Dandelion PPO-1/PPO-2 domain-swaps: The C-terminal domain modulates the pH optimum and the linker affects SDS-mediated activation and stability

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1854, Issue 2, 2015, Pages 178-186

  Leufken, Christine M ^a   Moerschbacher, Bruno M ^a   Dirks Hofmeister, Mareike E ^a,b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Activation mechanism; Domain swap; Polyphenol oxidase; SDS activation; SDS stability; Three domain structure

Indexed keywords

BUFFER; CATECHOL OXIDASE; COPPER PROTEIN; DODECYL SULFATE SODIUM; ENZYME VARIANT; POLYPHENOL OXIDASE 1; POLYPHENOL OXIDASE 2; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DANDELION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STABILITY; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; SPECTROPHOTOMETRY; WILD TYPE; BINDING SITE; CHEMISTRY; DRUG EFFECTS; ENZYMOLOGY; KINETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

TARAXACUM OFFICINALE;

BINDING SITES; CATECHOL OXIDASE; ENZYME ACTIVATION; HYDROGEN-ION CONCENTRATION; KINETICS; PROTEIN STRUCTURE, TERTIARY; SODIUM DODECYL SULFATE; TARAXACUM;

EID: 84919754044     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2014.11.007     Document Type: Article

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