CD4 aptamer-RORγt shRNA chimera inhibits IL-17 synthesis by human CD4+ T cells

Biochemical and Biophysical Research Communications

Volumn 452, Issue 4, 2014, Pages 1040-1045

  Song, Pingfang ^a   Chou, Yuan K ^a   Zhang, Xiaowei ^a   Meza Romero, Roberto ^a   Yomogida, Kentaro ^a,b   Benedek, Gil ^a   Chu, Cong Qiu ^a  

^a PORTLAND VA MEDICAL CENTER   (United States)

^b WILLIAM BEAUMONT HOSPITAL   (United States)

Author keywords

Aptamer shRNA; ROR t; Th17

Indexed keywords

CD4 APTAMER ROR GAMMA T SHORT HAIRPIN RNA; INTERLEUKIN 17; RNA INDUCED SILENCING COMPLEX; RNA POLYMERASE; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; CD4 ANTIGEN; RETINOID RELATED ORPHAN RECEPTOR GAMMA; RORC PROTEIN, HUMAN; SMALL INTERFERING RNA;

ARTICLE; CD4+ T LYMPHOCYTE; CELL DIFFERENTIATION; CHIMERA; CONFOCAL MICROSCOPE; CONTROLLED STUDY; CYTOKINE PRODUCTION; DOWN REGULATION; DRUG DELIVERY SYSTEM; FLOW CYTOMETRY; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERNALIZATION; NUCLEOTIDE SEQUENCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; TH17 CELL; BIOSYNTHESIS; CELL LINE; GENETIC TRANSFECTION; GENETICS; IMMUNOLOGY; PROCEDURES;

ANTIGENS, CD4; CD4-POSITIVE T-LYMPHOCYTES; CELL LINE; HUMANS; INTERLEUKIN-17; NUCLEAR RECEPTOR SUBFAMILY 1, GROUP F, MEMBER 3; RNA, SMALL INTERFERING; TH17 CELLS; TRANSFECTION;

EID: 84907887593     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.09.037     Document Type: Article

References (34)

