메뉴 건너뛰기




Volumn , Issue 83, 2014, Pages

Isolation of cortical microglia with preserved immunophenotype and functionality from murine neonates

Author keywords

Cytokines; DAMPs; Immunology; Issue 83; LPS; Neurodegeneration; Neuroinflammation; Pam3CSK4; PAMPs; TLRs

Indexed keywords

ANIMAL; ARTICLE; BRAIN CORTEX; C57BL MOUSE; CYTOLOGY; IMMUNOPHENOTYPING; METHODOLOGY; MICROGLIA; MOUSE; NEWBORN;

EID: 84907652246     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51005     Document Type: Article
Times cited : (18)

References (40)
  • 1
    • 67650966680 scopus 로고    scopus 로고
    • Microglia Physiology: Unique Stimuli, Specialized Responses
    • Ranshoff, R.M. P. V. H. Microglia Physiology: Unique Stimuli, Specialized Responses. Annu. Rev. Immunol. 27, 119-145 (2009).
    • (2009) Annu. Rev. Immunol. , vol.27 , pp. 119-145
    • Ranshoff, R.M.P.V.H.1
  • 2
    • 0025220976 scopus 로고
    • Heterogeneity in the distribution and morphology of microglia in the normal adult mouse brain
    • Lawson, L.J., Perry, V. H., Dri, P. & Gordon, S. Heterogeneity in the distribution and morphology of microglia in the normal adult mouse brain. Neuroscience. 39, 151-170 (1990).
    • (1990) Neuroscience. , vol.39 , pp. 151-170
    • Lawson, L.J.1    Perry, V.H.2    Dri, P.3    Gordon, S.4
  • 3
    • 84869156976 scopus 로고    scopus 로고
    • Development and homeostasis of "resident" myeloid cells: The case of the microglia
    • doi:10.1002/glia.22393
    • Gomez Perdiguero, E., Schulz, C. & Geissmann, F. Development and homeostasis of "resident" myeloid cells: The case of the microglia. Glia. 61, 112-120, doi:10.1002/glia.22393 (2013).
    • (2013) Glia. , vol.61 , pp. 112-120
    • Gomez Perdiguero, E.1    Schulz, C.2    Geissmann, F.3
  • 4
    • 84875892857 scopus 로고    scopus 로고
    • Microglia emerge from erythromyeloid precursors via Pu.1-and Irf8-dependent pathways
    • doi:10.1038/nn.3318
    • Kierdorf, K. et al. Microglia emerge from erythromyeloid precursors via Pu.1-and Irf8-dependent pathways. Nat. Neurosci. 16, 273-280, doi:10.1038/nn.3318 (2013).
    • (2013) Nat. Neurosci. , vol.16 , pp. 273-280
    • Kierdorf, K.1
  • 5
    • 80355143546 scopus 로고    scopus 로고
    • Microglial cell origin and phenotypes in health and disease
    • doi:10.1038/nri3086
    • Saijo, K. & Glass, C. K. Microglial cell origin and phenotypes in health and disease. Nature reviews. Immunology. 11,775-787, doi:10.1038/nri3086 (2011).
    • (2011) Nature reviews. Immunology. , vol.11 , pp. 775-787
    • Saijo, K.1    Glass, C.K.2
  • 6
    • 22244464662 scopus 로고    scopus 로고
    • ATP mediates rapid microglial response to local brain injury in vivo
    • doi:10.1038/nn1472
    • Davalos, D. et al. ATP mediates rapid microglial response to local brain injury in vivo. Nat. Neurosci. 8, 752-758, doi:10.1038/nn1472 (2005).
    • (2005) Nat. Neurosci. , vol.8 , pp. 752-758
    • Davalos, D.1
  • 7
    • 19744380563 scopus 로고    scopus 로고
    • Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo
    • doi:10.1126/science.1110647
    • Nimmerjahn, A., Kirchhoff, F. & Helmchen, F. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science. 308, 1314-1318, doi:10.1126/science.1110647 (2005).
