Recombinant production of TEV cleaved human parathyroid hormone

Journal of Peptide Science

Volumn 19, Issue 8, 2013, Pages 504-510

  Audu, Christopher O ^a   Cochran, Jared C ^b   Pellegrini, Maria ^a   Mierke, Dale F ^a  

^a Lamont Doherty Earth Observatory   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Circular dichroism; LC MS; NMR; Parathyroid hormone; Recombinant peptide production; TEV protease

Indexed keywords

NITROGEN 15; PARATHYROID HORMONE; PLASMID VECTOR; PROTEINASE; THIOREDOXIN;

ARTICLE; CIRCULAR DICHROISM; ENZYME PURIFICATION; GEL PERMEATION CHROMATOGRAPHY; HETERONUCLEAR NUCLEAR MAGNETIC RESONANCE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; TOBACCO VIRUS; VIRUS;

CIRCULAR DICHROISM; LC/MS; NMR; PARATHYROID HORMONE; RECOMBINANT PEPTIDE PRODUCTION; TEV PROTEASE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ENDOPEPTIDASES; ESCHERICHIA COLI; HUMANS; MOLECULAR SEQUENCE DATA; PARATHYROID HORMONE; PROTEIN STRUCTURE, SECONDARY; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS;

TOBACCO ETCH VIRUS;

EID: 84880333120     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.2528     Document Type: Article

References (29)

1. Ramasamy L. Recent advances in physiological calcium homeostasis. Clin. Chem. Lab. Med. 2006; 44: 237-273.
2. Aslan D, Andersen MD, Gede LB, de Franca T, Jorgensen SR, Schwarz P, Jorgensen NR, Mechanisms for the bone anabolic effect of parathyroid hormone treatment in humans. Scand. J. Clin. Lab. Invest. 2012; 72: 14-22.
3. Habener JF, Rosenblatt M, Potts JT Jr. Parathyroid hormone: biochemical aspects of biosynthesis, secretion, action and metabolism. Physiol. Rev. 1984; 64: 985-1053.
4. Habener JF, Potts JT Jr. Subcellular distributions of parathyroid hormone, hormonal precursors, and parathyroid secretory protein. Endocrinology 1979; 104: 265-275.
5. Gardella TJ, Jüppner H. Interaction of PTH and PTHrP with their receptors. Rev. Endocr. Metab. Disord. 2000; 1: 317-329
6. Rabbani SA, Yasuda T, Bennett HP, Sung WL, Zahab DM, Tam CS, Goltzman D, Hendy GN. Recombinant human parathyroid hormone synthesized in Escherichia coli. Purification and characterization. J. Biol. Chem. 1988; 263: 1307-1313.
7. Harder MP, Sanders EA, Wingender E, Deckwer WD. Studies on the production of human parathyroid hormone by recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 1993; 39: 329-334.
8. Wingender E, Bercz G, Blocker H, Frank R, Mayer H. Expression of human parathyroid hormone in Escherichia coli. J. Biol. Chem. 1989; 264: 4367-4373.
9. Hogset A, Blingsmo OR, Gautvik VT, Saether O, Jacobsen PB, Gordeladze JO, Alestrom P, Gautvik KM. Expression of human parathyroid hormone in Escherichia coli. Biochem. Biophys. Res. Commun. 1990; 166: 50-60.
10. Gram H, Ramage P, Memmert K, Gamse R, Kocher HP. A novel approach for high level production of a recombinant human parathyroid hormone fragment in Escherichia coli. Biotechnology 1994; 10: 1017-1023.
11. Oldenburg KR, D'Orfani AL, Selick HE. A method for the high-level expression of a parathyroid hormone analog in Escherichia coli. Prot. Express. Purif. 1994; 5: 278-284.
12. Rokkones E, Kareem BN, Olstad OK, Hogset A, Schenstrom K, Hansson L, Gautvik KM. Expression of human parathyroid hormone in mammalian cells, Escherichia coli and Saccharomyces cerevisiae. J. Biotechnol. 1994; 33: 293-306.
13. Suzuki Y, Yabuta M, Ohsuye K. High-level production of recombinant human parathyroid hormone 1-34. Appl. Environ. Microbiol. 1998; 64: 526-529.
14. Gardella TJ, Rubin D, Abou-Samra AB, Keutmann HT, Potts JT Jr, Kronenberg HM, Nussbaum SR. Expression of human parathyroid hormone-(1-84) in Escherichia coli as a factor X-cleavable fusion protein. J. Biol. Chem. 1990; 265: 15854-15859.
15. Terpe K. Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 2006; 72: 211-222.
16. Liu Q, Lin J, Liu M, Tao X, Wei D, Ma X, Yang S. Large scale preparation of recombinant human parathyroid hormone 1-84 from Escherichia coli. Prot. Express. and Purif. 2007; 54: 212-219.
17. Rolz C, Pellegrini M, Mierke DF. Molecular characterization of the receptor-ligand complex for parathyroid hormone. Biochemistry 1999; 38: 6397-6405.
18. Whitmore L, Wallace B. DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Res., 2004; 32: W668-W673.
19. Morrow JA, Segall ML, Lund-katz S, Phillips MC, Knapp M, Rupp B, Weisgraber K. Differences in stability among the human apolipoprotein e isoforms determined by the amino-terminal domain. Biochemistry 2000; 39: 11657-11666.
20. Savitzky A, Golay MJE. Smoothing and differentiation of data by a simplified least squares procedure. Anal. Chem. 1964; 36: 1627-1639.
21. McCoy J, LaVallie E. Expression and purification of thioredoxin fusion proteins. Curr. Prot. Mol. Bio. 2001; 28: 16.8.1-16.8.14.
22. Pellegrini M, Royo M, Rosenblatt M, Chorev M, Mierke DF. Addressing the tertiary structure of human parathyroid hormone-(1-34)*. J. Biol. Chem. 1998; 273: 10420-10427.
23. Barbier J-R, Gardella TJ, Dean T, Maclean S, Potetinova Z, Whitfield JF, Willick GE. Backbone-methylated analogues of the principle receptor binding region of human parathyroid hormone. J. Biol. Chem. 2005; 280: 23771-23777.
24. Zull J, Smith SK, Wiltshire R. Effect of methionine oxidation and deletion of amino-terminal residues on the conformation of parathyroid hormone: circular dichroism studies. J. Biol. Chem. 1990; 285: 5671-5676.
25. Scian M, Marin M, Bellanda M, Tou L, Alexander J, Rosenblatt M, Chorev M, Peggion E, Mammi S. Backbone dynamics of human parathyroid hormone (1-34): flexibility of the central region under different environmental conditions. Biopolymers 2006; 84: 147-160.
26. Li Y. Recombinant production of antimicrobial peptides in Escherichia coli: a review. Protein Express. Purif. 2011; 80: 260-267.
27. Bellot G, Pascal R, Mendre C, Urbach S, Mouillac B, Demene H. Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor. Prot. Express. Purif. 2011; 78: 131-138.
28. Kapust RB, Tozser J, Copeland TD, Waugh DS. The P1' specificity of tobacco etch virus protease. Biochem. Biophys. Res. Comm. 2002; 294: 949-955.
29. Cai M, Huang Y, Kazuasu S, Clore GM, Gronenborn A, Craigie, R. An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli. J. Biomol. NMR 1998; 11: 97-102.