Isobutyrylshikonin inhibits lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in BV2 microglial cells by suppressing the PI3K/Akt-mediated nuclear transcription factor-κB pathway

Nutrition Research

Volumn 34, Issue 12, 2014, Pages 1111-1119

  Jayasooriya, Rajapaksha Gedara Prasad Tharanga ^a   Lee, Kyoung Tae ^b   Kang, Chang Hee ^a   Dilshara, Matharage Gayani ^a   Lee, Hak Ju ^b   Choi, Yung Hyun ^c   Choi, Il Whan ^d   Kim, Gi Young ^a  

^a Jeju National University   (South Korea)

^b Korea Forest Research Institute   (South Korea)

^c Dong Eui University   (South Korea)

^d Inje University College of Medicine   (South Korea)

Author keywords

Isobutyrylshikonin; Nitric oxide; Nuclear factor B; PI3K Akt; Prostaglandin E2

Indexed keywords

ANTIINFLAMMATORY AGENT; CYCLOOXYGENASE 2; I KAPPA B ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; ISOBUTYRYLSHIKONIN; NITRIC OXIDE; PHOSPHATIDYLINOSITOL 3 KINASE; PROSTAGLANDIN E2; PROTEIN KINASE B; PROTEIN P50; SYNAPTOTAGMIN I; UNCLASSIFIED DRUG; ANTIOXIDANT; AUTACOID; LIPOPOLYSACCHARIDE; NAPHTHOQUINONE; NOS2 PROTEIN, HUMAN; PLANT EXTRACT; PYRROLIDINE DERIVATIVE; PYRROLIDINE DITHIOCARBAMIC ACID; THIOCARBAMIC ACID DERIVATIVE;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; COX 2 GENE; DNA BINDING; DOWN REGULATION; DRUG IDENTIFICATION; DRUG STRUCTURE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REPRESSION; GENE; GENE EXPRESSION; INOS GENE; MICROGLIA; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; BIOSYNTHESIS; CELL LINE; CENTRAL NERVOUS SYSTEM DISEASES; CHEMICALLY INDUCED; CHEMISTRY; DRUG EFFECTS; INFLAMMATION; ISOLATION AND PURIFICATION; LITHOSPERMUM; MACROPHAGE; METABOLISM; PHOSPHORYLATION; PHYTOTHERAPY;

ANTIOXIDANTS; CELL LINE; CENTRAL NERVOUS SYSTEM DISEASES; CYCLOOXYGENASE 2; DINOPROSTONE; DOWN-REGULATION; INFLAMMATION; INFLAMMATION MEDIATORS; LIPOPOLYSACCHARIDES; LITHOSPERMUM; MACROPHAGES; MICROGLIA; NAPHTHOQUINONES; NF-KAPPA B; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE II; PHOSPHATIDYLINOSITOL 3-KINASES; PHOSPHORYLATION; PHYTOTHERAPY; PLANT EXTRACTS; PROTO-ONCOGENE PROTEINS C-AKT; PYRROLIDINES; SIGNAL TRANSDUCTION; THIOCARBAMATES;

EID: 84919492287     PISSN: 02715317     EISSN: 18790739     Source Type: Journal    
DOI: 10.1016/j.nutres.2014.10.002     Document Type: Article

References (27)