    • (2005) Science. , vol.308 , pp. 1314-1318
    • Nimmerjahn, A.1    Kirchhoff, F.2    Helmchen, F.3
  • 8
    • 0029981976 scopus 로고    scopus 로고
    • Cytokine and free radical production by porcine microglia
    • Hu, S. et al. Cytokine and free radical production by porcine microglia. Clin. Immunol. Immunopathol. 78, 93-96 (1996).
    • (1996) Clin. Immunol. Immunopathol. , vol.78 , pp. 93-96
    • Hu, S.1
  • 9
    • 0033844166 scopus 로고    scopus 로고
    • Toll-like receptors: A growing family of immune receptors that are differentially expressed and regulated by different leukocytes
    • Muzio, M., Polentarutti, N., Bosisio, D., Prahladan, M. K. & Mantovani, A. Toll-like receptors: a growing family of immune receptors that are differentially expressed and regulated by different leukocytes. J. Leukocyte Biol. 67, 450-456 (2000).
    • (2000) J. Leukocyte Biol. , vol.67 , pp. 450-456
    • Muzio, M.1    Polentarutti, N.2    Bosisio, D.3    Prahladan, M.K.4    Mantovani, A.5
  • 10
    • 0142155221 scopus 로고    scopus 로고
    • Toll-like receptors and inflammation in the CNS
    • Lee, S. J. & Lee, S. Toll-like receptors and inflammation in the CNS. Curr. Drug Targets. Inflamm. Allergy. 1, 181-191 (2002).
    • (2002) Curr. Drug Targets. Inflamm. Allergy. , vol.1 , pp. 181-191
    • Lee, S.J.1    Lee, S.2
  • 11
    • 33845768784 scopus 로고    scopus 로고
    • Microglia-mediated neurotoxicity: Uncovering the molecular mechanisms
    • doi:10.1038/nrn2038
    • Block, M.L., Zecca, L. & Hong, J. S. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat. Rev. Neurosci. 8, 57-69, doi:10.1038/nrn2038 (2007).
    • (2007) Nat. Rev. Neurosci. , vol.8 , pp. 57-69
    • Block, M.L.1    Zecca, L.2    Hong, J.S.3
  • 12
    • 47849085872 scopus 로고    scopus 로고
    • The NALP3 inflammasome is involved in the innate immune response to amyloid-beta
    • doi:10.1038/ni.1636
    • Halle, A. et al. The NALP3 inflammasome is involved in the innate immune response to amyloid-beta. Nat. Immunol. 9, 857-865, doi:10.1038/ni.1636 (2008).
    • (2008) Nat. Immunol. , vol.9 , pp. 857-865
    • Halle, A.1
  • 13
    • 78649526394 scopus 로고    scopus 로고
    • Sterile inflammation: Sensing and reacting to damage
    • doi:10.1038/nri2873
    • Chen, G.Y. & Nunez, G. Sterile inflammation: sensing and reacting to damage. Nat. Rev. Immunol. 10, 826-837, doi:10.1038/nri2873 (2010).
    • (2010) Nat. Rev. Immunol. , vol.10 , pp. 826-837
    • Chen, G.Y.1    Nunez, G.2
  • 14
    • 77951800951 scopus 로고    scopus 로고
    • NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals
    • doi:10.1038/nature08938
    • Duewell, P. et al. NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals. Nature. 464, 1357-1361, doi:10.1038/nature08938 (2010).
    • (2010) Nature. , vol.464 , pp. 1357-1361
    • Duewell, P.1
  • 15
    • 75649087741 scopus 로고    scopus 로고
    • CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6heterodimer
    • doi:10.1038/ni.1836
    • Stewart, C. R. et al. CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6heterodimer. Nat. Immun. 11, 155-161, doi:10.1038/ni.1836 (2010).
    • (2010) Nat. Immun. , vol.11 , pp. 155-161
    • Stewart, C.R.1
  • 16
    • 84858677969 scopus 로고    scopus 로고
    • alpha-Synuclein Alters Toll-Like Receptor Expression
    • doi:10.3389/fnins.2011.00080
    • Beraud, D. et al. alpha-Synuclein Alters Toll-Like Receptor Expression. Front. Neurosci. 5, 80, doi:10.3389/fnins.2011.00080 (2011).
