Characterization and study of transgenic cultivars by capillary and microchip electrophoresis

International Journal of Molecular Sciences

Volumn 15, Issue 12, 2014, Pages 23851-23877

  Vega, Elena Domínguez ^a   Marina, Maria Luisa ^b  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Capillary electrophoresis; Food; GMO; Microchip electrophoresis; Safety assessment; Transgenic cultivars

Indexed keywords

CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DNA DETERMINATION; DNA MODIFICATION; GENE AMPLIFICATION; GENE INTERACTION; HYDROLYSIS; LIMIT OF DETECTION; MAIZE; MICROCHIP ELECTROPHORESIS; MULTIPLEX LIGATION DEPENDENT PROBE AMPLIFICATION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PROTEIN ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; RICE; SOYBEAN; TIME OF FLIGHT MASS SPECTROMETRY; TRANSGENIC PLANT; YEAST; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; METABOLOMICS; PROCEDURES; PROTEOMICS;

ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, MICROCHIP; GENE EXPRESSION PROFILING; METABOLOMICS; PLANTS, GENETICALLY MODIFIED; PROTEOMICS;

EID: 84919819888     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms151223851     Document Type: Review

References (57)

1. Holst-Jensen, A. Testing for genetically modified organisms (GMOs): Past, present and future perspectives. Biotechnol. Adv. 2009, 27, 1071-1082.
2. SmartStax™. Available online: http://msdssearch.dow.com/PublishedLiteratureDOWCOM/dh_03e3?0901b803803e393d.pdf?filepath=productsafety/pdfs/noreg/233-00616.pdf&fromPage=GetDoc (accessed on 15 October 2014).
3. Kuiper, H.A.; Kleter, G.A.; Noteborn, H.; Kok, E.J. Assessment of the food safety issues related to genetically modified foods. Plant J. 2001, 27, 503-528.
4. Kuiper, H.A.; Kok, E.J.; Engel, K.H. Exploitation of molecular profiling techniques for GM food safety assessment. Curr. Opin. Biotechnol. 2003, 14, 238-243.
5. Valdes, A.; Simo, C.; Ibanez, C.; Garcia-Canas, V. Foodomics strategies for the analysis of transgenic foods. Trac-Trends Anal. Chem. 2013, 52, 2-15.
6. Ishikawa, T.; Takahara, K.; Hirabayashi, T.; Matsumura, H.; Fujisawa, S.; Terauchi, R.; Uchimiya, H.; Kawai-Yamada, M. Metabolome analysis of response to oxidative stress in rice suspension cells overexpressing cell death suppressor bax inhibitor-1. Plant Cell Physiol. 2010, 51, 9-20.
7. Takahara, K.; Kasajima, I.; Takahashi, H.; Hashida, S.-N.; Itami, T.; Onodera, H.; Toki, S.; Yanagisawa, S.; Kawai-Yamada, M.; Uchimiya, H. Metabolome and photochemical analysis of rice plants overexpressing arabidopsis nad kinase gene. Plant Physiol. 2010, 152, 1863-1873.
8. Regulation (EC) 1829/2003. Available online: http://ec.europa.eu/food/food/animalnutrition?labelling/Reg_1829_2003_en.pdf (accessed on 15 October 2014).
9. Regulation (EC) 1830/2003. Available online: http://eur-lex.europa.eu/LexUriServ/LexUriServ. do?uri=OJ:L:2003:268:0024:0028:EN:PDF (accessed on 15 October 2014).
10. Michelini, E.; Simoni, P.; Cevenini, L.; Mezzanotte, L.; Roda, A. New trends in bioanalytical tools for the detection of genetically modified organisms: An update. Anal. Bioanal. Chem. 2008, 392, 355-367.
11. Marmiroli, N.; Maestri, E.; Gulli, M.; Malcevschi, A.; Peano, C.; Bordoni, R.; de Bellis, G. Methods for detection of GMOs in food and feed. Anal. Bioanal. Chem. 2008, 392, 369-384.
12. Elenis, D.S.; Kalogianni, D.P.; Glynou, K.; Ioannou, P.C.; Christopoulos, T.K. Advances in molecular techniques for the detection and quantification of genetically modified organisms. Anal. Bioanal. Chem. 2008, 392, 347-354.
13. Morisset, D.; Stebih, D.; Cankar, K.; Zel, J.; Gruden, K. Alternative DNA amplification methods to PCR and their application in GMO detection: A review. Eur. Food Res. Technol. 2008, 227, 1287-1297.
14. Chaouachi, M.; Chupeau, G.; Berapd, A.; McKhann, H.; Romaniuk, M.; Giancola, S.; Laval, V.; Bertheau, Y.; Brunel, D. A high-throughput multiplex method adapted for gmo detection. J. Agric. Food Chem. 2008, 56, 11596-11606.
15. Ahmed, F.E. Detection of genetically modified organisms in foods. Trends Biotechnol. 2002, 20, 215-223.
16. Garcia-Cañas, V.; Simó, C.; Leon, C.; Ibañez, E.; Cifuentes, A. MS-based analytical methodologies to characterize genetically modified crops. Mass Spectrom. Rev. 2011, 30, 396-416.
