Protein engineering of the n-terminus of NEMO: Structure stabilization and rescue of ikkβ binding

Biochemistry

Volumn 53, Issue 43, 2014, Pages 6776-6785

  Guo, Bingqian ^a   Audu, Christopher O ^b   Cochran, Jared C ^c   Mierke, Dale F ^a   Pellegrini, Maria ^a  

^a Lamont Doherty Earth Observatory   (United States)

^b DARTMOUTH MEDICAL SCHOOL   (United States)

^c INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; NUCLEAR MAGNETIC RESONANCE; PROTEINS; SCAFFOLDS (BIOLOGY);

CONFORMATIONAL HETEROGENEITY; FLUORESCENCE ANISOTROPY; HIGH THERMAL STABILITY; NUCLEAR MAGNETIC RESONANCE(NMR); STRUCTURAL CHARACTERISTICS; STRUCTURAL CHARACTERIZATION; STRUCTURAL REQUIREMENTS; STRUCTURE STABILIZATION;

X RAY CRYSTALLOGRAPHY;

I KAPPA B KINASE BETA; I KAPPA B KINASE GAMMA; I KAPPA B KINASE; IKBKG PROTEIN, HUMAN; PROTEIN BINDING;

AMINO TERMINAL SEQUENCE; ANISOTROPY; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; MELTING POINT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; BINDING SITE; CHEMISTRY; GENETICS; HUMAN; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE;

BINDING SITES; CRYSTALLOGRAPHY, X-RAY; HUMANS; I-KAPPA B KINASE; MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY;

EID: 84908504883     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi500861x     Document Type: Article

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