Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat

Environmental and Molecular Mutagenesis

Volumn 49, Issue 8, 2008, Pages 614-621

  Miura, Daishiro ^a,b   Dobrovolsky, Vasily N ^a   Kasahara, Yoshinori ^b   Katsuura, Yasuhiro ^b   Heflich, Robert H ^a,c  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

^b TEIJIN PHARMA LIMITED   (Japan)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Alexa 488 labeled modified proaerolysin; F344 rats; Glycosylphosphatidylinositol; N ethyl N nitrosourea

Indexed keywords

CD48 ANTIGEN; CD59 ANTIGEN; ETHYLNITROSOUREA; GLYCOSYLPHOSPHATIDYLINOSITOL;

ANIMAL CELL; ARTICLE; CELL SURFACE; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; GENE; GENE MUTATION; MALE; NONHUMAN; PHENOTYPE; PIG A GENE; RAT; T LYMPHOCYTE;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD59; ERYTHROCYTES; ETHYLNITROSOUREA; FLOW CYTOMETRY; GLYCOSYLPHOSPHATIDYLINOSITOLS; MALE; MEMBRANE PROTEINS; MUTAGENICITY TESTS; MUTAGENS; RATS; RATS, INBRED F344; SPLEEN; T-LYMPHOCYTES;

ALEXA; MAMMALIA; RATTUS; SUIDAE;

EID: 54249128207     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.20414     Document Type: Article

References (36)

