Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1

Journal of the American Chemical Society

Volumn 136, Issue 44, 2014, Pages 15577-15583

  Schmied, Wolfgang H ^a   Elsässer, Simon J ^a   Uttamapinant, Chayasith ^a   Chin, Jason W ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIAN CELLS; MULTI-SITE; SYNTHETASES; UNNATURAL AMINO ACIDS;

AMINO ACID; AMINO ACID TRANSFER RNA LIGASE; ERF1 PROTEIN; MUTANT PROTEIN; PYRROLYSYL TRNA SYNTHETASE; RECOMBINANT PROTEIN; TRANSFER RNA; TRANSLATION TERMINATION FACTOR; UNCLASSIFIED DRUG; LYSINE TRANSFER RNA LIGASE; PYRROLE DERIVATIVE;

AMINO ACID METABOLISM; ANIMAL CELL; ARTICLE; GENE EXPRESSION SYSTEM; MAMMAL CELL; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN SYNTHESIS; REGULATORY MECHANISM; REPRODUCIBILITY; STOP CODON; TRANSLATION REGULATION;

AMINO ACIDS; LYSINE-TRNA LIGASE; PYRROLES; RNA, TRANSFER;

EID: 84908626417     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja5069728     Document Type: Article

References (41)

1. Lang, K.; Chin, J. W. Chem. Rev. 2014, 114, 4764
2. Kim, C. H.; Axup, J. Y.; Schultz, P. G. Curr. Opin Chem. Biol. 2013, 17, 412
3. Baker, A. S.; Deiters, A. ACS Chem. Biol. 2014, 9, 7
4. Davis, L.; Chin, J. W. Nat. Rev. Mol. Cell Biol. 2012, 13, 168
5. Chin, J. W. Annu. Rev. Biochem. 2014, 83, 379
6. Bertram, G.; Innes, S.; Minella, O.; Richardson, J.; Stansfield, I. Microbiology 2001, 147, 255
7. Jackson, R. J.; Hellen, C. U.; Pestova, T. V. Adv. Protein Chem. Struct. Biol. 2012, 86, 45
8. Dever, T. E.; Green, R. Cold Spring Harbor Perspect. Biol. 2012, 4, a013706
9. Scolnick, E.; Tompkins, R.; Caskey, T.; Nirenberg, M. Proc. Natl. Acad. Sci. U.S.A. 1968, 61, 768
10. Petry, S.; Brodersen, D. E.; Murphy, F. V. t.; Dunham, C. M.; Selmer, M.; Tarry, M. J.; Kelley, A. C.; Ramakrishnan, V. Cell 2005, 123, 1255
11. Konecki, D. S.; Aune, K. C.; Tate, W.; Caskey, C. T. J. Biol. Chem. 1977, 252, 4514
12. Wang, K.; Neumann, H.; Peak-Chew, S. Y.; Chin, J. W. Nat. Biotechnol. 2007, 25, 770
13. Mukai, T.; Hayashi, A.; Iraha, F.; Sato, A.; Ohtake, K.; Yokoyama, S.; Sakamoto, K. Nucleic Acids Res. 2010, 38, 8188
14. Johnson, D. B.; Xu, J.; Shen, Z.; Takimoto, J. K.; Schultz, M. D.; Schmitz, R. J.; Xiang, Z.; Ecker, J. R.; Briggs, S. P.; Wang, L. Nat. Chem. Biol. 2011, 7, 779
15. Lajoie, M. J.; Rovner, A. J.; Goodman, D. B.; Aerni, H. R.; Haimovich, A. D.; Kuznetsov, G.; Mercer, J. A.; Wang, H. H.; Carr, P. A.; Mosberg, J. A.; Rohland, N.; Schultz, P. G.; Jacobson, J. M.; Rinehart, J.; Church, G. M.; Isaacs, F. J. Science 2013, 342, 357
16. Wu, I. L.; Patterson, M. A.; Carpenter Desai, H. E.; Mehl, R. A.; Giorgi, G.; Conticello, V. P. ChemBioChem 2013, 14, 968
17. Mukai, T.; Kobayashi, T.; Hino, N.; Yanagisawa, T.; Sakamoto, K.; Yokoyama, S. Biochem. Biophys. Res. Commun. 2008, 371, 818
18. Chen, P. R.; Groff, D.; Guo, J.; Ou, W.; Cellitti, S.; Geierstanger, B. H.; Schultz, P. G. Angew. Chem., Int. Ed. 2009, 48, 4052