1. M. Freeley, and A. Long Advances in siRNA delivery to T-cells: potential clinical applications for inflammatory disease, cancer and infection Biochem. J. 455 2013 133 147
2. J. Gehl Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research Acta Physiol. Scand. 177 2003 437 447
3. W. Lai, C.H. Chang, and D.L. Farber Gene transfection and expression in resting and activated murine CD4 T cell subsets J. Immunol. Methods 282 2003 93 102
4. A.G. Gomez-Valades, M. Llamas, S. Blanch, J.C. Perales, J. Roman, L. Gomez-Casajus, and C. Mascaro Specific Jak3 downregulation in lymphocytes impairs gammac cytokine signal transduction and alleviates antigen-driven inflammation in vivo Mol Ther. Nucleic Acids 1 2012 e42
5. M. Rangachari, C. Zhu, K. Sakuishi, S. Xiao, J. Karman, A. Chen, M. Angin, A. Wakeham, E.A. Greenfield, R.A. Sobel, H. Okada, P.J. McKinnon, T.W. Mak, M.M. Addo, A.C. Anderson, and V.K. Kuchroo Bat3 promotes T cell responses and autoimmunity by repressing Tim-3-mediated cell death and exhaustion Nat. Med. 18 2012 1394 1400
6. H.S. Jin, L. Liao, Y. Park, and Y.C. Liu Neddylation pathway regulates T-cell function by targeting an adaptor protein Shc and a protein kinase Erk signaling Proc. Natl. Acad. Sci. U.S.A. 110 2013 624 629
7. Z. Liu, M. Winters, M. Holodniy, and H. Dai SiRNA delivery into human T cells and primary cells with carbon-nanotube transporters Angew. Chem. Int. Ed. 46 2007 2023 2027
8. A. Eguchi, B.R. Meade, Y.C. Chang, C.T. Fredrickson, K. Willert, N. Puri, and S.F. Dowdy Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein Nat. Biotechnol. 27 2009 567 571
9. J. Zhou, C.P. Neff, P. Swiderski, H. Li, D.D. Smith, T. Aboellail, L. Remling-Mulder, R. Akkina, and J.J. Rossi Functional in vivo delivery of multiplexed anti-HIV-1 siRNAs via a chemically synthesized aptamer with a sticky bridge Mol. Ther. 21 2013 192 200
10. X. Ni, M. Castanares, A. Mukherjee, and S.E. Lupold Nucleic acid aptamers: clinical applications and promising new horizons Curr. Med. Chem. 18 2011 4206 4214
11. J. Zhou, M.L. Bobbin, J.C. Burnett, and J.J. Rossi Current progress of RNA aptamer-based therapeutics Front. Genet. 3 2012 234
12. J.O. McNamara 2nd, E.R. Andrechek, Y. Wang, K.D. Viles, R.E. Rempel, E. Gilboa, B.A. Sullenger, and P.H. Giangrande Cell type-specific delivery of siRNAs with aptamer-siRNA chimeras Nat. Biotechnol. 24 2006 1005 1015
13. J. Zhou, H. Li, S. Li, J. Zaia, and J.J. Rossi Novel dual inhibitory function aptamer-siRNA delivery system for HIV-1 therapy Mol. Ther. 16 2008 1481 1489
14. L.A. Wheeler, R. Trifonova, V. Vrbanac, E. Basar, S. McKernan, Z. Xu, E. Seung, M. Deruaz, T. Dudek, J.I. Einarsson, L. Yang, T.M. Allen, A.D. Luster, A.M. Tager, D.M. Dykxhoorn, and J. Lieberman Inhibition of HIV transmission in human cervicovaginal explants and humanized mice using CD4 aptamer-siRNA chimeras J. Clin. Invest. 121 2011 2401 2412
15. L.A. Wheeler, V. Vrbanac, R. Trifonova, M.A. Brehm, A. Gilboa-Geffen, S. Tanno, D.L. Greiner, A.D. Luster, A.M. Tager, and J. Lieberman Durable knockdown and protection from HIV transmission in humanized mice treated with gel-formulated CD4 aptamer-siRNA chimeras Mol. Ther. 21 2013 1378 1389
16. P. Miossec, and J.K. Kolls Targeting IL-17 and TH17 cells in chronic inflammation Nat. Rev. Drug Discovery 11 2012 763 776
17. J.P. van Hamburg, O.B. Corneth, S.M. Paulissen, N. Davelaar, P.S. Asmawidjaja, A.M. Mus, and E. Lubberts IL-17/Th17 mediated synovial inflammation is IL-22 independent Ann. Rheum. Dis. 72 2013 1700 1707
18. J.P. van Hamburg, P.S. Asmawidjaja, N. Davelaar, A.M. Mus, E.M. Colin, J.M. Hazes, R.J. Dolhain, and E. Lubberts Th17 cells, but not Th1 cells, from patients with early rheumatoid arthritis are potent inducers of matrix metalloproteinases and proinflammatory cytokines upon synovial fibroblast interaction, including autocrine interleukin-17A production Arthritis Rheum. 63 2011 73 83
19. R. Cascao, R.A. Moura, I. Perpetuo, H. Canhao, E. Vieira-Sousa, A.F. Mourao, A.M. Rodrigues, J. Polido-Pereira, M.V. Queiroz, H.S. Rosario, M.M. Souto-Carneiro, L. Graca, and J.E. Fonseca Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis Arthritis Res. Ther. 12 2010 R196
20. C.Q. Chu, Z. Song, L. Mayton, B. Wu, and P.H. Wooley IFNgamma deficient C57BL/6 (H-2b) mice develop collagen induced arthritis with predominant usage of T cell receptor Vbeta6 and Vbeta8 in arthritic joints Ann. Rheum. Dis. 62 2003 983 990
21. E. Lubberts IL-17/Th17 targeting: on the road to prevent chronic destructive arthritis? Cytokine 41 2008 84 91
22. M.C. Genovese, F. Van den Bosch, S.A. Roberson, S. Bojin, I.M. Biagini, P. Ryan, and J. Sloan-Lancaster LY2439821, a humanized anti-interleukin-17 monoclonal antibody, in the treatment of patients with rheumatoid arthritis: a phase I randomized, double-blind, placebo-controlled, proof-of-concept study Arthritis Rheum. 62 2010 929 939
23. T. Korn, E. Bettelli, M. Oukka, and V.K. Kuchroo IL-17 and Th17 Cells Annu. Rev. Immunol. 27 2009 485 517
24. J. Zhou, and J.J. Rossi Aptamer-targeted RNAi for HIV-1 therapy Methods Mol. Biol. 721 2011 355 371
25. X. Ni, Y. Zhang, J. Ribas, W.H. Chowdhury, M. Castanares, Z. Zhang, M. Laiho, T.L. DeWeese, and S.E. Lupold Prostate-targeted radiosensitization via aptamer-shRNA chimeras in human tumor xenografts J. Clin. Invest. 121 2011 2383 2390
26. K.A. Davis, Y. Lin, B. Abrams, and S.D. Jayasena Staining of cell surface human CD4 with 2′-F-pyrimidine-containing RNA aptamers for flow cytometry Nucleic Acids Res. 26 1998 3915 3924
27. P. Zhang, N. Zhao, Z. Zeng, Y. Feng, C.H. Tung, C.C. Chang, and Y. Zu Using an RNA aptamer probe for flow cytometry detection of CD30-expressing lymphoma cells Lab. Invest. 89 2009 1423 1432
28. K. Yomogida, Y. Chou, J. Pang, B. Baravati, B.J. Maniaci, S. Wu, Y. Zhu, and C.Q. Chu Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression Cytokine 58 2012 431 436
29. K. Yomogida, Y.K. Chou, and C.Q. Chu Superantigens induce IL-17 production from polarized Th1 clones Cytokine 63 2013 6 9
30. P.R. Bouchard, R.M. Hutabarat, and K.M. Thompson Discovery and development of therapeutic aptamers Annu. Rev. Pharmacol. Toxicol. 50 2010 237 257
31. J.P. Dassie, and P.H. Giangrande Current progress on aptamer-targeted oligonucleotide therapeutics Ther. Delivery 4 2013 1527 1546
32. S. Burgler, P.Y. Mantel, C. Bassin, N. Ouaked, C.A. Akdis, and C.B. Schmidt-Weber RORC2 is involved in T cell polarization through interaction with the FOXP3 promoter J. Immunol. 184 2010 6161 6169
33. J.R. Huh, M.W. Leung, P. Huang, D.A. Ryan, M.R. Krout, R.R. Malapaka, J. Chow, N. Manel, M. Ciofani, S.V. Kim, A. Cuesta, F.R. Santori, J.J. Lafaille, H.E. Xu, D.Y. Gin, F. Rastinejad, and D.R. Littman Digoxin and its derivatives suppress TH17 cell differentiation by antagonizing RORgammat activity Nature 472 2011 486 490
34. J.R. Huh, and D.R. Littman Small molecule inhibitors of RORgammat: targeting Th17 cells and other applications Eur. J. Immunol. 42 2012 2232 2237