Glycosylation and disulfide bond analysis of transiently and stably expressed clade C HIV-1 gp140 trimers in 293T cells identifies disulfide heterogeneity present in both proteins and differences in o -linked glycosylation

Journal of Proteome Research

Volumn 13, Issue 9, 2014, Pages 4012-4027

  Go, Eden P ^a   Hua, David ^a   Desaire, Heather ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

disulfide bond; Envelope protein; glycopeptide; glycosylation; glycosylation site occupancy; HIV 1; immunogens; mass spectrometry; protein expression

Indexed keywords

DISULFIDE; GLYCOPROTEIN GP 140; GLYCOPROTEIN; GP140 ENVELOPE PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; VIRUS ENVELOPE PROTEIN;

ANALYTIC METHOD; ARTICLE; CLADISTICS; COLLISIONALLY ACTIVATED DISSOCIATION; CONTROLLED STUDY; DISULFIDE BOND; ELECTRON TRANSFER DISSOCIATION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; TRANSIENT TRANSFECTION; AMINO ACID SEQUENCE; CHEMISTRY; GLYCOSYLATION; HEK293 CELL LINE; HUMAN; MASS SPECTROMETRY; MOLECULAR GENETICS; PROCEDURES; PROTEOMICS;

HUMAN IMMUNODEFICIENCY VIRUS 1;

AMINO ACID SEQUENCE; DISULFIDES; ENV GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; GLYCOPROTEINS; GLYCOSYLATION; HEK293 CELLS; HIV-1; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEOMICS;

EID: 84914109422     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr5003643     Document Type: Article

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