Structural and spectroscopic characterization of P450 BM3 mutants with unprecedented P450 heme iron ligand sets: New heme ligation states influence conformational equilibria in P450 BM3

Journal of Biological Chemistry

Volumn 282, Issue 1, 2007, Pages 564-572

  Girvan, Hazel M ^a   Seward, Harriet E ^c   Toogood, Helen S ^b   Cheesman, Myles R ^d   Leys, David ^c   Munro, Andrew W ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

^d UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ATOMIC STRUCTURES; CONFORMATIONAL HETEROGENEITY; MULTIDOMAIN FLAVOCYTOCHROME;

CONFORMATIONS; IRON; MUTAGENESIS; PHASE EQUILIBRIA; PIGMENTS; SPECTROSCOPIC ANALYSIS;

LIGANDS;

CYTOCHROME P450 BM3; HEME; IRON; BACTERIAL PROTEIN; BIFUNCTIONAL REDUCTASE CYP102, BACILLUS MEGATERIUM; CYTOCHROME P450; LIGAND; MIXED FUNCTION OXIDASE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; ELECTRON SPIN RESONANCE; INFRARED SPECTROSCOPY; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN STRUCTURE; SPECTROSCOPY; VALIDATION PROCESS; WILD TYPE; CATALYSIS; CHEMISTRY; CONFORMATION; CRYSTALLIZATION; GENETICS; MUTAGENESIS; MUTATION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

BACTERIAL PROTEINS; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON SPIN RESONANCE SPECTROSCOPY; HEME; IRON; LIGANDS; MIXED FUNCTION OXYGENASES; MOLECULAR CONFORMATION; MUTAGENESIS; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SPECTROPHOTOMETRY;

EID: 33846958772     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M607949200     Document Type: Article

References (42)