    • (2011) Front. Neurosci. , vol.5 , pp. 80
    • Beraud, D.1
  • 17
    • 0027351431 scopus 로고
    • Cytotoxicity of microglia
    • doi:10.1002/glia.440070117
    • Banati, R.B., Gehrmann, J., Schubert, P. & Kreutzberg, G. W. Cytotoxicity of microglia. Glia. 7, 111-118, doi:10.1002/glia.440070117 (1993).
    • (1993) Glia. , vol.7 , pp. 111-118
    • Banati, R.B.1    Gehrmann, J.2    Schubert, P.3    Kreutzberg, G.W.4
  • 18
    • 0035866060 scopus 로고    scopus 로고
    • beta-Amyloid stimulation of microglia and monocytes results in TNF alpha-dependent expression of induciblenitric oxide synthase and neuronal apoptosis
    • Combs, C.K., Karlo, J. C., Kao, S. C. & Landreth, G. E. beta-Amyloid stimulation of microglia and monocytes results in TNF alpha-dependent expression of induciblenitric oxide synthase and neuronal apoptosis. J. Neurosci. 21, 1179-1188 (2001).
    • (2001) J. Neurosci. , vol.21 , pp. 1179-1188
    • Combs, C.K.1    Karlo, J.C.2    Kao, S.C.3    Landreth, G.E.4
  • 19
    • 33748447845 scopus 로고    scopus 로고
    • Microglia, major player in the brain inflammation: Their roles in the pathogenesis of Parkinson's disease
    • Kim, Y. S. & Joh, T. H. Microglia, major player in the brain inflammation: their roles in the pathogenesis of Parkinson's disease. Exp. Mol. Med. 38, 333-347 (2006).
    • (2006) Exp. Mol. Med. , vol.38 , pp. 333-347
    • Kim, Y.S.1    Joh, T.H.2
  • 21
    • 0036321329 scopus 로고    scopus 로고
    • Ceramide activates microglia to enhance the production/secretion of brain-derived neurotrophic factor (BDNF) without induction of deleterious factors in vitro
    • Nakajima, K., Tohyama, Y., Kohsaka, S. & Kurihara, T. Ceramide activates microglia to enhance the production/secretion of brain-derived neurotrophic factor (BDNF) without induction of deleterious factors in vitro. J. Neurochem. 80, 697-705 (2002).
    • (2002) J. Neurochem. , vol.80 , pp. 697-705
    • Nakajima, K.1    Tohyama, Y.2    Kohsaka, S.3    Kurihara, T.4
  • 22
    • 33644938437 scopus 로고    scopus 로고
    • Nonparticipation of nuclear factor kappa B (NFkappaB) in the signaling cascade of c-JunN-terminal kinase (JNK)-and p38 mitogen-activated protein kinase (p38MAPK)-dependent tumor necrosis factor alpha (TNFalpha) induction in lipopolysaccharide (LPS)-stimulated microglia
    • doi:10.1016/j.brainres.2005.12.043
    • Uesugi, M., Nakajima, K., Tohyama, Y., Kohsaka, S. & Kurihara, T. Nonparticipation of nuclear factor kappa B (NFkappaB) in the signaling cascade of c-JunN-terminal kinase (JNK)-and p38 mitogen-activated protein kinase (p38MAPK)-dependent tumor necrosis factor alpha (TNFalpha) induction in lipopolysaccharide (LPS)-stimulated microglia. Brain Res. 1073-1074, 48-59, doi:10.1016/j.brainres.2005.12.043 (2006).
    • (2006) Brain Res. , vol.1073-1074 , pp. 48-59
    • Uesugi, M.1    Nakajima, K.2    Tohyama, Y.3    Kohsaka, S.4    Kurihara, T.5
  • 23
    • 84874654334 scopus 로고    scopus 로고
    • Microglial Activation and Antioxidant Responses Induced by the Parkinson's Disease Protein alpha-Synuclein
    • doi:10.1007/s11481-012-9401-0
    • Beraud, D. et al. Microglial Activation and Antioxidant Responses Induced by the Parkinson's Disease Protein alpha-Synuclein. J. Neuroimmun. Pharmacol. 8, 94-117, doi:10.1007/s11481-012-9401-0 (2013).