1. Aidoo A, Morris SM, Casciano DA. 1997. Development and utilization of the rat lymphocyte hprt mutation assay. Mutat Res 387:69-88.
2. Albertini RJ, Nicklas JA, O'Neill JP. 1993. Somatic cell gene mutations in humans: Biomarkers for genotoxicity. Environ Health Perspect 101(Suppl 3):193-201.
3. Araten DJ, Nafa K, Pakdeesuwan K, Luzzatto L. 1999. Clonal populations of hematopoietic cells with paroxysmal nocturnal hemoglobinuria genotype and phenotype are present in normal individuals. Proc Natl Acad Sci USA 96:5209-5214.
4. Brodsky RA, Hu R. 2006. PIG-A mutations in paroxysmal nocturnal hemoglobinuria and in normal hematopoiesis. Leuk Lymphoma 47:1215-1221.
5. Brodsky RA, Mukhina GL, Li S, Nelson KL, Chiurazzi PL, Buckley JT, Borowitz MJ. 2000. Improved detection and characterization of paroxysmal nocturnal hemoglobinuria using fluorescent aerolysin. Am J Clin Pathol 114:459-466.
6. Bryce S, Bemis J, Dertinger S. 2008. In vivo mutation assay based on the endogenous Pig-a locus. Environ Mol Mutagen 49:256-264.
7. Cimino MC. 2006. Comparative overview of current international strategies and guidelines for genetic toxicology testing for regulatory purposes. Environ Mol Mutagen 47:362-390.
8. Cross GA. 1990. Glycolipid anchoring of plasma membrane proteins. Annu Rev Cell Biol 6:1-39.
9. Dobrovolsky VN, Shaddock JG, Heflich RH. 2005. Analysis of in vivo mutation in the Hprt and Tk genes of mouse lymphocytes. In: Keohavong P, Grant SG, editors. Methods in Molecular Biology, Vol. 291: Molecular Toxicology Protocols. Totowa, NJ: Humana Press. pp 133-144.
10. Funabashi K, Okada N, Matsuo S, Yamamoto T, Morgan BP, Okada H. 1994. Tissue distribution of complement regulatory membrane proteins in rats. Immunology 81:444-451.
11. Garnett D, Williams AF. 1994. Homotypic adhesion of rat B cells, but not T cells, in response to cross-linking of CD48. Immunology 81:103-110.
12. Hughes TR, Piddlesden SJ, Williams JD, Harrison RA, Morgan BP. 1992. Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J 284:169-176.
13. ICH S2B. 1997. Genotoxicity: A standard battery for genotoxicity testing of pharmaceuticals. Available at www.ich.org.
14. Jensen RH, Bigbee WL, Langlois RG. 1987. In vivo somatic mutations in the glycophorin A locus of human erythroid cells. In: Moore MM, DeMarini DM, Tindall KR, editors. Mammalian Cell Mutagen. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. pp 149-159.
15. Kakiuchi S, Kobayashi M, Satomi Y, Miura D, Kasahara Y, Kondo S. 2006. Flow cytometric analysis of erythropoietic abnormality: Changes in the cell maturity index of reticulocytes and retic distribution index are useful as indicators of erythropoietic toxicity in non-clinical studies. J Toxicol Sci 31:111-122.
16. Kawagoe K, Takeda J, Endo Y, Kinoshita T. 1994. Molecular cloning of murine Pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus. Genomics 23:566-574.
17. Keller P, Payne JL, Tremml G, Greer PA, Gaboli M, Pandolfi PP, Bessler M. 2001. FES-Cre targets phophatidylinositol glycan class A (PIGA) inactivation to hematopoietic stem cells in the bone marrow. J Exp Med 194:581-589.
18. Kirkland D, Aardema M, Henderson L, Muller L. 2005. Evaluation of the ability of battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. I. Sensitivity, specificity and relative predictivity. Mutat Res 584:1-256.
19. Lambert IB, Singer TM, Boucher SE, Douglas GR. 2005. Detailed review of transgenic rodent mutation assays. Mutat Res 590:1-280.
20. Langlois RG, Nisbet B, Bigbee WL, Ridinger DN, Jensen RH. 1990. An improved flow cytometric assay for somatic mutations at the glycophorin A locus in humans. Cytometry 11:513-521.
21. Lecoeur H, Ledru E, Prevost M-C, Gougeon M-L. 1997. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods. J Immunol Methods 209:111-123.
22. Low MG. 1989. The glycosyl-phosphatidylinositol anchor of membrane proteins. Biochim Biophys Acta 988:427-454.
23. Miura D, Dobrovolsky VN, Mittelstaedt RA, Kasahara Y, Katsuura Y, Heflich RH. 2008. Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants. Environ Mol Mutagen 49:622-630.
24. Murakami Y, Kinoshita T, Maeda Y, Nakano T, Kosaka H, Takada J. 1999. Different roles of glycosylphosphatidylinositol in various hematopoietic cells as revealed by a mouse model of paroxysmal nocturnal hemoglobinuria. Blood 94:2963-2970.
25. Nishimura J, Murakami Y, Kinoshita T. 1999. Paroxysmal nocturnal hemoglobinuria: An acquired genetic disease. Am J Hematol 62:175-182.
26. Ringler DH, Dabich L. 1979. Hematology and clinical biochemistry. In: Baker HJ, Lindsey JR, Weisbroth SH, editors. The Laboratory Rat, Vol. 1: Biology and Diseases. San Diego, CA: Academic Press. pp 105-121.
27. Rossjohn J, Raja SM, Nelson KL, Feli SC, van der Goot FG, Parker MW, Buckley JT. 1998. Movement of a loop in domain 3 of aerolysin is required for channel formation. Biochemistry 37:741-746.
28. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV. 1992. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204-208.
29. Snyder RD, Green JW. 2001. A review of the genotoxicity of marketed pharmaceuticals. Mutat Res 488:151-169.
30. Sunderland CA, McMaster WR, Williams AF. 1979. Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes. Eur J Immunol 9:155-159.
31. Takeda J, Miyata T, Kawagoe K, Iida Y, Endo Y, Fujita T, Takahashi M, Kitani T, Kinoshita T. 1993. Deficiency of the GPI anchor caused by somatic mutation of PIG-A gene in paroxysmal nocturnal hemoglobinuria. Cell 72:703-711.
32. Valli VE, McGrath JP. 1997. Comparative leukocyte biology and toxicology. In: Bloom JC, editor. Comprehensive Toxicology, Vol. 4: Toxicology of the Hematopoietic System. Oxford, UK: Elsevier Science. pp 201-215.
33. 2 terminal domain of rat CD2 binds rat CD48 with a low affinity and binding dose not require glycosylation of CD2. Eur J Immunol 23:1373-1377.
34. Ware RE, Pickens CV, DeCastro CM, Howard TA. 2001. Circulating PIG-A mutant T lymphocytes in healthy adults and patients with bone marrow failure syndromes. Exp Hematol 29:1403-1409.
35. Watanabe R, Inoue N, Westfall B, Taron CH, Orlean P, Takeda J, Kinoshita T. 1998. The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1. EMBO J 17:877-885.
36. Wollett GR, Barclay AN, Puklavec M, Williams AF. 1985. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T, B lymphocytes. Eur J Immunol 15:163-173.