Zernike phase contrast electron microscopy of ice-embedded influenza A virus

Journal of Structural Biology

Volumn 162, Issue 2, 2008, Pages 271-276

  Yamaguchi, Masashi ^a   Danev, Radostin ^b   Nishiyama, Kiyoto ^c   Sugawara, Keishin ^c   Nagayama, Kuniaki ^b  

^a CHIBA UNIVERSITY   (Japan)

^b OKAZAKI INSTITUTE FOR INTEGRATIVE BIOSCIENCE   (Japan)

^c Chemo Sero Therapeutic Research Institute   (Japan)

Author keywords

Glycoprotein spikes; Influenza A virus; Phase contrast; Rapid freezing; Ribonucleoprotein complex; Transmission electron microscopy

Indexed keywords

GLYCOPROTEIN; ICE; RIBONUCLEOPROTEIN;

ANALYTIC METHOD; ARTICLE; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPY; EMBEDDING; FREEZING; INFLUENZA VIRUS A; LIPID BILAYER; NONHUMAN; PHASE CONTRAST MICROSCOPY; PRIORITY JOURNAL; PROTEIN STRUCTURE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; VIRION; VIRUS ENVELOPE; VIRUS MORPHOLOGY; VIRUS PARTICLE; ZERNIKE PHASE CONTRAST ELECTRON MICROSCOPY;

CRYOELECTRON MICROSCOPY; GLYCOPROTEINS; ICE; INFLUENZA A VIRUS; LIPID BILAYERS; MICROSCOPY, PHASE-CONTRAST; VIRAL ENVELOPE PROTEINS; VIRION;

INFLUENZA A VIRUS;

EID: 42649085376     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2008.01.009     Document Type: Article

References (18)

1. Adrian M., Dubochet J., Lepault J., and McDowell A. Cryo-electron microscopy of viruses. Nature 308 (1984) 32-36
2. Booy F.P., Ruigrok R.W.H., and van Bruggen E.F.J. Electron microscopy of influenza virus. A comparison of negatively stained and ice-embedded particles. J. Mol. Biol. 184 (1985) 667-676
3. Danev R., and Nagayama K. Transmission electron microscopy with Zernike phase plate. Ultramicroscopy 88 (2001) 243-252
4. Danev R., and Nagayama K. Single particle analysis based on Zernike phase contrast transmission electron microscopy. J. Struct. Biol. 161 (2008) 211-218
5. Fujiyoshi Y., Kume N.P., Sakata K., and Sato S.B. Fine structure of influenza A virus observed by electron cryo-microscopy. EMBO J. 13 (1994) 318-326
6. Harris A., Cardone G., Winkler D.C., Heymann J.B., Brecher M., White J.M., and Steven A.C. Influenza virus pleiomorphy characterized by cryoelectron tomography. Proc. Natl. Acad. Sci. USA 103 (2006) 19123-19127
7. Jäälinoja H.T., Huiskonen J.T., and Butcher S.J. Electron cryomicroscopy comparison of the architectures of the enveloped bacteriophages ø6 and ø8. Structure 15 (2007) 157-167
8. Kaneko Y., Danev R., Nagayama K., and Nakamoto H. Intact carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy. J. Bacteriol. 188 (2006) 805-808
9. Kaneko Y., Danev R., Nitta K., and Nagayama K. In vivo subcellular ultrastructures recognized with Hilbert differential contrast transmission electron microscopy. J. Electron Microsc. 54 (2005) 79-84
10. Krug R.M. The Influenza Viruses (1989), Plenum Press, New York
11. Lamb R.A., and Krug R.M. Orthomyxoviridae: the viruses and their replication. In: Knipe D.M., and Howley P.M. (Eds). Fields Virology. fourth ed. (2001), Lippinncott Williams & Wilkins, Philadelphia, PA 1487-1531
12. Lee K.K., and Johnson J.E. Complementary approaches to structure determination of icosahedral viruses. Curr. Opin. Struct. Biol. 13 (2003) 558-569
13. Lučić V., Förster F., and Baumeister W. Structural studies by electron tomography: from cells to molecules. Annu. Rev. Biochem. 74 (2005) 833-865
14. Nagayama K. Phase contrast enhancement with phase plates in electron microscopy. Adv. Imag. Elect. Phys. 138 (2005) 69-146
15. Nayak D.P., Hui E.K.-W., and Barman S. Assembly and budding of influenza virus. Virus Res. 106 (2004) 147-165
16. Noda T., Sagara H., Yen A., Takada A., Kida H., Cheng R.H., and Kawaoka Y. Architecture of ribonucleoprotein complexes in influenza A virus particles. Nature 439 (2006) 490-492
17. Ruigrok R.W.H., Andree P.J., van Huysduynen R.A.M.H., and Mellema J.E. Characterization of three highly purified influenza virus strains by electron microscopy. J. Gen. Virol. 65 (1984) 799-802
18. Yamaguchi M., Hirano T., Hirokawa H., Sugawara K., Mizokami H., and Matsubara K. Cryo-electron microscopy of hepatitis B virus core particles produced by transformed yeast: comparison with negative staining and ultrathin sectioning. J. Electron Microsc. 37 (1988) 337-341