Adaptation for survival: Phenotype and transcriptome response of CHO cells to elevated stress induced by agitation and sparging

Journal of Biotechnology

Volumn 189, Issue , 2014, Pages 94-103

  Sieck, Jochen B ^a,b   Budach, Wolfgang E ^a   Suemeghy, Zoltan ^a   Leist, Christian ^a   Villiger, Thomas K ^a   Morbidelli, Massimo ^b   Soos, Miroslav ^b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

CHO cells; Environomics; MRNA microarrays; Stirred bioreactors; Stress adaptation; Stress response; Transcriptome analysis; Transcriptome fingerprinting

Indexed keywords

CHO CELL; ENVIRONOMICS; STIRRED BIOREACTORS; STRESS RESPONSE; TRANSCRIPTOME ANALYSIS; TRANSCRIPTOMES;

GLUCOSE; GLUTAMINE; MESSENGER RNA; TRANSCRIPTOME; RNA, MESSENGER;

ADAPTATION; AMMONIA FORMATION; ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CELL STRESS; CELL SURVIVAL; CELL VIABILITY; CHO CELL LINE; CONTROLLED STUDY; FED BATCH CULTURE; FEMALE; GLYCOSYLATION; GROWTH RATE; HYDRODYNAMICS; LIMIT OF QUANTITATION; METABOLIC RATE; NONHUMAN; PHENOTYPE; SHEAR STRESS; SPARGING; STIRRED REACTOR; ANIMALS; BIOREACTORS; CHO CELLS; CRICETULUS; GENETICS; MICROBIOLOGY;

ANIMALS; BIOREACTORS; CHO CELLS; CRICETULUS; RNA, MESSENGER; TRANSCRIPTOME;

EID: 84908323452     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.08.042     Document Type: Article

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