Gene editing using ssODNs with engineered endonucleases

Methods in Molecular Biology

Volumn 1239, Issue , 2015, Pages 251-265

  Chen, Fuqiang ^a   Pruett Miller, Shondra M ^b   Davis, Gregory D ^a  

^a Sigma Aldrich Co   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

cas9; CRISPR; Double stranded break; Engineered endonucleases; Homology directed repair; Single stranded oligodeoxynucleotides; ssODNs; ZFN; Zinc finger nuclease

Indexed keywords

ENDONUCLEASE; GENOMIC DNA; OLIGODEOXYNUCLEOTIDE; SINGLE STRANDED OLIGODEOXYNUCLEOTIDE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE; OLIGODEOXYRIBONUCLEOTIDE; RIBOSOMAL PROTEIN S6 KINASE, 90KDA, POLYPEPTIDE 3; S6 KINASE; SINGLE STRANDED DNA;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CODON; CONTROLLED STUDY; DOUBLE STRANDED DNA BREAK; GENE DELETION; GENE EDITING; GENE INSERTION; GENETIC PROCEDURES; HUMAN; HUMAN CELL; DNA END JOINING REPAIR; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE TARGETING; GENE VECTOR; GENETIC ENGINEERING; GENETIC LOAD; GENETIC TRANSFECTION; GENETICS; MOLECULAR CLONING; PROCEDURES; SINGLE CELL ANALYSIS;

CLONING, MOLECULAR; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; DNA, SINGLE-STRANDED; ENDONUCLEASES; FLOW CYTOMETRY; GENE EXPRESSION; GENE TARGETING; GENETIC ENGINEERING; GENETIC LOAD; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; OLIGODEOXYRIBONUCLEOTIDES; RIBOSOMAL PROTEIN S6 KINASES, 90-KDA; SINGLE-CELL ANALYSIS; TRANSFECTION;

EID: 84911885395     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1862-1_14     Document Type: Article

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