Detection of Pig-a mutant erythrocytes in the peripheral blood of rats and mice

Methods in Molecular Biology

Volumn 1105, Issue , 2014, Pages 205-221

  Dobrovolsky, Vasily N ^a   Cao, Xuefei ^a   Bhalli, Javed A ^a   Heflich, Robert H ^a  

^a NONE

Author keywords

Antibodies; CD24 surface marker; CD59 surface marker; Flow cytometry; Gene mutation; Glycosyl phosphatidyl inositol; Reticulocytes

Indexed keywords

PHOSPHATIDYLINOSITOL; UNCLASSIFIED DRUG; X LINKED PHOSPHATIDYL INOSITOL GLYCAN; GLYCOSYLPHOSPHATIDYLINOSITOL;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; BLOOD VOLUME; CELL LABELING; CELL MUTANT; CLINICAL ASSESSMENT; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; FREQUENCY ANALYSIS; FREQUENCY MODULATION; GENE MUTATION; HIGH THROUGHPUT SCREENING; IMMUNOFLUORESCENCE TEST; IN VIVO STUDY; MEASUREMENT ACCURACY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; REPORTER GENE; RETICULOCYTE; SAPHENOUS VEIN; TAIL VEIN; X LINKED PHOSPHATIDYL INOSITOL GLYCAN CLASS A GENE; ANIMAL; CELL SEPARATION; GENE FREQUENCY; GENETICS; METABOLISM; MUTATION;

ANIMALS; CELL SEPARATION; ERYTHROCYTES; FLOW CYTOMETRY; GENE FREQUENCY; GLYCOSYLPHOSPHATIDYLINOSITOLS; MICE; MUTATION; RATS;

EID: 84908546258     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-739-6_17     Document Type: Article

References (23)

1. Ames BN, Durston WE, Yamasaki E, Lee FD (1973) Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A 70:2281–2285
2. Vogelstein B, Kinzler KW (2004) Cancer genes and the pathways they control. Nat Med 10: 789–799
3. Cimino MC (2006) Comparative overview of current international strategies and guidelines for genetic toxicology testing for regulatory purposes. Environ Mol Mutagen 47:362–390
4. Aidoo A, Morris SM, Casciano DA (1997) Development and utilization of the rat lymphocyte hprt mutation assay. Mutat Res 387: 69–88
5. Dobrovolsky VN, Casciano DA, Heflich RH (1999) Tk +/− mouse model for detecting in vivo mutation in an endogenous, autosomal gene. Mutat Res 423:125–136
6. Jones IM, Burkhart-Schultz K, Carrano AV (1985) A method to quantify spontaneous and in vivo induced thioguanine-resistant mouse lymphocytes. Mutat Res 147:97–105
7. Stambrook PJ, Shao C, Stockelman M, Boivin G, Engle SJ, Tischfield JA (1996) APRT : a versatile in vivo resident reporter of local mutation and loss of heterozygosity. Environ Mol Mutagen 28:471–482
8. Parsons BL, Heflich RH (1997) Genotypic selection methods for the direct analysis of point mutations. Mutat Res 387:97–121
9. Dycaico MJ, Provost GS, Kretz PL, Ransom SL, Moores JC, Short JM (1994) The use of shuttle vectors for mutation analysis in transgenic mice and rats. Mutat Res 307:461–478
10. Nohmi T, Masumura KI (2004) Gpt delta transgenic mouse: a novel approach for molecular dissection of deletion mutations in vivo. Adv Biophys 38:97–121
11. Hayashi H, Kondo H, Masumura K, Shindo Y, Nohmi T (2003) Novel transgenic rat for in vivo genotoxicity assays using 6-thioguanine and Spi- selection. Environ Mol Mutagen 41: 253–259
12. Gossen JA, de Leeuw WJ, Tan CH et al (1989) Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo. Proc Natl Acad Sci U S A 86:7971–7975
13. Dobrovolsky VN, Shaddock JG, Mittelstaedt RA et al (2009) Evaluation of Macaca mulatta as a model for genotoxicity studies. Mutat Res 673:21–28
14. Miura D, Dobrovolsky VN, Kasahara Y, Katsuura Y, Heflich RH (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat. Environ Mol Mutagen 49:614–621
15. Phonethepswath S, Bryce SM, Bemis JC, Dertinger SD (2008) Erythrocyte-based Pig-a gene mutation assay: demonstration of crossspecies potential. Mutat Res 657:122–126
16. Bryce SM, Bemis JC, Dertinger SD (2008) In vivo mutation assay based on the endogenous Pig-a locus. Environ Mol Mutagen 49:256–264
17. Kinoshita T, Fujita M, Maeda Y (2008) Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress. J Biochem 144:287–294
18. Miura D, Dobrovolsky VN, Kimoto T, Kasahara Y, Heflich RH (2009) Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N -ethyl- N -nitrosourea. Mutat Res 677:86–92
19. Dertinger SD, Phonethepswath S, Franklin D et al (2010) Integration of mutation and chromosomal damage endpoints into 28-day repeat dose toxicology studies. Toxicol Sci 115:401–411
20. Dobrovolsky VN, Boctor SY, Twaddle NC et al (2010) Flow cytometric detection of Pig-A mutant red blood cells using an erythroidspecific antibody: application of the method for evaluating the in vivo genotoxicity of methylphenidate in adolescent rats. Environ Mol Mutagen 51:138–145
21. Phonethepswath S, Franklin D, Torous DK et al (2010) Pig-a mutation: kinetics in rat erythrocytes following exposure to five prototypical mutagens. Toxicol Sci 114:59–70
22. Dobrovolsky VN, Elespuru RK, Bigger CA, Robison TW, Heflich RH (2011) Monitoring humans for somatic mutations in the endogenous PIG-A gene using red blood cells. Environ Mol Mutagen 52:784–794
23. Grant SG, Jensen RH (1993) Use of hematopoietic cells and markers for the detection and quantitation of human in vivo somatic mutation. In: Garratty G (ed) Immunobiology of transfusion medicine. Marcel Dekker, New York, pp 299–323