Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) dunal in shake-flask culture and bioreactor

PLoS ONE

Volumn 9, Issue 8, 2014, Pages

  Sivanandhan, Ganeshan ^a   Selvaraj, Natesan ^b   Ganapathi, Andy ^a   Manickavasagam, Markandan ^a  

^a BHARATHIDASAN UNIVERSITY   (India)

^b Govt Arts College   (India)

Author keywords

[No Author keywords available]

Indexed keywords

12 DEOXY WITHANSTRAMONOLIDE; ALUMINUM CHLORIDE; CADMIUM CHLORIDE; CHITOSAN; CHOLESTEROL; MEVALONIC ACID; SQUALENE; UNCLASSIFIED DRUG; WITHAFERIN A; WITHANIA SOMNIFERA EXTRACT; WITHANOLIDE; WITHANOLIDE A; WITHANOLIDE B; WITHANONE; WITHANOSIDE IV; WITHANOSIDE V; ALUMINUM DERIVATIVE; CHLORIDE; CULTURE MEDIUM; GLUTAMINE; PICLORAM; SUCROSE;

ARTICLE; BIOMASS; BIOREACTOR; BIOSYNTHESIS; CELL CULTURE; CELL CULTURE EQUIPMENT; CELL SUSPENSION; CHEMICAL PROCEDURES; CONTROLLED STUDY; DRY WEIGHT; ELICITATION; NONHUMAN; PLANT ROOT; SHAKE FLASK CULTURE; WITHANIA SOMNIFERA; CHEMISTRY; CLASSIFICATION; CULTURE MEDIUM; CULTURE TECHNIQUE; DRUG EFFECTS; METABOLISM; WITHANIA;

ALUMINUM COMPOUNDS; BIOREACTORS; CADMIUM CHLORIDE; CELL CULTURE TECHNIQUES; CHITOSAN; CHLORIDES; CHOLESTEROL; CULTURE MEDIA; GLUTAMINE; MEVALONIC ACID; PICLORAM; SQUALENE; SUCROSE; WITHANIA; WITHANOLIDES;

EID: 84905493044     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0104005     Document Type: Article

References (48)