    • (2013) J. Neuroimmun. Pharmacol. , vol.8 , pp. 94-117
    • Beraud, D.1
  • 24
    • 51449099473 scopus 로고    scopus 로고
    • Synuclein activates microglia in a model of Parkinson's disease
    • doi:10.1016/j.neurobiolaging.2007.04.006
    • Su, X. et al. Synuclein activates microglia in a model of Parkinson's disease. Neurobiol. Aging. 29, 1690-1701, doi:10.1016/j.neurobiolaging.2007.04.006 (2008).
    • (2008) Neurobiol. Aging. , vol.29 , pp. 1690-1701
    • Su, X.1
  • 25
    • 0031985513 scopus 로고    scopus 로고
    • Microglia as effector cells in brain damage and repair: Focus on prostanoids and nitric oxide
    • Minghetti, L. & Levi, G. Microglia as effector cells in brain damage and repair: focus on prostanoids and nitric oxide. Prog. Neurobiol. 54, 99-125 (1998).
    • (1998) Prog. Neurobiol. , vol.54 , pp. 99-125
    • Minghetti, L.1    Levi, G.2
  • 26
    • 0034052904 scopus 로고    scopus 로고
    • Glial cells and Parkinson's disease
    • Hirsch, E.C. Glial cells and Parkinson's disease. J. Neurol. 247 Suppl 2, II58-62 (2000).
    • (2000) J. Neurol. , vol.247 , Issue.SUPPL. 2
    • Hirsch, E.C.1
  • 27
    • 0036272473 scopus 로고    scopus 로고
    • Role of nitric oxide in inflammation-mediated neurodegeneration
    • Liu, B. et al. Role of nitric oxide in inflammation-mediated neurodegeneration. Ann. NY Acad. Sci. 962, 318-331 (2002).
    • (2002) Ann. NY Acad. Sci. , vol.962 , pp. 318-331
    • Liu, B.1
  • 28
    • 65649099379 scopus 로고    scopus 로고
    • Modulation of microglialinnate immunity in Alzheimer's disease by activation of peroxisome proliferator-activated receptor gamma
    • Shie, F.S., Nivison, M., Hsu, P. C. & Montine, T. J. Modulation of microglialinnate immunity in Alzheimer's disease by activation of peroxisome proliferator-activated receptor gamma. Curr. Med. Chem. 16, 643-651 (2009).
    • (2009) Curr. Med. Chem. , vol.16 , pp. 643-651
    • Shie, F.S.1    Nivison, M.2    Hsu, P.C.3    Montine, T.J.4
  • 29
    • 34547380204 scopus 로고    scopus 로고
    • Neuroinflammation in Alzheimer's disease and Parkinson's disease: Are microglia pathogenic in either disorder?
    • doi:10.1016/S0074-7742(07)82012-5
    • Rogers, J., Mastroeni, D., Leonard, B., Joyce, J. & Grover, A. Neuroinflammation in Alzheimer's disease and Parkinson's disease: are microglia pathogenic in either disorder? Int. Rev. Neurobiol. 82, 235-246, doi:10.1016/S0074-7742(07)82012-5 (2007).
    • (2007) Int. Rev. Neurobiol. , vol.82 , pp. 235-246
    • Rogers, J.1    Mastroeni, D.2    Leonard, B.3    Joyce, J.4    Grover, A.5
  • 30
    • 34248673126 scopus 로고    scopus 로고
    • Isolation of murinemicroglial cells for RNA analysis or flow cytometry
    • doi:10.1038/nprot.2006.327
    • Cardona, A. E., Huang, D., Sasse, M. E. & Ransohoff, R. M. Isolation of murinemicroglial cells for RNA analysis or flow cytometry. Nat. Protoc. 1, 1947-1951, doi:10.1038/nprot.2006.327 (2006).
    • (2006) Nat. Protoc. , vol.1 , pp. 1947-1951
    • Cardona, A.E.1    Huang, D.2    Sasse, M.E.3    Ransohoff, R.M.4
  • 31
    • 0029002659 scopus 로고
    • Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigenpresentation to myelin basic protein-reactive CD4+ T cells compared
    • Ford, A.L., Goodsall, A. L., Hickey, W. F. & Sedgwick, J. D. Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigenpresentation to myelin basic protein-reactive CD4+ T cells compared. J. Immunol. 154, 4309-4321 (1995).