17. Kok, M.G.M.; Ruijken, M.M.A.; Swann, J.R.; Wilson, I.D.; Somsen, G.W.; de Jong, G.J. Anionic metabolic profiling of urine from antibiotic-treated rats by capillary electrophoresis-mass spectrometry. Anal. Bioanal. Chem. 2013, 405, 2585-2594.
18. Trojanowicz, M.; Latoszek, A.; Pobozy, E. Analysis of genetically modified food using high-performance separation methods. Anal. Lett. 2010, 43, 1653-1679.
19. Guo, L.; Qiu, B.; Xiao, X.; Chen, G. Applications of capillary electrophoresis and microchip capillary electrophoresis for detection of genetically modified organisms. Food Sci. Biotechnol. 2009, 18, 823-832.
20. García-Cañas, V.; Cifuentes, A.; González, R. Detection of genetically modified organisms in foods by DNA amplification techniques. Crit. Rev. Food Sci. Nutr. 2004, 44, 425-436.
21. Holck, A.; Pedersen, B.O.; Heir, E. Detection of five novel gmo maize events by qualitative, multiplex PCR and fluorescence capillary gel electrophoresis. Eur. Food Res. Technol. 2010, 231, 475-483.
22. Holck, A.L.; Pedersen, B.O. Simple, sensitive, accurate multiplex quantitative competitive PCR with capillary electrophoresis detection for the determination of genetically modified maize. Eur. Food Res. Technol. 2011, 233, 951-961.
23. Holck, A.L.; Dromtorp, S.M.; Heir, E. Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events. Eur. Food Res. Technol. 2009, 230, 185-194.
24. Schouten, J.P.; McElgunn, C.J.; Waaijer, R.; Zwijnenburg, D.; Diepvens, F.; Pals, G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res. 2002, 30, e57.
25. Garcia-Cañas, V.; Mondello, M.; Cifuentes, A. Simultaneous detection of genetically modified organisms by multiplex ligation-dependent genome amplification and capillary gel electrophoresis with laser-induced fluorescence. Electrophoresis 2010, 31, 2249-2259.
26. Garcia-Cañas, V.; Mondello, M.; Cifuentes, A. Combining ligation reaction and capillary gel electrophoresis to obtain reliable long DNA probes. J. Sep. Sci. 2011, 34, 1011-1019.
27. Hecker, K.H.; Rill, R.L. Error analysis of chemically synthesized polynucleotides. BioTechniques 1998, 24, 256-260.
28. Basak, S.; Ehtesham, N.Z.; Sesikeran, B.; Ghosh, S. Detection and identification of transgenic elements by fluorescent-PCR-based capillary gel electrophoresis in genetically modified cotton and soybean. J. AOAC Int. 2014, 97, 159-165.
29. Nadal, A.; Esteve, T.; Pla, M. Multiplex polymerase chain reaction-capillary gel electrophoresis: A promising tool for gmo screening-assay for simultaneous detection of five genetically modified cotton events and species. J. AOAC Int. 2009, 92, 765-772.
30. Leon, C.; Garcia-Cañas, V.; González, R.; Morales, P.; Cifuentes, A. Fast and sensitive detection of genetically modified yeasts in wine. J. Chromatogr. 2011, 1218, 7550-7556.
31. González-Ramos, D.; Cebollero, E.; González, R. A recombinant saccharomyces cerevisiae strain over-producing mannoproteins stabilizes wine against protein haze. Appl. Environ. Microbiol. 2008, 74, 5533-5540.
32. Jiang, C.; Xu, S.; Zhang, S.; Jia, L. Chitosan functionalized magnetic particle-assisted detection of genetically modified soybeans based on polymerase chain reaction and capillary electrophoresis. Anal. Biochem. 2012, 420, 20-25.
33. Guo, L.; Yang, H.; Qiu, B.; Xiao, X.; Xue, L.; Kim, D.; Chen, G. Capillary electrophoresis with electrochemiluminescent detection for highly sensitive assay of genetically modified organisms. Anal. Chem. 2009, 81, 9578-9584.
34. Stojkovic, M.; Uda, N.R.; Brodmann, P.; Popovic, M.; Hauser, P.C. Determination of pcr products by CE with contactless conductivity detection. J. Sep. Sci. 2012, 35, 3509-3513.
35. Raymond, P.; Gendron, L.; Khalf, M.; Paul, S.; Dibley, K.L.; Bhat, S.; Xie, V.R.D.; Partis, L.; Moreau, M.-E.; Dollard, C., et al. Detection and identification of multiple genetically modified events using DNA insert fingerprinting. Anal. Bioanal. Chem. 2010, 396, 2091-2102.
36. Guo, J.; Yang, L.; Chen, L.; Morisset, D.; Li, X.; Pan, L.; Zhang, D. Mpic: A high-throughput analytical method for multiple DNA targets. Anal. Chem. 2011, 83, 1579-1586.
37. Haselberg, R.; de Jong, G.J.; Somsen, G.W. Capillary electrophoresis-mass spectrometry for the analysis of intact proteins. J. Chromatogr. 2007, 1159, 81-109.