19. Gautier, A.; Nguyen, D. P.; Lusic, H.; An, W.; Deiters, A.; Chin, J. W. J. Am. Chem. Soc. 2010, 132, 4086
20. Xiao, H.; Chatterjee, A.; Choi, S. H.; Bajjuri, K. M.; Sinha, S. C.; Schultz, P. G. Angew. Chem., Int. Ed. 2013, 52, 14080
21. Elliott, T. S.; Townsley, F. M.; Bianco, A.; Ernst, R. J.; Sachdeva, A.; Elsasser, S. J.; Davis, L.; Lang, K.; Pisa, R.; Greiss, S.; Lilley, K. S.; Chin, J. W. Nat. Biotechnol. 2014, 32, 465
22. Nguyen, D. P.; Lusic, H.; Neumann, H.; Kapadnis, P. B.; Deiters, A.; Chin, J. W. J. Am. Chem. Soc. 2009, 131, 8720
23. Miyake-Stoner, S. J.; Refakis, C. A.; Hammill, J. T.; Lusic, H.; Hazen, J. L.; Deiters, A.; Mehl, R. A. Biochemistry 2010, 49, 1667
24. Chatterjee, A.; Sun, S. B.; Furman, J. L.; Xiao, H.; Schultz, P. G. Biochemistry 2013, 52, 10
25. Kolosov, P.; Frolova, L.; Seit-Nebi, A.; Dubovaya, V.; Kononenko, A.; Oparina, N.; Justesen, J.; Efimov, A.; Kisselev, L. Nucleic Acids Res. 2005, 33, 6418
26. Bulygin, K. N.; Khairulina, Y. S.; Kolosov, P. M.; Ven'yaminova, A. G.; Graifer, D. M.; Vorobjev, Y. N.; Frolova, L. Y.; Kisselev, L. L.; Karpova, G. G. RNA 2010, 16, 1902
27. Seit-Nebi, A.; Frolova, L.; Kisselev, L. EMBO Rep. 2002, 3, 881
28. Eliseev, B.; Kryuchkova, P.; Alkalaeva, E.; Frolova, L. Nucleic Acids Res. 2011, 39, 599
29. Lekomtsev, S.; Kolosov, P.; Bidou, L.; Frolova, L.; Rousset, J. P.; Kisselev, L. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 10824
30. Kryuchkova, P.; Grishin, A.; Eliseev, B.; Karyagina, A.; Frolova, L.; Alkalaeva, E. Nucleic Acids Res. 2013, 41, 4573
31. Grentzmann, G.; Ingram, J. A.; Kelly, P. J.; Gesteland, R. F.; Atkins, J. F. RNA 1998, 4, 479
32. Keeling, K. M.; Lanier, J.; Du, M.; Salas-Marco, J.; Gao, L.; Kaenjak-Angeletti, A.; Bedwell, D. M. RNA 2004, 10, 691
33. Salas-Marco, J.; Fan-Minogue, H.; Kallmeyer, A. K.; Klobutcher, L. A.; Farabaugh, P. J.; Bedwell, D. M. Mol. Cell. Biol. 2006, 26, 438
34. Conard, S. E.; Buckley, J.; Dang, M.; Bedwell, G. J.; Carter, R. L.; Khass, M.; Bedwell, D. M. RNA 2012, 18, 1210
35. Le Goff, X.; Philippe, M.; Jean-Jean, O. Mol. Cell. Biol. 1997, 17, 3164
36. Merkulova, T. I.; Frolova, L. Y.; Lazar, M.; Camonis, J.; Kisselev, L. L. FEBS Lett. 1999, 443, 41
37. Cheng, Z.; Saito, K.; Pisarev, A. V.; Wada, M.; Pisareva, V. P.; Pestova, T. V.; Gajda, M.; Round, A.; Kong, C.; Lim, M.; Nakamura, Y.; Svergun, D. I.; Ito, K.; Song, H. Genes Dev. 2009, 23, 1106
38. Kononenko, A. V.; Mitkevich, V. A.; Dubovaya, V. I.; Kolosov, P. M.; Makarov, A. A.; Kisselev, L. L. Proteins 2008, 70, 388
39. des Georges, A.; Hashem, Y.; Unbehaun, A.; Grassucci, R. A.; Taylor, D.; Hellen, C. U.; Pestova, T. V.; Frank, J. Nucleic Acids Res. 2014, 42, 3409
40. Janzen, D. M.; Geballe, A. P. Nucleic Acids Res. 2004, 32, 4491
41. Liang, H.; Wong, J. Y.; Bao, Q.; Cavalcanti, A. R.; Landweber, L. F. J. Mol. Evol. 2005, 60, 337