Characteristic changes in microbial community composition and expression of innate immune genes in acute appendicitis

Innate Immunity

Volumn 21, Issue 1, 2015, Pages 30-41

  Arlt, Alexander ^a   Bharti, Richa ^a   Ilves, Imre ^b   Häsler, Robert ^a   Miettinen, Pekka ^b   Paajanen, Hannu ^b   Brunke, Gabriele ^a   Ellrichmann, Mark ^a   Rehman, Ateequr ^a,c   Hauser, Charlotte ^a   Egberts, Jan Hendrik ^a   Ott, Stephan J ^a,b   Schreiber, Stefan ^a,b   Rosenstiel, Philip ^a   Herzig, Karl Heinz ^a,b,d  

^a UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

^b KUOPIO UNIVERSITY HOSPITAL   (Finland)

^c UNIVERSITY OF FREIBURG   (Germany)

^d UNIVERSITY OF OULU   (Finland)

Author keywords

Antimicrobial peptides; appendicitis; defensins; inflammation; innate immunity; microbial community

Indexed keywords

ALPHA DEFENSIN; BETA DEFENSIN; BETA DEFENSIN 1; BETA DEFENSIN 2; BETA DEFENSIN 3; COMPLEMENTARY DNA; DNA 16S; INTERLEUKIN 10; INTERLEUKIN 1BETA; INTERLEUKIN 6; INTERLEUKIN 8; RNA 16S; TOLL LIKE RECEPTOR 1; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 3; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 6; TOLL LIKE RECEPTOR 8; TOLL LIKE RECEPTOR 9; DEFENSIN; MESSENGER RNA; PEPTIDE;

ACUTE APPENDICITIS; ADULT; ARTICLE; CLINICAL ARTICLE; COLON MUCOSA; CONTROLLED STUDY; DNA SYNTHESIS; DOWN REGULATION; FEMALE; GENE EXPRESSION; HUMAN; HUMAN TISSUE; ILEUM MUCOSA; IMMUNE RESPONSE; INNATE IMMUNITY; MALE; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; PARABACTEROIDES DISTASONIS; PATHOGENESIS; PATHOPHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; UPREGULATION; ACUTE DISEASE; ADOLESCENT; APPENDICITIS; BACTERIUM; BIOSYNTHESIS; CLASSIFICATION; DNA MICROARRAY; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; IN VITRO STUDY; MICROBIOLOGY; MIDDLE AGED; YOUNG ADULT;

ACUTE DISEASE; ADOLESCENT; ADULT; APPENDICITIS; BACTERIA; DEFENSINS; DNA, COMPLEMENTARY; FEMALE; GENE EXPRESSION REGULATION; HUMANS; IMMUNITY, INNATE; IN VITRO TECHNIQUES; MALE; MIDDLE AGED; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PEPTIDES; RNA, MESSENGER; RNA, RIBOSOMAL, 16S; YOUNG ADULT;

EID: 84914166064     PISSN: 17534259     EISSN: 17534267     Source Type: Journal    
DOI: 10.1177/1753425913515033     Document Type: Article

References (51)