38. Pobozy, E.; Sentkowska, A.; Piskor, A. Comparison of three modifications of fused-silica capillaries and untreated capillaries for protein profiling of maize extracts by capillary electrophoresis. J. Sep. Sci. 2014, 37, 2388-2394.
39. Latoszek, A.; Garcia-Ruiz, C.; Marina, M.L.; de la Mata, F.J.; Gómez, R.; Rasines, B.; Cifuentes, A.; Pobozy, E.; Trojanowicz, M. Modification of resolution in capillary electrophoresis for protein profiling in identification of genetic modification in foods. Croat. Chem. Acta 2011, 84, 375-382.
40. Sazelova, P.; Kasicka, V.; Ibañez, E.; Cifuentes, A. Extraction and separation of water-soluble proteins from bacillus thuringiensis-transgenic and non-transgenic maize species by CZE. J. Sep. Sci. 2009, 32, 3801-3808.
41. Sazelova, P.; Kasicka, V.; Leon, C.; Ibañez, E.; Cifuentes, A. Capillary electrophoretic profiling of tryptic digests of water soluble proteins from bacillus thuringiensis-transgenic and nontransgenic maize species. Food Chem. 2012, 134, 1607-1615.
42. Simó, C.; Dominguez-Vega, E.; Luisa Marina, M.; Concepcion Garcia, M.; Dinelli, G.; Cifuentes, A. CE-TOF MS analysis of complex protein hydrolyzates from genetically modified soybeans—A tool for foodomics. Electrophoresis 2010, 31, 1175-1183.
43. Leon, C.; Rodriguez-Meizoso, I.; Lucio, M.; Garcia-Cañas, V.; Ibañez, E.; Schmitt-Kopplin, P.; Cifuentes, A. Metabolomics of transgenic maize combining fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extraction. J. Chromatogr. 2009, 1216, 7314-7323.
44. Sato, D.; Akashi, H.; Sugimoto, M.; Tomita, M.; Soga, T. Metabolomic profiling of the response of susceptible and resistant soybean strains to foxglove aphid, aulacorthum solani kaltenbach. J. Chromatogr. 2013, 925, 95-103.
45. Suzuki, Y.; Fujimori, T.; Kanno, K.; Sasaki, A.; Ohashi, Y.; Makino, A. Metabolome analysis of photosynthesis and the related primary metabolites in the leaves of transgenic rice plants with increased or decreased rubisco content. Plant Cell Environ. 2012, 35, 1369-1379.
46. Giuffrida, A.; Leon, C.; Garcia-Canas, V.; Cucinotta, V.; Cifuentes, A. Modified cyclodextrins for fast and sensitive chiral-capillary electrophoresis-mass spectrometry. Electrophoresis 2009, 30, 1734-1742.
47. Blaschke, G.; Chankvetadze, B. Enantiomer separation of drugs by capillary electromigration techniques. J. Chromatogr. 2000, 875, 3-25.
48. Giuffrida, A.; Tabera, L.; González, R.; Cucinotta, V.; Cifuentes, A. Chiral analysis of amino acids from conventional and transgenic yeasts. J. Chromatogr. 2008, 875, 243-247.
49. Breadmore, M.C. Capillary and microchip electrophoresis: Challenging the common conceptions. J. Chromatogr. 2012, 1221, 42-55.
50. Nagl, S.; Schulze, P.; Ludwig, M.; Belder, D. Progress in microchip enantioseparations. Electrophoresis 2009, 30, 2765-2772.
51. Dolnik, V.; Liu, S.R.; Jovanovich, S. Capillary electrophoresis on microchip. Electrophoresis 2000, 21, 41-54.
52. Dolnik, V.; Liu, S.R. Applications of capillary electrophoresis on microchip. J. Sep. Sci. 2005, 28, 1994-2009.
53. Burrell, A.; Foy, C.; Burns, M. Applicability of three alternative instruments for food authenticity analysis: GMO identification. Biotechnol. Res. Int. 2011, 2011, 838232.
54. Refinement of GMO screening methods by combining existing multiplex PCR approaches with lab-on-a-chip capillary electrophoresis endpoint detection. Available online: http://www.foodbase.org. uk//admintools/reportdocuments/300-1-525_G03022_FSA_GMO_Project_Final_Report.pdf (accessed on 15 October 2014).
55. Kluga, L.; Folloni, S.; Kagkli, D.M.; Bogni, A.; Foti, N.; Savini, C.; Mazzara, M.; van den Eede, G.; van den Bulcke, M. Combinatory SYBR® green real-time PCR screening approach for tracing materials derived from genetically modified rice. Food Anal. Methods 2013, 6, 361-369.
56. Pobozy, E.; Filaber, M.; Koc, A.; Garcia-Reyes, J.F. Application of capillary electrophoretic chips in protein profiling of plant extracts for identification of genetic modifications of maize. Electrophoresis 2013, 34, 2740-2753.
57. Nan, H.; Lee, S.-W.; Kang, S.H. Fast screening of rice knockout mutants by multi-channel microchip electrophoresis. Talanta 2012, 97, 249-255.