Mutation of nonessential cysteines shows that the NF-κB essential modulator forms a constitutive noncovalent dimer that binds IκB kinase-β with high affinity

Biochemistry

Volumn 52, Issue 51, 2013, Pages 9141-9154

  Cote, Shaun M ^a   Gilmore, Thomas D ^a   Shaffer, Robert ^a   Weber, Urs ^a   Bollam, Rishitha ^a   Golden, Mary S ^a,b   Glover, Kimberley ^a   Herscovitch, Melanie ^a,c   Ennis, Thomas ^a   Allen, Karen N ^a   Whitty, Adrian ^a  

^a USA   (United States)

^b VERTEX PHARMACEUTICALS   (United States)

^c Arvid Wallgrens Backe 20   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CELL CULTURE; CELL DEATH; COVALENT BONDS; ENZYMES;

BINDING AFFINITIES; BIOCHEMICAL PROPERTIES; CATALYTIC SUBUNITS; FLUORESCENCE ANISOTROPY; INTERCHAIN DISULFIDE BONDS; SECONDARY STRUCTURES; STRUCTURAL CHARACTERIZATION; TUMOR NECROSIS FACTORS;

AMINO ACIDS;

CYSTEINE; DIMER; DODECYL SULFATE SODIUM; I KAPPA B KINASE ALPHA; I KAPPA B KINASE BETA; I KAPPA B KINASE GAMMA; CYSTINE; I KAPPA B KINASE; IKBKB PROTEIN, HUMAN; IKBKB PROTEIN, MOUSE; IKBKG PROTEIN, HUMAN; MUTANT PROTEIN; NEMO PROTEIN, MOUSE; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; ZINC FINGER PROTEIN;

AMINO ACID SEQUENCE; ANISOTROPY; ARTICLE; BINDING ASSAY; BIOCHEMICAL COMPOSITION; CIRCULAR DICHROISM; DNA SEQUENCE; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; MAMMAL CELL; MOLECULAR WEIGHT; MUTATION; OLIGOMERIZATION; OXIDATION REDUCTION STATE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WESTERN BLOTTING; ANIMAL; CELL CULTURE; CHEMISTRY; DIMERIZATION; GENETICS; HUMAN; KINETICS; KNOCKOUT MOUSE; METABOLISM; MOUSE; OXIDATION REDUCTION REACTION; PROTEIN DOMAIN; PROTEIN QUATERNARY STRUCTURE; PROTEIN STABILITY; SOLUBILITY;

ANIMALS; CELLS, CULTURED; CYSTEINE; CYSTINE; DIMERIZATION; HUMANS; I-KAPPA B KINASE; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KINETICS; MICE; MICE, KNOCKOUT; MUTANT PROTEINS; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STABILITY; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS; SOLUBILITY; ZINC FINGERS;

EID: 84895776854     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi401368r     Document Type: Article

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