1. Ferret P.J., Soum E., Negre O., Fradelizi D. Auto-protective redox buffering systems in stimulated macrophages. BMC Immunol 2002, 3:3.
2. Olson J.K., Miller S.D. Microglia initiate central nervous system innate and adaptive immune responses through multiple TLRs. J Immunol 2004, 173:3916-3924.
3. Prediger R.D. Effects of caffeine in Parkinson's disease: from neuroprotection to the management of motor and non-motor symptoms. J Alzheimers Dis 2010, 20:S205-S220.
4. Xu K., Xu Y.H., Chen J.F., Schwarzschild M.A. Neuroprotection by caffeine: time course and role of its metabolites in the MPTP model of Parkinson's disease. Neuroscience 2010, 167:475-481.
5. Connelly L., Palacios-Callender M., Ameixa C., Moncada S., Hobbs A.J. Biphasic regulation of NF-κB activity underlies the pro- and anti-inflammatory actions of nitric oxide. J Immunol 2001, 166:3873-3881.
6. Baldwin A.S. Series introduction: the transcription factor NF-κB and human disease. J Clin Invest 2001, 107:3-6.
7. Sarada S., Himadri P., Mishra C., Geetali P., Ram M.S., Ilavazhagan G. Role of oxidative stress and NFκB in hypoxia-induced pulmonary edema. Exp Biol Med 2008, 233:1088-1098.
8. Weitz-Schmidt G. Statins as anti-inflammatory agents. Trends Pharmacol Sci 2002, 23:482-486.
9. Saponaro C., Cianciulli A., Calvello R., Dragone T., Lacobazzi F., Panaro M.A. The PI3K/Akt pathway is required for LPS activation of microglial cells. Immunopharmacol Immunotoxicol 2012, 34:858-865.
10. -/- mouse brain. Am J Pathol 2005, 167:1081-1092.
11. Park J.Y., Lee S., Han S., Kim H.M., Lee J.M., Lee S. A paraben derivative from the seeds of Lithospermum erythrorhizon. J Korean Soc Appl Biol Chem 2009, 52:643-645.
12. Rajasekar S., Park D.J., Park C., Park S., Park Y.H., Kim S.T., et al. In vitro and in vivo anticancer effects of Lithospermum erythrorhizon extract on B16F10 murine melanoma. J Ethnopharmacol 2012, 144:335-345.
13. Andújar I., Recio M.C., Bacelli T., Giner R.M., Ríos J.L. Shikonin reduces oedema induced by phorbol ester by interfering with IκBα degradation thus inhibiting translocation of NF-κB to the nucleus. Br J Pharmacol 2010, 160:376-388.
14. Nam K.N., Son M.S., Park J.H., Lee E.H. Shikonins attenuate microglial inflammatory responses by inhibition of ERK, Akt, and NF-κB: neuroprotective implications. Neuropharmacology 2008, 55:819-825.
15. Staniforth V., Wang S.Y., Shyur L.F., Yang N.S. Shikonins, phytocompounds for Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo. J Biol Chem 2004, 279:5877-5885.
16. MacMicking J., Xie Q.W., Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol 1997, 15:323-350.
17. Li M., Dai F.R., Du X.P., Yang Q.D., Chen Y. Neuroprotection by silencing iNOS expression in a 6-OHDA model of Parkinson's disease. J Mol Neurosci 2012, 48:225-233.
18. Woodling N.S., Wang Q., Priyam P.G., Larkin P., Shi J., Johansson J.U., et al. Suppression of Alzheimer-associated inflammation by microglial prostaglandin-E2 EP4 receptor signaling. J Neurosci 2014, 34:5882-5894.
19. Kumar A., Chen S.H., Kadiiska M.B., Hong J.S., Zielonka J., Kalyanaraman B., et al. Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine microglial BV2 cells. Free Radic Biol Med 2014, 73:51-59.
20. Rubio-Perez J.M., Morillas-Ruiz J.M. A review: inflammatory process in Alzheimer's disease, role of cytokines. ScientificWorldJournal 2012, 2012:756357.
21. Tak P.P., Firestein G.S. NF-κB: a key role in inflammatory diseases. J Clin Invest 2001, 107:7-11.
22. Bharti A.C., Donato N., Singh S., Aggarwal B.B. Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor-κB and IκBα kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis. Blood 2003, 101:1053-1062.
23. Dhar A., Young M.R., Colburn N.H. The role of AP-1, NF-κB and ROS/NOS in skin carcinogenesis: the JB6 model is predictive. Mol Cell Biochem 2002, 234-235:185-193.
24. Frakes A.E., Ferraiuolo L., Haidet-Phillips A.M., Schmelzer L., Braun L., Miranda C.J., et al. Microglia induce motor neuron death via the classic NF-κB pathway in amyotrophic lateral sclerosis. Neuron 2014, 81:1009-1023.
25. Li X., Tupper J.C., Bannerman D.D., Winn R.K., Rhodes C.J., Harlan J.M. Phosphoinositide 3 kinase mediates Toll-like receptor 4-induced activation of NF-κB in endothelial cells. Infect Immun 2003, 71:4414-4420.
26. Liu X., Shah A., Gangwani M.R., Silverstein P.S., Fu M., Kumar A. HIV-1 Nef induces CCL5 production in astrocytes through p38-MAPK and PI3K/Akt pathway and utilizes NF-κB, CEBP and AP-1 transcription factors. Sci Rep 2014, 4:4450.
27. Bianchi R., Giambanco I., Donato R. S100B/RAGE-dependent activation of microglia via NF-κB and AP-1 co-regulation of COX-2 expression by S100B, IL-1β and TNF-α. Neurobiol Aging 2010, 4:665-677.