In-focus electrostatic zach phase plate imaging for transmission electron microscopy with tunable phase contrast of frozen hydrated biological samples

Microscopy and Microanalysis

Volumn 20, Issue 1, 2014, Pages 175-183

  Frindt, Nicole ^a   Oster, Marco ^a   Hettler, Simon ^b   Gamm, Björn ^b   Dieterle, Levin ^c   Kowalsky, Wolfgang ^c,d   Gerthsen, Dagmar ^b   Schröder, Rasmus R ^a,d,e  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

^c TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^d INNOVATIONLAB   (Germany)

^e UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Charging; Contamination; Cryo samples; Cryo transmission electron microscopy; Electrostatic phase plate; Frozen hydrated biological samples; Phase contrast; Single sideband effect; Weak phase object

Indexed keywords

ACTIN; CATALASE;

ANIMAL; ARTICLE; CATTLE; ELECTRODE; EQUIPMENT; HISTOLOGY; METHODOLOGY; PHASE CONTRAST MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ACTINS; ANIMALS; CATALASE; CATTLE; ELECTRODES; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, PHASE-CONTRAST;

EID: 84896494544     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927613013901     Document Type: Article

References (25)

1. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. & Walter, P. (2008). Molecular Biology of the Cell, 5th ed. New York: Garland Science Taylor & Francis Group.
2. Boersch, H. (1947). Uber die Kontraste von Atomen im Elektronenmikroskop. Z Naturforschung 2a, 615-633.
3. Buijsse, B., Van Laarhoven, F.M.H.M., Schmid, A.K., Cambie, R., Cabrini, S., Jin, J. & Glaeser, R.M. (2011). Design of a hybrid double-sideband/ single-sideband (schlieren) objective aperture suitable for electron microscopy. Ultramicroscopy 111, 1688-1695.
4. Cambie, R., Downing, K.H., Typke, D., Glaeser, R.M. & Jin, J. (2007). Design of a microfabricated, two-electrode phasecontrast element suitable for electron microscopy. Ultramicroscopy 107, 329-339.
5. Danev, R. & Nagayama, K. (2001). Transmission electron microscopy with Zernike phase plate. Ultramicroscopy 88, 243-252.
6. Danev, R. & Nagayama, K. (2008). Single particle analysis based on Zernike phase contrast transmission electron microscopy. J Struct Biol 161, 211-218.
7. Danev, R. & Nagayama, K. (2011). Optimizing the phase shift and the cut-on periodicity of phase plates for TEM. Ultramicroscopy 111, 1305-1315.
8. Danev, R., Okawara, H., Usuda, N., Kametani, K. & Nagayama, K. (2002). A novel phase-contrast transmission electron microscopy producing high-contrast topographic images of weak objects. J Biol Phys 28, 627-635.
9. Dubochet, J., Lepault, J., Freeman, R., Berriman, J.A. & Homo, J.C. (1982). Electron microscopy of frozen water and aqueous solutions. J Microsc 128, 219-237.
10. Frindt, N. (2013). Development and implementation of electrostatic Zach phase plates for phase contrast transmission electron microscopy. PhD thesis. Universität Heidelberg, Heidelberg, Germany.
11. Gamm, B., Dries, M., Schultheiss, K., Blank, H., Rosenauer, A., SchrOder, R.R. & Gerthsen, D. (2010). Object wave reconstruction by phase-plate transmission electron microscopy. Ultramicroscopy 110, 807-814.
12. Glaeser, R.M., Sassolini, S., Cambie, R., Jin, J., Cabrini, S., Schmid, A., Danev, R., Buijsse, B., Csencsits, R., Downing, K.H., Larson, D.M., Typke, D. & Han, B.G. (2013). Minimizing electrostatic charging of an aperture used to produce infocus phase contrast in the TEM. Ultramicroscopy 135, 6-15.
13. Hettler, S., Gamm, B., Dries, M., Frindt, N., SchrOder, R.R. & Gerthsen, D. (2012). Improving fabrication and application of Zach phase plates for phase-contrast transmission electron microscopy. Microsc Microanal 18, 1010-1015.
14. Holmes, K.C., Popp, D., Gebhard, W. & Kabsch, W. (1990). Atomic model of the actin filament. Nature 347, 44-49.
15. Majorovits, E., Barton, B., Schultheiss, K., Perez-Willard, F., Gerthsen, D. & SchrOder, R.R. (2007). Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate. Ultramicroscopy 107, 213-226.
16. Matsumoto, T. & Tonomura, A. (1996). The phase constancy of electron waves traveling through Boerschs electrostatic phase plate. Ultramicroscopy 63, 5-10.
17. Murata, K., Liu, X., Danev, R., Jakana, J., Schmid, M.F., King, J., Nagayama, K. & Chiu, W. (2010). Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and sub-nanometer resolutions. Structure 18, 903-912.
18. Nagayama, K. (2008). Development of phase plates for electron microscopes and their biological application. Eur Biophys J 37, 345-358.
19. Reimer, L. & Kohl, H. (2008). Transmission Electron Microscopy: Physics of Image Formation. New York: Springer Verlag.
20. Schultheiss, K., Perez-Willard, F., Barton, B., Gerthsen, D. & SchrOder, R.R. (2006). Fabrication of a Boersch phase plate for phase contrast imaging in a transmission electron microscope. Rev Sci Instrum 77, 033701.
21. Schultheiss, K., Zach, J., Gamm, B., Dries, M., Frindt, N., SchrOder, R.R. & Gerthsen, D. (2010). New electrostatic phase plate for transmission electron microscopy and its application for wave-function reconstruction. Microsc Microanal 16, 536-537.
22. Vitos, L., Ruban, A.V., Skriver, H.L. & Kollar, J. (1998). The surface energy of metals. Surf Sci 411, 186-202.
23. Walker, M., Nordquist, C., Czaplewski, D., Patrizi, G., McGruer, N. & Krim, J. (2010). Impact of in situ oxygen plasma cleaning on the resistance of Ru and Au-Ru based rf microelectromechanical system contacts in vacuum. J Appl Phys 107, 084509.
24. Weber, L., Lehr, M. & Gmelin, E. (1996). Investigation of the transport properties of gold point contacts. Physica B Condens Matter 217, 181-192.
25. Zach, J. (2008). Phase plate, image producing method and electron microscope. PCT Patent WO/2008/061603; EPO Patent 1989723-A2, European Patent Office, Munich.