Development of a Scale-Down Model of hydrodynamic stress to study the performance of an industrial CHO cell line under simulated production scale bioreactor conditions

Journal of Biotechnology

Volumn 164, Issue 1, 2013, Pages 41-49

  Sieck, Jochen B ^a,b   Cordes, Thekla ^a,c   Budach, Wolfgang E ^a   Rhiel, Martin H ^a,d   Suemeghy, Zoltan ^a   Leist, Christian ^a   Villiger, Thomas K ^a   Morbidelli, Massimo ^b   Soos, Miroslav ^b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b ETH ZURICH   (Switzerland)

^c UNIVERSITY OF LUXEMBOURG   (Luxembourg)

^d ESBATech   (Switzerland)

Author keywords

Fed batch cultivation; Local energy dissipation rate; MRNA microarrays; Scale Down Model; Transcriptome analysis

Indexed keywords

AVERAGE ENERGY DISSIPATION; CULTIVATION CONDITIONS; FED-BATCH CULTIVATION; LARGE SCALE PRODUCTIONS; LOCAL ENERGY; SCALE-DOWN; STIRRED TANK BIOREACTORS; TRANSCRIPTOME ANALYSIS;

BIOCONVERSION; CELL CULTURE; COMPUTER SIMULATION; CULTIVATION; HYDRODYNAMICS; MONOCLONAL ANTIBODIES; NUCLEIC ACIDS; PRODUCTIVITY; REPAIR;

BIOREACTORS;

AMMONIA; GLUCOSE; GLUTAMINE; MONOCLONAL ANTIBODY;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOREACTOR; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CHO CELL; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DNA REPLICATION; HYDRODYNAMIC STRESS; HYDRODYNAMICS; LARGE SCALE PRODUCTION; MICROARRAY ANALYSIS; MODEL; NONHUMAN; OSCILLATION; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN EXPRESSION; RNA ANALYSIS; SCALE DOWN MODEL; SHEAR STRESS; SIMULATION;

ANIMALS; BIOENGINEERING; BIOREACTORS; BIOTECHNOLOGY; CHO CELLS; COMPUTATIONAL BIOLOGY; CRICETINAE; GENE EXPRESSION PROFILING; HYDRODYNAMICS; MODELS, THEORETICAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA, MESSENGER; STRESS, PHYSIOLOGICAL; TRANSCRIPTOME;

EID: 84875085627     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.11.012     Document Type: Article

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