1. Miettinen P, Pasanen P, Lahtinen J, Alhava E. Acute abdominal pain in adults. Ann Chir Gynaecol. 1996 ; 85: 5-9
2. Liu K, Fogg L. Use of antibiotics alone for treatment of uncomplicated acute appendicitis: a systematic review and meta-analysis. Surgery. 2011 ; 150: 673-683
3. Lamps LW. Appendicitis and infections of the appendix. Semin Diagn Pathol. 2004 ; 21: 86-97
4. Yamamoto T, Umegae S, Kitagawa T, Matsumoto K. Intraperitoneal cytokine productions and their relationship to peritoneal sepsis and systemic inflammatory markers in patients with inflammatory bowel disease. Dis Colon Rectum. 2005 ; 48: 1005-1015
5. Peterfi Z, Kovacs K, Antal A, Kocsis B. Anti-lipopolysaccharide antibodies in acute appendicitis detected by ELISA. APMIS. 2006 ; 114: 265-269
6. Swidsinski A, Dorffel Y, Loening-Baucke V, et al. Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum. Gut. 2011 ; 60: 34-40
7. Ott SJ, Musfeldt M, Wenderoth DF, et al. Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. Gut. 2004 ; 53: 685-693
8. Trinchieri G, Sher A. Cooperation of Toll-like receptor signals in innate immune defence. Nat Rev Immunol. 2007 ; 7: 179-190
9. Rosenstiel P, Till A, Schreiber S. NOD-like receptors and human diseases. Microbes Infect. 2007 ; 9: 648-657
10. Otte JM, Boser S, Brunke G, et al. Expression of keratinocyte growth factor and its receptor in adaptive changes of ileorectal pouch mucosa. Scand J Gastroenterol. 2005 ; 40: 1066-1075
11. Otte JM, Kiehne K, Herzig KH. Antimicrobial peptides in innate immunity of the human intestine. J Gastroenterol. 2003 ; 38: 717-726
12. Wehkamp J, Fellermann K, Herrlinger KR, et al. Mechanisms of disease: defensins in gastrointestinal diseases. Nat Clin Pract Gastroenterol Hepatol. 2005 ; 2: 406-415
13. Steubesand N, Kiehne K, Brunke G, et al. The expression of the beta-defensins hBD-2 and hBD-3 is differentially regulated by NF-kappaB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis. BMC Immunol. 2009 ; 10: 36-36
14. Pahl R, Brunke G, Steubesand N, et al. IL-1{beta} and ADAM17 are central regulators of {beta}-defensin expression in Candida esophagitis. Am J Physiol Gastrointest Liver Physiol. 2011 ; 300: G547 - G553
15. Kiehne K, Brunke G, Wegner F, et al. Defensin expression in chronic pouchitis in patients with ulcerative colitis or familial adenomatous polyposis coli. World J Gastroenterol. 2006 ; 12: 1056-1062
16. Kiehne K, Fincke A, Brunke G, et al. Antimicrobial peptides in chronic anal fistula epithelium. Scand J Gastroenterol. 2007 ; 42: 1063-1069
17. Arlt A, Rosenstiel P, Kruse ML, et al. IEX-1 directly interferes with RelA/p65 dependent transactivation and regulation of apoptosis. Biochim Biophys Acta. 2008 ; 1783: 941-952
18. Laudien M, Hasler R, Wohlers J, et al. Molecular signatures of a disturbed nasal barrier function in the primary tissue of Wegener's granulomatosis. Mucosal Immunol. 2011 ; 4: 564-573
19. Hashimoto T, Perlot T, Rehman A, et al. ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. Nature. 2012 ; 487: 477-481
20. Rehman A, Sina C, Gavrilova O, et al. Nod2 is essential for temporal development of intestinal microbial communities. Gut. 2011 ; 60: 1262-1354
21. Pruesse E, Quast C, Knittel K, et al. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 2007 ; 35: 7188-7196
22. Schloss PD, Westcott SL, Ryabin T, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009 ; 75: 7537-7541
23. Edgar RC, Haas BJ, Clemente JC, et al. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. 2011 ; 27: 2194-2200
24. Wooley JC, Godzik A, Friedberg I. A primer on metagenomics. PLoS Comput Biol. 2010 ; 6: e1000667 - e1000667
25. Musso G, Gambino R, Cassader M. Interactions between gut microbiota and host metabolism predisposing to obesity and diabetes. Annu Rev Med. 2011 ; 62: 361-380
26. Lamps LW. Infectious causes of appendicitis. Infect Dis Clin North Am. 2010 ; 24: 995-1018
27. Wehkamp J, Stange EF. Paneth's disease. J Crohns Colitis. 2010 ; 4: 523-531
28. Salzman NH. Paneth cell defensins and the regulation of the microbiome: detente at mucosal surfaces. Gut Microbes. 2010 ; 1: 40140-40146
