Stoichiometry and assembly of mTOR complexes revealed by single-molecule pulldown

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 50, 2014, Pages 17833-17838

  Jain, Ankur ^a,b   Arauz, Edwin ^a   Aggarwal, Vasudha ^a,b   Ikon, Nikita ^a   Chen, Jie ^a   Ha, Taekjip ^a,b,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c University of Illinois at Urbana Champaign   (United States)

Author keywords

MTOR; MTORC; Rapamycin; Single molecule; Stoichiometry

Indexed keywords

HOMODIMER; MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 1; MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 2; MONOMER; BACTERIAL PROTEIN; MECHANISTIC TARGET OF RAPAMYCIN COMPLEX 1; MULTIPROTEIN COMPLEX; PHOTOPROTEIN; RAPAMYCIN; RED FLUORESCENT PROTEIN; TARGET OF RAPAMYCIN KINASE; TOR COMPLEX 2; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; ASSAY; CELL ENERGY; CONTROLLED STUDY; DIMERIZATION; FLUORESCENCE MICROSCOPY; NUTRIENT AVAILABILITY; SIGNAL TRANSDUCTION; SINGLE MOLECULE PULLDOWN ASSAY; STOICHIOMETRY; CHEMICAL STRUCTURE; CHEMISTRY; HEK293 CELL LINE; HUMAN; IMMUNOPRECIPITATION; METABOLISM; WESTERN BLOTTING;

MAMMALIA; RAPTORES;

BACTERIAL PROTEINS; BLOTTING, WESTERN; DIMERIZATION; HEK293 CELLS; HUMANS; IMMUNOPRECIPITATION; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; MULTIPROTEIN COMPLEXES; SIROLIMUS; TOR SERINE-THREONINE KINASES;

EID: 84919346597     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1419425111     Document Type: Article

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