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Volumn 322, Issue , 2000, Pages 325-345

Production of recombinant TRAIL and TRAIL receptor: Fc chimeric proteins

(1)  Schneider, Pascal a  

a NONE

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVIEW;

EID: 0033911632     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0076-6879(00)22031-4     Document Type: Review
Times cited : (96)

References (25)
  • 22
    • 0031578808 scopus 로고    scopus 로고
    • K. B. Tan, J. Harrop, M. Reddy, P. Young, J. Terrett, J. Emery, G. Moore, and A. Truneh, Gene 204, 35 (1997). ** A few nucleotides at the 5′ end of the primer that do not match the cDNA sequence are sometimes removed during the PCR. The addition of "protecting" nucleotides ensures that the restriction site will not be destroyed.
    • (1997) Gene , vol.204 , pp. 35
    • Tan, K.B.1    Harrop, J.2    Reddy, M.3    Young, P.4    Terrett, J.5    Emery, J.6    Moore, G.7    Truneh, A.8
  • 24
    • 0019887744 scopus 로고
    • C. Bordier, J. Biol. Chem. 256, 1604 (1981). * Precondensed Triton X-114 is obtained by subjecting a 2% (v/v) solution of commercial-grade Triton X-114 in PBS to three cycles of phase separation. One cycle consists of two steps, one at 4° and the other at 37°, the latter yielding a detergent-rich lower phase consisting of 12% (v/v) Triton X-114 and a detergent-depleted upper phase, which is discarded. The detergent-rich phase is diluted to 2% (v/v) with fresh PBS before the next cycle. For large volumes, no centrifugation is required as phase separation will occur within 16 hr at 37°. After the third cycle, the detergent phase can be stored for years at -20°. ** M2-agarose can be conveniently packed in a used HiTrap column (Pharmacia). To this purpose, remove the red top and cut 2-mm plastic at the top of the column, using a razor blade. Remove the black plug and empty the column, taking care to save the filters. Empty HiTrap columns are not available. Alternatively, use any kind of column. * PMS is light sensitive. Always keep protected from light.
    • (1981) J. Biol. Chem. , vol.256 , pp. 1604
    • Bordier, C.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.