    • (1995) J. Immunol. , vol.154 , pp. 4309-4321
    • Ford, A.L.1    Goodsall, A.L.2    Hickey, W.F.3    Sedgwick, J.D.4
  • 32
    • 0030591586 scopus 로고    scopus 로고
    • Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression
    • Ford, A.L., Foulcher, E., Goodsall, A. L. & Sedgwick, J. D. Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression. J. Immunol. Methods. 194, 71-75 (1996).
    • (1996) J. Immunol. Methods. , vol.194 , pp. 71-75
    • Ford, A.L.1    Foulcher, E.2    Goodsall, A.L.3    Sedgwick, J.D.4
  • 33
    • 80355125448 scopus 로고    scopus 로고
    • Isolation of brain and spinal cord mononuclear cells using percoll gradients
    • doi:10.3791/2348
    • Pino, P.A. & Cardona, A. E. Isolation of brain and spinal cord mononuclear cells using percoll gradients. J. Vis. Exp. doi:10.3791/2348 (2011).
    • (2011) J. Vis. Exp.
    • Pino, P.A.1    Cardona, A.E.2
  • 34
    • 84868256937 scopus 로고    scopus 로고
    • Detection of microRNAs in microglia by real-time PCR in normal CNS and during neuroinflammation
    • doi:10.3791/4097
    • Veremeyko, T., Starossom, S. C., Weiner, H. L. & Ponomarev, E. D. Detection of microRNAs in microglia by real-time PCR in normal CNS and during neuroinflammation. J. Vis. Exp. doi:10.3791/4097 (2012).
    • (2012) J. Vis. Exp.
    • Veremeyko, T.1    Starossom, S.C.2    Weiner, H.L.3    Ponomarev, E.D.4
  • 35
    • 70349917814 scopus 로고    scopus 로고
    • Mutant alpha-synuclein overexpression mediates early proinflammatory activity
    • doi:10.1007/s12640-009-9053-x
    • Su, X., Federoff, H. J. & Maguire-Zeiss, K. A. Mutant alpha-synuclein overexpression mediates early proinflammatory activity. Neurotox. Res. 16, 238-254, doi:10.1007/s12640-009-9053-x (2009).
    • (2009) Neurotox. Res. , vol.16 , pp. 238-254
    • Su, X.1    Federoff, H.J.2    Maguire-Zeiss, K.A.3
  • 36
    • 0034028817 scopus 로고    scopus 로고
    • Analysis of fractalkine receptorCX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion
    • Jung, S. et al. Analysis of fractalkine receptorCX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol. Cell. Biol.20, 4106-4114 (2000).
    • (2000) Mol. Cell. Biol. , vol.20 , pp. 4106-4114
    • Jung, S.1
  • 37
    • 0030723388 scopus 로고    scopus 로고
    • Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion
    • Imai, T. et al. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell.91,521-530 (1997).
    • (1997) Cell. , vol.91 , pp. 521-530
    • Imai, T.1
  • 38
    • 33745573660 scopus 로고    scopus 로고
    • Control of microglial neurotoxicity by the fractalkine receptor
    • doi:10.1038/nn1715
    • Cardona, A. E. et al. Control of microglial neurotoxicity by the fractalkine receptor. Nat. Neurosci. 9, 917-924, doi:10.1038/nn1715 (2006).
    • (2006) Nat. Neurosci. , vol.9 , pp. 917-924
    • Cardona, A.E.1
  • 40
    • 33644511746 scopus 로고    scopus 로고
    • Rapid isolation of highly enriched and quiescent microglia from adult rat hippocampus:Immunophenotypic and functional characteristics
    • doi:10.1016/j.jneumeth.2005.06.026
    • Frank, M.G., Wieseler-Frank, J. L., Watkins, L. R. & Maier, S. F. Rapid isolation of highly enriched and quiescent microglia from adult rat hippocampus:immunophenotypic and functional characteristics. J. Neurosci. Methods. 151, 121-130, doi:10.1016/j.jneumeth.2005.06.026 (2006).
    • (2006) J. Neurosci. Methods. , vol.151 , pp. 121-130
    • Frank, M.G.1    Wieseler-Frank, J.L.2    Watkins, L.R.3    Maier, S.F.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.