1. Denisov, I. G., Makris, T. M., Sligar, S. G., and Schlichtling, I. (2005) Chem. Rev. 105, 2253-2277
2. Munro, A. W., and Lindsay, J. G. (1996) Mol. Microbiol. 20, 1115-1125
3. Guengerich, F. P. (2001) Chem. Res. Toxicol. 14, 611-650
4. McLean, K. J., Sabri, M., Marshall, K. R., Lawson, R. J., Lewis, D. G., Clift, D., Balding, P. R., Dunford, A. J., Warman, A. J., McVey, J. P., Quinn, A. M., Sutcliffe, M. J., Scrutton, N. S., and Munro, A. W. (2005) Biochem. Soc. Trans. 33, 796-801
5. Narhi, L. O., and Fulco, A. J. (1987) J. Biol. Chem. 262, 6683-6690
6. Miles, J. S., Munro, A. W., Rospendowski, B. N., Smith, W. E., McKnight, J. E., and Thomson, A. J. (1992) Biochem. J. 288, 503-509
7. Oster, T., Boddupalli, S. S., and Peterson, J. A. (1991) J. Biol. Chem. 266, 22718-22725
8. Noble, M. A., Miles, C. S., Chapman, S. K., Lysek, D. A., MacKay, A. C., Reid, G. A., Hanzlik, R. P., and Munro, A. W. (1999) Biochem. J. 339, 371-379
9. Daff, S. N., Chapman, S. K., Turner, K. L., Holt, R. A., Govindaraj, S., Poulos, T. L., and Munro, A. W. (1997) Biochemistry 36, 13816-13823
10. Neeli, R., Girvan, H. M., Lawrence, A., Warren, M. J., Leys, D., Scrutton, N. S., and Munro, A. W. (2005) FEBS Lett. 579, 5582-5588
11. Siddhanta, U., Presta, A., Fan, B., Wolan, D., Rousseau, D. L., and Stuehr, D. J. (1998) J. Biol. Chem. 273, 18950-18958
12. Stuehr, D. J., Santolini, J., Wang, Z. Q., Wei, C. C., and Adak, S. (2004) J. Biol. Chem. 279, 36167-36170
13. Sundaramoorthy, M., Terner, J., and Poulos, T. L. (1995) Structure 3, 1367-1377
14. Cheesman, M. R., Little, P. J., and Berks, B. C. (2001) Biochemistry 40, 10562-10569
15. Meiyer, M., Janosik, M., Kery, V., Kraus, J. P., and Burkhard, P. (2001) EMBO J. 20, 3910-3916
16. Roberts, G. P., Kerby, R. L., Youn, H., and Conrad, M. (2005) J. Inorg. Biochem. 99, 280-292
17. Lawson, R. J., Leys, D., Sutcliffe, M. J., Kemp, C. A., Cheesman, M. R., Smith, S. J., Clarkson, J., Smith, W. E., Haq, I., Perkins, J. B., and Munro, A. W. (2004) 43, 12410-12426
18. Dawson, J. H., Andersson, L. A., and Sono, M. (1982) J. Biol. Chem. 257, 3606-3617
19. Sono, M., Hager, L. P., and Dawson, J. H. (1991) Biochim. Biophys. Acta 1078, 351-359
20. Cheesman, M. R., Zumft, W. G., and Thomson, A. J. (1998) Biochemistry 37, 3994-4000
21. Girvan, H. M., Marshall, K. R., Lawson, R. J., Leys, D., Joyce, M. G., Clarkson, J., Smith, W. E., Cheesman, M. R., and Munro, A. W. (2004) J. Biol. Chem. 279, 23274-23286
22. Joyce, M. G., Girvan, H. M., Munro, A. W., and Leys, D. (2004) J. Biol. Chem. 279, 23287-23293
23. Neeli, R., Roitel, O., Scrutton, N. S., and Munro, A. W. (2005) J. Biol. Chem. 280, 17634-17644
24. Berry, E. A., and Trumpower, B. L. (1987) Anal Biochem. 161, 1-15
25. Otwinowski, Z., and Minor, W. (1997) Methods Enzymol. 276, 307-318
26. Navaza, J. (2001) Acta Crystallogr. Sect. D Biol. Crystallogr. 57, 1367-1372
27. Murshudov, G. N., Vagin, A. A., and Dodson, E. J. (1997) Acta Crystallogr. Sect. D Biol. Crystallogr. 53, 240-255
28. Wuttke, D. S. (1994) Preparation, Characterization and Intramolecular Electron-transfer Studies of Ruthenium-modified Cytochromes c. Ph.D. thesis, California Institute of Technology, Pasadena, CA
29. Dhawan, I. K., Shelver, D., Thorsteinsson, M. V., Roberts, G. P., and Johnson, M. K. (1999) Biochemistry 38, 12805-12813
30. Limburg, J., LeBrun, L. A., and Ortiz de Montellano, P. R. (2005) Biochemistry 44, 4091-4099
31. Gadsby, P. M. A., and Thomson, A. J. (1990) J. Am. Chem. Soc. 112, 5003-5011
32. McKnight, J., Cheesman, M. R., Thomson, A. J., Miles, J. S., and Munro, A. W. (1993) Eur. J. Biochem. 213, 683-687
33. Ravichandran, K. G., Boddupalli, S. S., Hasemann, C. A., Peterson, J. A., and Diesenhofer, J. (1993) Science 261, 731-736
34. Haines, D. C., Tomchick, D. R., Machius, M., and Peterson, J. A. (2001) Biochemistry 40, 13456-13465
35. Sevrioukova, I. F., Li, H., Zhang, H., Peterson, J. A., and Poulos, T. L. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 1863-1868
36. Leys, D., Mowat, C. G., McLean, K. J., Richmond, A., Chapman, S. K., Walkinshaw, M. D., and Munro, A. W. (2003) J. Biol. Chem. 278, 5141-5147
37. McLean, K. J., Cheesman, M. R., Rivers, S. L., Richmond, A., Leys, D., Chapman, S. K., Reid, G. A., Price, N. C., Kelly, S. M., Clarkson, J., Smith, W. E., and Munro, A. W. (2002) J. Inorg. Biochem. 91, 527-541
38. Omura, T., and Sato, R. (1964) J. Biol. Chem. 239, 2370-2378
39. Quaroni, L. G., Seward, H. E., McLean, K. J., Girvan, H. M., Ost, T. W., Noble, M. A., Kelly, S. M., Price, N. C., Cheesman, M. R., Smith, W. E., and Munro, A. W. (2004) Biochemistry 43, 16416-16431
40. Youn, H., Conrad, M., Chung, S.-Y., and Roberts, G. P. (2006) Biochem. Biophys. Res. Commun. 348, 345-350
41. Li, H., and Poulos, T. L. (1997) Nat. Struct. Biol. 4, 140-146
42. Baer, B. R., Schuman, J. T., Campbell, A. P., Cheesman, M. J., Nakano, M., Moguilevsky, N., Kunze, K. L., and Rettie, A. E. (2005) Biochemistry 44, 13914-13920