29. Soehnlein O, Kai-Larsen Y, Frithiof R, et al. Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages. J Clin Invest. 2008 ; 118: 3491-3502
30. Chakraborty K, Ghosh S, Koley H, et al. Bacterial exotoxins downregulate cathelicidin (hCAP-18/LL-37) and human beta-defensin 1 (HBD-1) expression in the intestinal epithelial cells. Cell Microbiol. 2008 ; 10: 2520-2537
31. Zaalouk TK, Bajaj-Elliott M, George JT, McDonald V. Differential regulation of beta-defensin gene expression during Cryptosporidium parvum infection. Infect Immun. 2004 ; 72: 2772-2779
32. Schroeder BO, Wu Z, Nuding S, et al. Reduction of disulphide bonds unmasks potent antimicrobial activity of human beta-defensin 1. Nature. 2011 ; 469: 419-423
33. Peyrin-Biroulet L, Beisner J, Wang G, et al. Peroxisome proliferator-activated receptor gamma activation is required for maintenance of innate antimicrobial immunity in the colon. Proc Natl Acad Sci U S A. 2010 ; 107: 8772-8777
34. Kaplan GG, Pedersen BV, Andersson RE, et al. The risk of developing Crohn's disease after an appendectomy: a population-based cohort study in Sweden and Denmark. Gut. 2007 ; 56: 1387-1392
35. Kota S, Sabbah A, Chang TH, et al. Role of human beta-defensin-2 during tumor necrosis factor-alpha/NF-kappaB-mediated innate antiviral response against human respiratory syncytial virus. J Biol Chem. 2008 ; 283: 22417-22429
36. Chen K, Huang J, Gong W, et al. Toll-like receptors in inflammation, infection and cancer. Int Immunopharmacol. 2007 ; 7: 1271-1285
37. Sumikawa Y, Asada H, Hoshino K, et al. Induction of beta-defensin 3 in keratinocytes stimulated by bacterial lipopeptides through toll-like receptor 2. Microbes Infect. 2006 ; 8: 1513-1521
38. Gross O, Poeck H, Bscheider M, et al. Syk kinase signalling couples to the Nlrp3 inflammasome for anti-fungal host defence. Nature. 2009 ; 459: 433-436
39. Rivera-Chavez FA, Peters-Hybki DL, Barber RC, et al. Innate immunity genes influence the severity of acute appendicitis. Ann Surg. 2004 ; 240: 269-277
40. Rivera-Chavez FA, Wheeler H, Lindberg G, et al. Regional and systemic cytokine responses to acute inflammation of the vermiform appendix. Ann Surg. 2003 ; 237: 408-416
41. Alexopoulou L, Thomas V, Schnare M, et al. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 2002 ; 8: 878-884
42. Funderburg NT, Jadlowsky JK, Lederman MM, et al. The Toll-like receptor 1/2 agonists Pam(3) CSK(4) and human beta-defensin-3 differentially induce interleukin-10 and nuclear factor-kappaB signalling patterns in human monocytes. Immunology. 2011 ; 134: 151-160
43. Redfern RL, Reins RY, McDermott AM. Toll-like receptor activation modulates antimicrobial peptide expression by ocular surface cells. Exp Eye Res. 2011 ; 92: 209-220
44. Heil F, Hemmi H, Hochrein H, et al. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 2004 ; 303: 1526-1529
45. Cervantes JL, Dunham-Ems SM, La Vake CJ, et al. Phagosomal signaling by Borrelia burgdorferi in human monocytes involves Toll-like receptor (TLR) 2 and TLR8 cooperativity and TLR8-mediated induction of IFN-beta. Proc Natl Acad Sci U S A. 2011 ; 108: 3683-3688
46. Lipinski S, Till A, Sina C, et al. DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses. J Cell Sci. 2009 ; 122: 3522-3530
47. Voss E, Wehkamp J, Wehkamp K, et al. NOD2/CARD15 mediates induction of the antimicrobial peptide human beta-defensin-2. J Biol Chem. 2006 ; 281: 2005-2011
48. Simms LA, Doecke JD, Walsh MD, et al. Reduced alpha-defensin expression is associated with inflammation and not NOD2 mutation status in ileal Crohn's disease. Gut. 2008 ; 57: 903-910
49. Guinane CM, Tadrous A, Fouhy F, et al. Microbial composition of human appendices from patients following appendectomy. mBio. 2013 ;: 4-4
50. Ott SJ, Kuhbacher T, Musfeldt M, et al. Fungi and inflammatory bowel diseases: Alterations of composition and diversity. Scand J Gastroenterol. 2008 ; 43: 831-841
51. Sjogren YM, Tomicic S, Lundberg A, et al. Influence of early gut microbiota on the maturation of childhood mucosal and systemic immune responses. Clin Exp Allergy. 2009 ; 39: 1842-1851