1. Cardellina JH (2002) Challenges and opportunities confronting the botanical dietary supplement industry. J Nat Prod 65:1073-1084.
2. Dörnenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzym Microb Technol 17:674-684.
3. Singh M, Chaturvedi R (2012) Evaluation of nutrient uptake and physical parameters on cell biomass growth and production of spilanthol in suspension cultures of Spilanthes acmella Murr. Bioprocess Biosyst Eng 35:943-951.
4. Sivanandhan G, Mariashibu TS, Arun M, Rajesh M, Kasthurirengan S, et al. (2011) The effect of polyamines on the efficiency of multiplication and rooting of Withania somnifera (L.) Dunal and content of some withanolides in obtained plants. Acta Physiol Plant 33:2279-2288.
5. Sivanandhan G, Arun M, Mayavan S, Rajesh M, Mariashibu TS, et al. (2012) Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal. Ind Crop Prod 37:124-129.
6. Kuboyama T, Tohda C, Komatsu K (2005) Neuritic regeneration and synaptic reconstruction induced by Withanolide-A. Br J Pharmacol 144:961-971. (Pubitemid 40593022)
7. Malik F, Singh J, Khajuria A, Suri KA, Satti NK, et al. (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A solicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80:1525-1538. (Pubitemid 46399749)
8. Khan SF, Malik Suri KA, Singh J (2009) Molecular insight into the immune upregulatory properties of the leaf extract of Ashwagandha and identification of Th1 immunostimulatory chemical entity. Vaccine 27:6080-6087.
9. Malik F, Kumar A, Bhushan S, Khan S, Bhatia A, et al. (2007) Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic cell death of human myeloid leukemia HL-60 cells by a dietary compound withaferin A with concomitant protection by N-acetyl cysteine. Apoptosis 12:2115-2133. (Pubitemid 47498235)
10. Ku SK, Bae JS (2014) Antiplatelet, anticoagulant, and profibrinolytic activities of withaferin A. Vascul Pharmacol 60: 120-126.
11. Berghe WV, Sabbe L, Kaileh M, Haegeman G, Heyninek K (2012) Molecular insight in the multifunctional activities of Withaferin A. Biochem Pharmacol 84:1282-1291.
12. Mohan R, Hammers HJ, Bargagna MP, Zhan XH, Herbstritt CJ, et al. (2004) Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7:115-122. (Pubitemid 39491728)
13. Mishra LC, Singh BB, Dagenais S (2000) Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review. Alternat Med Rev 5:334-346. (Pubitemid 30660827)
14. Sharada M, Ahuja A, Vij SP (2008) Applications of biotechnology in Indian ginseng (ashwagandha): progress and prospects. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology and its applications. I. K. International Pvt Ltd, New Delhi, pp 645-667.
15. Sivanandhan G, Arun M, Mayavan S, Rajesh M, Jeyaraj M, et al. (2012) Optimization of elicitation conditions with methyl jasmonate and salicylic acid to improve the productivity of withanolides in the adventitious root culture of Withania somnifera (L.) Dunal. Appl Biochem Biotechnol 168: 681-696.
16. Sangwan RS, Chaurasia ND, Misra LN, Lal P, Uniyal GC, et al. (2007) Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Curr Sci 86:461-465. (Pubitemid 39160291)
17. Zhang W, Curtin C, Franco C (2002) Towards manipulation of postbiosynthetic events in secondary metabolism of plant cell cultures. Enzyme Microb Technol 30:688-696. (Pubitemid 34603321)
18. Sabir F, Sangwan RS, Kumar R, Sangwan NS (2012) Salt stress-induced responses in growth and metabolism in callus cultures and differentiating in vitro shoots of Indian ginseng (Withania somnifera Dunal). J Plant Growth Regul 31:537-548
19. Mishra B, Sangwan RS, Mishra S, Jadaun JS, Sabir F, et al. (2014) Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of Ashwagandha (Withania somnifera Dunal). Protoplasma (in press, doi: DOI 10.1007/s00709-014-0613-4)
20. Mustafa NR, de Winter W, van Iren F, Verpoorte R (2011) Initiation, growth and cryopreservation of plant cell suspension cultures. Nat Protoc 6:715-742.
21. Verpoorte R, van der Heijden R, ten Hoopen HJG, Memelink J (1999) Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnol Lett 21:467-479. (Pubitemid 29349151)
22. Rao RS, Ravishankar GA (2002) Plant cell cultures, chemical factories of secondary metabolites. Biotechnol Adv 20: 1-53.
23. Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839-85.
24. Zhang W, Furusaki S (1999) Production of anthocyanins by plant cell cultures. Biotechnol Bioprocess Eng 4:231-252.
25. Dias LLC, Santa-Catarina C, Ribeiro DM, Barros RS, Floh EIS, et al. (2009) Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor. Plant Cell Tiss Organ Cult 99:199-208.
26. Rhee HS, Cho HY, Son SY, Yoon SYH, Park JM (2010) Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation. Plant Cell Tiss Organ Cult 101:295-302.
27. Lucumi E, Vera A, Hallard D, van der Heijden R, Verpoorte R (2002) Alkaloid formation in cell suspension cultures of Tabernaemontana elegans after feeding of tryptamine and loganin or secologanin. Plant Cell Tiss Org Cult 68:293-299. (Pubitemid 34166157)
28. Sivanandhan G, Kapil Dev G, Jeyaraj M, Rajesh M, Muthuselvam M, et al. (2013) A promising approach on biomass accumulation and withanolides production in cell suspension culture of Withania somnifera (L.) Dunal. Protoplasma 250:885-898.
29. Sivanandhan G, Kapil Dev G, Jeyaraj M, Rajesh M, Manickavasagam M, et al. (2013) Increased production of withanolide A, withanone and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid. Plant Cell Tiss Organ Cult 114: 121-129.
30. Ciddi V (2006) Withaferin A from cell cultures of Withania somnifera. Indian J Pharm Sci 68:490-492.
31. Baldi A, Singh D, Dixit VK (2008) Dual elicitation for improved production of withaferin A by cell suspension cultures of Withania somnifera . Appl Biochem Biotechnol 151:556-564.
32. Nagella P, Murthy HN (2010) Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A. Bioresour Technol 101:6735-6739.
33. Nagella P, Murthy HN (2011) Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tissue Organ Cult 104:119-124.
34. Sabir F, Sangwan RS, Singh J, Misra LN, Pathak N, et al. (2011) Biotransformation of withanolides by cell suspension cultures of Withania somnifera (Dunal.). Plant Biotech Rep 5:127-134.
35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
36. Ouyang J, Wang XD, Zhao B, Wang YC (2005). Enhanced production of phenylethanoid glycosides by precursor feeding to cell culture of Cistanche deserticola. Process Biochem. 40:3480-3484. (Pubitemid 41323928)
37. Liu JY, Guo ZG, Zeng ZL (2007) Improved accumulation of phenylethanoid glycosides by precursor feeding to suspension culture of Cistanche salsa. Biochem Eng J 33:88-93.
38. Baldi A, Dixit VK (2008) Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua . Bioresour Tech 99:4609-4614. (Pubitemid 351503806)
39. Zhang CH, Fevereiro PS, He G, Chen Z (2007) Enhanced paclitaxel productivity and release capacity of Taxus chinensis cell suspension cultures adapted to chitosan. Plant Sci 172:158-163 (Pubitemid 44709251)
40. Krzyzanowska J, Czubacka A, Pecio L, Przybys M, Doroszewska T, et al. (2012) The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha6piperita cell suspension cultures. Plant Cell Tiss Organ Cult 108:73-81.
41. Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci 172:861-875. (Pubitemid 46517729)
42. Zhao J, Davis TLC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283-333. (Pubitemid 40560881)
43. Zhao JL, Zhou LG, Wu JY (2010) Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Appl Microbiol Biotechnol 87:137-144.
44. Flores-Sanchez IJ, Ortega-Lopez J, Montes-Horcasitas MC, Ramos-Valdivia AC (2002) Biosynthesis of sterols and triterpenes in cell suspension cultures of Uncaria tomentosa. Plant Cell Physiol 43:1502-1509. (Pubitemid 36100987)
45. Chaurasia ND, Sangwan NS, Sabir F, Misra L, Sangwan RS (2012) Withanolide biosynthesis recruits both mevalonate and DOXP pathways of isoprenogenesis in Ashwagandha Withania somnifera L.(Dunal). Plant Cell Rep 31: 1889-1897.
46. Sangwan RS, Chaurasiya ND, Lal P, Misra L, Tuli R, et al. (2008) Withanolide A is inherently de novo biosynthesized in roots of the medicinal plant Ashwagandha (Withania somnifera). Physiol Plant 133:278-287. (Pubitemid 351641088)
47. Patil JT, Ahire ML, Nitnaware KM, Panda S, Bhatt VP, et al. (2013) In vitro propagation and production of cardiotonic glycosides in shoot cultures of Digitalis purpurea L. by elicitation and precursor feeding. Appl Microbiol Biotechnol 97:2379-2393.
48. Zhao J, Zhu W, Hu Q (2001) Effects of stress factors, bioregulators and synthetic precursor on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus . Appl Microbiol Biotechnol 55:693-698. (Pubitemid 32661577)