Volta potential phase plate for in-focus phase contrast transmission electron microscopy

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 44, 2014, Pages 15635-15640

  Danev, Radostin ^a   Buijsse, Bart ^b   Khoshouei, Maryam ^a   Plitzko, Jürgen M ^a,c   Baumeister, Wolfgang ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b Thermo Fisher Scientific   (United States)

^c UTRECHT UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; CARBON NANOTUBE; GOLD NANOPARTICLE; GRAPHENE; LIQUID NITROGEN;

ARTICLE; AUGER ELECTRON SPECTROSCOPY; BEAM INDUCED PHASE SHIFT; CELL MEMBRANE; CHEMICAL COMPOSITION; CHEMICAL REACTION; CHEMISTRY; CONTRAST TRANSFER FUNCTION; DEGRADATION; DIFFRACTION; ELECTRIC POTENTIAL; ELECTRON; ELECTRON BEAM; ELECTRON ENERGY LOSS SPECTROSCOPY; ELECTRON TOMOGRAPHY; FILM; FOURIER TRANSFORMATION; GAS; HEATING; IRRADIATION; LUMBRICUS TERRESTRIS; MICROTUBULE; NOISE; NONHUMAN; OSCILLATION; OXIDATION; PHASE CONTRAST MICROSCOPY; PHYSICS; RAMAN SPECTROMETRY; SPERMATOZOON TAIL; SURFACE PROPERTY; TEMPERATURE; TIME; TRANSMISSION ELECTRON MICROSCOPY; VACUUM; VELOCITY; VOLTA POTENTIAL PHASE PLATE; X RAY PHOTOELECTRON SPECTROSCOPY;

EID: 84914674933     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1418377111     Document Type: Article

References (24)

1. Nagayama K, Danev R (2008) Phase contrast electron microscopy: Development of thin-film phase plates and biological applications. Philos Trans R Soc Lond B Biol Sci 363(1500):2153-2162.
2. Walter A, et al. (2012) Practical aspects of Boersch phase contrast electron microscopy of biological specimens. Ultramicroscopy 116:62-72.
3. Frindt N, et al. (2014) In-focus electrostatic Zach phase plate imaging for transmission electron microscopy with tunable phase contrast of frozen hydrated biological samples. Microsc Microanal Canada 20(1):175-183.
4. Edgcombe CJ, et al. (2012) Characterisation of ferromagnetic rings for Zernike phase plates using the Aharonov-Bohm effect. Ultramicroscopy 120:78-85.
5. Müller H, et al. (2010) Design of an electron microscope phase plate using a focused continuous-wave laser. New J Phys 12:12.
6. Buijsse B, et al. (2011) Design of a hybrid double-sideband/single-sideband (schlieren) objective aperture suitable for electron microscopy. Ultramicroscopy 111(12):1688-1695.
7. Danev R, Nagayama K (2010) Phase plates for transmission electron microscopy. Methods Enzymol 481:343-369.
8. Glaeser RM (2013) Invited review article: Methods for imaging weak-phase objects in electron microscopy. Rev Sci Instrum 84(11):111101.
9. Dai W, et al. (2013) Visualizing virus assembly intermediates inside marine cyanobacteria. Nature 502(7473):707-710.
10. Rochat RH, et al. (2011) Seeing the portal in herpes simplex virus type 1 B capsids. J Virol 85(4):1871-1874.
11. Danev R, Glaeser RM, Nagayama K (2009) Practical factors affecting the performance of a thin-film phase plate for transmission electron microscopy. Ultramicroscopy 109(4):312-325.
12. Murata K, et al. (2010) Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions. Structure 18(8):903-912.
13. Fukuda Y, Nagayama K (2012) Zernike phase contrast cryo-electron tomography of whole mounted frozen cells. J Struct Biol 177(2):484-489.
14. Fukuda Y, Fukazawa Y, Danev R, Shigemoto R, Nagayama K (2009) Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens. J Struct Biol 168(3):476-484.
15. Yamaguchi M, Danev R, Nishiyama K, Sugawara K, Nagayama K (2008) Zernike phase contrast electron microscopy of ice-embedded influenza A virus. J Struct Biol 162(2): 271-276.
16. Danev R, Nagayama K (2011) Optimizing the phase shift and the cut-on periodicity of phase plates for TEM. Ultramicroscopy 111(8):1305-1315.
17. Danev R, Kanamaru S, Marko M, Nagayama K (2010) Zernike phase contrast cryoelectron tomography. J Struct Biol 171(2):174-181.
18. Johnson HM, Parsons DF (1973) Enhanced contrast in electron microscopy of unstained biological material. 3. In-focus phase contrast of large objects. J Microsc 98(1):1-17.
19. Unwin PN (1973) Phase contrast electron microscopy of biological materials. J Microsc 98(3):299-312.
20. Malac M, Beleggia M, Kawasaki M, Li P, Egerton RF (2012) Convenient contrast enhancement by a hole-free phase plate. Ultramicroscopy 118:77-89.
21. Danov K, Danev R, Nagayama K (2001) Electric charging of thin films measured using the contrast transfer function. Ultramicroscopy 87(1-2):45-54.
22. Downing KH, McCartney MR, Glaeser RM (2004) Experimental characterization and mitigation of specimen charging on thin films with one conducting layer. Microsc Microanal 10(6):783-789.
23. Kumar PV, Bernardi M, Grossman JC (2013) The impact of functionalization on the stability, work function, and photoluminescence of reduced graphene oxide. ACS Nano 7(2):1638-1645.
24. Ago H, et al. (1999) Work functions and surface functional groups of multiwall carbon nanotubes. J Phys Chem B 103(38):8116-8121.