Neuroscience: Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo

Science

Volumn 308, Issue 5726, 2005, Pages 1314-1318

  Nimmerjahn, Axel ^a   Kirchhoff, Frank ^b   Helmchen, Fritjof ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b MAX PLANCK INSTITUTE OF EXPERIMENTAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; BRAIN; CELL CULTURE; CELLS; DISEASES; IMAGING TECHNIQUES; IMMUNOLOGY;

BRAIN DAMAGE; MICROGLIAL CELLS; NEOCORTEX; TWO-PHOTON IMAGING;

NEUROLOGY;

NEUROLOGY;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD BRAIN BARRIER; BRAIN INJURY; BRAIN REGION; CELL MOTILITY; IMMUNE SYSTEM; IN VIVO STUDY; MAMMAL; MICROENVIRONMENT; MICROGLIA; MOUSE; NEOCORTEX; NERVE CELL STIMULATION; NONHUMAN; PRIORITY JOURNAL; ANIMAL; ASTROCYTE; CAPILLARY; CELL MOTION; CELL SURFACE; CYTOLOGY; FLUORESCENCE MICROSCOPY; INJURY; LASER; PATHOPHYSIOLOGY; PHYSIOLOGY; PSEUDOPODIUM; TRANSGENIC MOUSE; ULTRASTRUCTURE;

ANIMALIA; MAMMALIA;

4 AMINOBUTYRIC ACID RECEPTOR BLOCKING AGENT; BICUCULLINE; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; LIPOPOLYSACCHARIDE; SODIUM CHANNEL BLOCKING AGENT; TETRODOTOXIN;

ANIMALS; ASTROCYTES; BICUCULLINE; BLOOD-BRAIN BARRIER; BRAIN INJURIES; CAPILLARIES; CELL MOVEMENT; CELL SURFACE EXTENSIONS; GABA ANTAGONISTS; GREEN FLUORESCENT PROTEINS; LASERS; LIPOPOLYSACCHARIDES; MICE; MICE, TRANSGENIC; MICROGLIA; MICROSCOPY, FLUORESCENCE; NEOCORTEX; PSEUDOPODIA; SODIUM CHANNEL BLOCKERS; TETRODOTOXIN;

EID: 19744380563     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1110647     Document Type: Article

References (19)

1. G. W. Kreutzberg. Trends Neurosci. 19, 312 (1996).
2. G. Stoll, S. Jander, Prog. Neurobiol. 58, 233 (1999).
3. W. J. Streit, Glia 40, 133 (2002).
4. W. J. Streit, J. Neurosci. Res. 77, 1 (2004).
5. C. Nolte, T. Moller, T. Walter, H. Kettenmann, Neuroscience 73, 1091 (1996).
6. N. Stence, M. Waite, M. E. Dailey, Glia 33, 256 (2001).
7. W. Denk, K. Svoboda, Neuron 18, 351 (1997).
8. S. Jung et al., Mol. Cell. Biol. 20, 4106 (2000).
9. Materials and methods are available as supporting material on Science Online.
10. A. Lehmenkuhler, E. Sykova, J. Svoboda, K. Zilles, C. Nicholson, Neuroscience 55, 339 (1993).
11. A. Nimmerjahn, F. Kirchhoft, J. N. D. Kerr, F. Helmchen, Nat. Methods 1, 31 (2004).
12. D. van Rossum, U. K. Hanisch, Metab. Brain Dis. 19, 393 (2004).
13. J. Grutzendler, N. Kasthuri, W. B. Can, Nature 420, 812 (2002).
14. J. T. Trachtenberg et al., Nature 420, 788 (2002).
15. J. Hirrlinger, S. Hulsmann, F. Kirchhoff, Eur. J. Neurosci. 20, 2235 (2004).
16. G. Raivich et al., Brain Res. Rev. 30, 77 (1999).
17. F. Capani, M. H. Ellisman, M. E. Martone, Brain Res. 923, 1 (2001).
18. F. Vilhardt, Int. J. Biochem. Cell Biol. 37, 17 (2005).
19. We thank S. Erdogan for help with the analysis, J. N. D. Kerr and G. W. Kreutzberg for comments on the manuscript, S. Jung and D. R. Littman for providing the green fluorescent microglia mouse line, and B. Sakmann for generous support. This work was supported by a predoctoral fellowship of the Boehringer Ingelheim Fonds to A.N.