Effects of bubble-liquid two-phase turbulent hydrodynamics on cell damage in sparged bioreactor

Biotechnology Progress

Volumn 30, Issue 1, 2014, Pages 48-58

  Liu, Yang ^a   Li, Fanxing ^a   Hu, Weiwei ^b   Wiltberger, Kelly ^b   Ryll, Thomas ^b  

^a North Carolina State University   (United States)

^b BIOGEN   (United States)

Author keywords

Bubble liquid two phase turbulent flows; Cell death; Hydrodynamics; Sparged bioreactor

Indexed keywords

BUBBLE COLUMN REACTORS; FLUCTUATION VELOCITIES; HYDRODYNAMIC STUDIES; SECOND ORDER MOMENT; TURBULENT ENERGY DISSIPATION RATE; TURBULENT ENERGY PRODUCTION; TURBULENT ENERGY TRANSFER; TURBULENT KINETIC ENERGY;

BIOCONVERSION; BIOREACTORS; CELL DEATH; ENERGY TRANSFER; GASES; HYDRODYNAMICS; KINETICS; TURBULENT FLOW;

LIQUIDS;

ARTICLE; BIOREACTOR; COMPUTER SIMULATION; CULTURE TECHNIQUE; EQUIPMENT; GAS; HYDRODYNAMICS; KINETICS; MECHANICAL STRESS; METHODOLOGY; REPRODUCIBILITY; THEORETICAL MODEL;

BIOREACTORS; CELL CULTURE TECHNIQUES; COMPUTER SIMULATION; GASES; HYDRODYNAMICS; KINETICS; MODELS, THEORETICAL; REPRODUCIBILITY OF RESULTS; STRESS, MECHANICAL;

EID: 84893793023     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1790     Document Type: Article

References (44)

