Clavulanic acid; Production; Regulatory gene; Streptomyces clavuligerus

Indexed keywords

CEPHAMYCIN C; CLAVULANIC ACID; PROTEIN AFSR P; PROTEIN CCAR; PROTEIN CLAR; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DRUG STRUCTURE; DRUG SYNTHESIS; FUNGAL GENETICS; GENE OVEREXPRESSION; NONHUMAN; PROMOTER REGION; RECOMBINANT PLASMID; REGULATOR GENE; STREPTOMYCES CLAVULIGERUS; WILD TYPE;

STREPTOMYCES CLAVULIGERUS; STREPTOMYCES PEUCETIUS;

EID: 33646408415     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931894     Document Type: Article

References (28)

1. Romero, J., P. Liras, and J. F. Martin (1984) Dissociation of cephamycin and clavulanic acid biosynthesis in Streptomyces clavuligerus. Appl. Microbiol. Biotechnol. 20: 318-325.
2. Christopher, W. (2003) Antibiotics. pp. 107-123. ASM Press, Washington, D.C., USA.
3. Foulstone, M. and C. Reading (1982) Assay of amoxicillin and clavulanic acid, the components of augmentin, in biological fluids with high-performance liquid chromatography. Antimicrob. Agents Chemother. 22: 753-762.
4. Paradkar, A. S., K. A. Aidoo, and S. E. Jensen (1998) A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Mol. Microbiol. 27: 831-843.
5. Perez-Redondo, R., A. Rodriguez-Garcia, J. F. Martin, and P. Liras (1998) The claR gene of Streptomyces clavuligerus, encoding a LysR-type regulatory protein controlling clavulanic acid biosynthesis, is linked to the clavulanate-9-aldehyde reductase (car) gene. Gene 211: 311-321.
6. Alexander, D. C. and S. E. Jensen (1998) Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein. J. Bacteriol. 180: 4068-4079.
7. Ward, J. M. and J. E. Hodgson (1993) The biosynthetic genes for clavulanic acid and cephamycin production occur as a 'super-cluster' in three Streptomyces. FEMS Microbiol. Lett. 110: 239-242.
8. Perez-Liarena, F. J., P. Liras, A. Rodriguez-Garcia, J. F. Martin (1997) A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both β-lactam compounds. J. Bacteriol. 179: 2053-2059.
9. Jensen, S. E., A. S. Paradkar, R. H. Mosher, C. Anders, P. H. Beatty, M. J. Brumlik, A. Griffin, and B. Barton (2004) Five additional genes are involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. Antimicrob. Agents Chemother. 48: 192-202.
10. Madduri, K. and C. R. Hutchinson (1995) Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. J. Bacteriol. 177: 1208-1215.
11. Iwata-Reuyl, D. and C. A. Townsend (1992) Common origin of clavulanic acid and other clavam metabolites in Streptomyces. J. Am. Chem. Soc. 114: 2762-2763.
12. Townsend, C. A. and M. F. Ho (1985) Biosynthesis of clavulanic acid: origin of the C3 unit. J. Am. Chem. Soc. 107: 1066-1068.
13. Salowe, S. P., E. N. Marsh, and C. A. Townsend (1990) Purification and characterization of clavaminate synthase from Streptomyces clavuligerus: an unusual oxidative enzyme in natural product biosynthesis. Biochemistry 29: 6499-6508.
14. Townsend, C. A. (2002) New reactions in clavulanic acid biosynthesis. Curr. Opin. Chem. Biol. 6: 583-589.
15. Marsh, E. N., M. D. T. Chang, and C. A. Townsend (1992) Two isozymes of clavaminate synthase central to clavulanic acid formation: cloning and sequencing of both genes from Streptomyces clavuligerus. Biochemistry 31: 12648-12657.
16. Mosher, R. H., A. S. Paradkar, C. Anders, B. Barton, and S. E. Jensen (1999) Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus. Antimicrob. Agents Chemother. 43: 1215-1224.
17. Paradkar, A. S. and S. E. Jensen (1995) Functional analysis of the gene encoding the clavaminate synthase 2 isoenzyme involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. J. Bacteriol. 177: 1307-1314.
18. Matsumoto, A., H. Ishizuka, T. Beppu, and S. Horinouchi (1995) Involvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in Streptomyces coelicolor A3(2). Actinomycetologica 9: 37-43.
19. Kim, C. Y., H. J. Park, and E. S. Kim (2005) Proteomics-driven identification of putative AfsR2-target proteins stimulating antibiotic biosynthesis in Streptomyces lividans. Biotechnol. Bioprocess Eng. 10: 248-253.
20. Umeyama, T., P. C. Lee, K. Ueda, and S. Horinouchi (1999) An AfsK/AfsR system involved in the response of aerial mycelium formation to glucose in Streptomyces griseus. Microbiology 145: 2281-2292.
21. Niranjan, P., T. V. Hung, K. Ishida, T. T. Hang, H. C. Lee, K. K. Liou, and J. K. Sohng (2004) Identification and characterization of the afsR homologue regulatory gene from Streptomyces peucetius ATCC 27952. Res. Microbiol. 156: 707-712.
22. Hung, T. V., K. Ishida, H. C. Lee, and J. K. Sohng (2004) Expression of ccaR, claR and cas2 in Streptomyces clavuligerus shows overproduction of clavulanic acid. J. Biomoel. Reconstr. 1: 37-44.
23. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood (2000) Practical Streptomyces Genetics. pp. 125-200. The John Innes Foundation, Norwich, UK.
24. Kim, J. H., J. S. Lim, and S. W. Kim (2004) The improvement of cephalosporin C production by fed-batch culture of Cephalosporium acremonium M25 using rice oil. Biotechnol. Bioprocess Eng. 9: 459-464.
25. Lee, M. S., J. S. Lim, C. H. Kim, K. K. Oh, S. I. Hung, and S. W. Kim (2001) Effecs of nutrients and culture conditions on morhology in the seed culture of Cephalosporium acremonium ATCC 20339. Biotechnol. Bioprocess Eng. 6: 156-160.
26. Basundhara, S., T. J. Oh, L. Rajan, K. K. Liou, H. C. Lee, C. G. Kim, and S. K. Sohng (2004) Neocarzinostatin naphthoate synthase: an unique iterative type I PKS from neocarzinostatin producer Streptomyces carzinostaticus. FEBS Lett. 566: 201-206.
27. Garcia-Dominguez, M., J. F. Martin, B. Mahro, A. L. Demain, and P. Liras (1987) Efficient plasmid transformation of the β-lactam producer Streptomyces clavuligerus. Appl. Environ. Microbiol. 53: 1376-1381.
28. Arias, P., M. A. Fernandez-Moreno, and F. Malpartida (1999) Characterization of the pathway-specific positive transcriptional regulator for actinorhodin biosynthesis in Streptomyces coelicolor A3(2) as a DNA-binding protein. J. Bacteriol. 181: 6958-6968.