1. Tramper J, Williams JB, Joustra D, Vlak JM. Shear sensitivity of insect cells in suspension. Enzyme Microbiol Technol. 1986;8:33-36.
2. Tramper J, Smit D, Straatman J, Vlak JM. Bubble column design for growth of fragile insect cells. Bioprocess Eng. 1988;3:37-41.
3. Jöbses I, Martens D, Tramper J. Lethal events during gas sparging in animal cell culture. Biotechnol Bioeng. 1991;37:484-490.
4. Cherry RS, Hulle CT. Cell death in the thin films of bursting bubbles. Biotechnol Prog. 1992;8:11-18.
5. Handa A, Emery AN, Spier RE. On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors. Dev Biol Stand. 1987;66:241-253.
6. Michaels JD, Mallik AK, Papoutsakis ET. Sparging and agitation induced injury of cultured animals cells: do cell-to-bubble interactions in the bulk liquid injure cells? Biotechnol Bioeng. 1996;51:399-409.
7. Papoutsakis ET. Fluid-mechanical damage of animal cells in bioreactors. Trends Biotechnol. 1991;9:427-437.
8. Trinh K, Garcia-Briones M, Chalmers JJ, Hink F. Quantification of damage to suspended insect cells as a result of bubble rupture. Biotechnol Bioeng. 1994;43:37-45.
9. 2 fixation by Dunaliella tertiolecta. J Chem Technol Biotechnol. 1995;62:351-358.
10. Camacho FG, Gomez AC, Sobczuk TM, Grima EM. Effects of mechanical and hydrodynamic stress in agitated, sparged cultures of Porphyridium cruentum. Proc Biochem. 2000;35:1045-1050.
11. Meier SJ, Hatton TA, Wang DI. Cell death from bursting bubbles: role of cell attachment to rising bubbles in sparged reactors. Biotechnol Bioeng. 1999;62:468-478.
12. Zhu Y, Cuenca V Y, Zhou WC, Varma A. NS0 cell damage by high gas velocity sparging in protein-free and cholesterol-free cultures. Biotechnol Bioeng. 2008;101:751-760.
13. Barbosa MJ, Wijffels RH. Overcoming shear stress of microalgae culture in sparged photobioreactors. Biotechnol Bioeng. 2004;85:78-85.
14. Barbosa MJ, Albrecht M, Wijffels RH. Hydrodynamic stress and lethal events in sparged microalgae cultures. Biotechnol Bioeng. 2003;83:112-120.
15. Gudin C, Chaumont D. Cell fragility-the key problem of microalgae mass production in closed photobioreactors. Bioresour Technol. 1991:38:145-151.
16. Miguel A, Garcia B, Chalmers JJ. Flow parameters associated with hydrodynamic cell injury. Biotechnol Bioeng. 1994;4:1089-1098.
17. Chalmers JJ. Shear sensitivity of insect cells. Cytotechnology. 1996;20:163-171.
18. Camacho FG, Grima EM, Miron AS, Pascual VG, Chisti MY. Carboxymethyl cellulose protects algal cells against hydrodynamic stress. Enzyme Microb Technol. 2001;29:602-610.
19. Queen A, Bailey JE. Influence of serum level, cell line, flow type and viscosity on flow-induced analysis of suspended mammalian cells. Biotechnol Lett. 1989;11:531-536.
20. Cherry RS, Kwon KY. Transient shear stresses on a suspension cell in turbulence. Biotechnol Bioeng. 1990;36:563-571.
21. Bronnemeier R, Markl H. Hydrodynamic stress capacity of microorganisms. Biotechnol Bioeng. 1982;24:553-578.
22. Silva HJ, Cortinas T, Ertola HJ. Effect of hydrodynamic stress on Dunaliella growth. J Chem Technol Biotechnol. 1987;40:41-49.
23. Bluestein M, Mockros LF. Hemolytic effects of energy dissipation in flow blood. Med Biol Eng Comput. 1969;7:1-16.
24. Cherry RS, Papoutsakis ET. Hydrodynamic effects on cells in agitated tissue culture reactors. Bioprocess Eng. 1986;1:81-89.
25. Croughan MS, Hamel JF, Wang D. Hydrodynamic effect on animal cells grown in microcarrier cultures. Biotechnol Bioeng. 1987;29:130-141.
26. Ma NN, Koelling KW, Chalmers JJ. Fabrication and use of a transient contractional flow device to quantify the sensitivity of mammalian and insect cells to hydrodynamic forces. Biotechnol Bioeng. 2002;80:428-436.
27. Mollet M, Ma NN, Zhao Y, Brodkey B, Taticek R, Chalmers JJ. Bioprocess equipment: characterization of energy dissipation rate and its potential to damage cells. Biotechnol Proc. 2004;20:1437-1448.
28. Mollet M, Silva RG, Berdugo C, Chalmers JJ. Acute hydrodynamic forces and apoptosis: a complex question. Biotechnol Bioeng. 2007;98:772-788.
29. Zhou G, Kresta SM. Distribution of energy dissipation between convective and turbulent flow for three frequently used impellers. Trans Inst Chem Eng. 1996;74:379-389.
30. Zhou G, Kresta SM. Impact of tank geometry on the maximum turbulence energy dissipation rate for impellers. AIChE J. 1996;42:2476-2490.
31. Kresta S. Turbulence in stirred tanks: anisoptropic, approximate, and applied. Can J Chem Eng. 1998;76:563-575.
32. Bird RB, Stewart WE, Lightfoot EN. Transport Phenomena, 2nd ed. New York: Wiley; 2001.
33. Hu WW, Berdugo C, Chalmers JJ. The potential of hydrodynamic damage to animal cells of industrial relevance: current understanding. Cytotechnology. 2010;63:445-460.
34. Zhou LX, Yang M, Lian CY, Fan LS, Lee DJ. On the second-order moment turbulence model for simulating a bubble column. Chem Eng Sci. 2002;57:3269-3281.
35. Zhou LX, Yang M, Fan LS. A second-order moment three-phase turbulence model for simulating gas-liquid-solid flows. Chem Eng Sci. 2005;60:647-653.
36. Zhou LX, Liao CM, Chen T. A unified second-order moment two-phase turbulence model for simulating gas-particle flows. ASME-FED. 1994;185:307-331.
37. Zhou LX, Chen T. Simulation of strongly swirling gas-particle flows using USM and k-ε-kp two-phase turbulence models. Powder Technol. 2001;114:1-11.
38. Liu Y, Zhou LX, Xu CX. Large-eddy simulation of swirling gas-particle flows using a USM two-phase SGS stress model. Powder Technol. 2009;198:183-188.
39. Pantanker SV. Numerical Heat Transfer and Fluid Flow. Washington D.C.: Hemisphere Publishing Corporation; 1980.
40. Lin TJ, Reese J, Hong T, Fan LS. Quantitative analysis and computation of two-dimensional bubble columns. AIChE J. 1996;42:301-318.
41. Zhou LX. Theory and Numerical Modeling of Turbulent Gas-Particle Flows and Combustion. Beijing, China: Science Press; 1993.
42. Pope SB. Turbulent Flows. Cambridge, United Kingdom: Cambridge University Press; 2000.
43. Frisch U. Turbulence: The Legacy of A. N. Kolmogorov. Cambridge, United Kingdom: Cambridge University Press; 1995.
44. Yang GQ, Du B, Fan LS. Bubble formation and dynamics in gas-liquid-solid fluidization-a review. Chem Eng Sci. 2007;62:2-27.