Salt Stress-induced Responses in Growth and Metabolism in Callus Cultures and Differentiating In Vitro Shoots of Indian Ginseng (Withania somnifera Dunal)

Journal of Plant Growth Regulation

Volumn 31, Issue 4, 2012, Pages 537-548

  Sabir, Farzana ^a   Sangwan, Rajender S ^a   Kumar, Ritesh ^a   Sangwan, Neelam S ^a  

^a CSIR CENTRAL INSTITUTE OF MEDICINAL AND AROMATIC PLANTS   (India)

Author keywords

Antioxidant enzymes; Ashwagandha; Calcium chloride; In vitro callus and shoot cultures; Indian ginseng; Potassium nitrate; Sodium chloride and sodium nitrate

Indexed keywords

PANAX GINSENG; WITHANIA SOMNIFERA;

EID: 84868339242     PISSN: 07217595     EISSN: 14358107     Source Type: Journal    
DOI: 10.1007/s00344-012-9264-x     Document Type: Article

References (45)

1. Agastian P, Kingsley SJ, Vivekanandan M (2000) Effect of salinity on photosynthesis and biochemical characteristics in mulberry genotypes. Photosynthetica 38: 287-290.
2. Alia P, Saradhi PP, Mohanty P (1991) Proline enhances primary photochemical activities in isolated thylakoid membranes of Brassica juncea by arresting photo inhibitory damage. Biochem Biophys Res Commun 181: 1238-1244.
3. Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99: 191-203.
4. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39: 205-207.
5. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287.
6. Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7: 1099-1111.
7. Chance B, Maehly AC (1955) Assay of catalases and peroxidases. Methods Enzymol 2: 764-775.
8. Chaves MM, Oliveira MM (2004) Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J Exp Bot 55: 2365-2384.
9. Cheng AX, Lou YG, Mao YB, Lu S, Wang LJ, Chen XY (2007) Plants terpenoids: biosynthesis and ecological functions. J Integr Plant Biol 49: 179-186.
10. De Abreu IN, Mazzafera P (2005) Effect of water and temperature stress on the content of active constituents of Hypericum brasilienne Choisy. Plant Physiol Biochem 43: 241-248.
11. Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135: 1-9.
12. Elkahoui S, Hernandez JA, Abdelly C, Ghrir R, Limam F (2005) Effects of salt on lipid peroxidation and antioxidant enzyme activities of Catharanthus roseus suspension cells. Plant Sci 168: 607-613.
13. Erturk U, Sivritepe N, Yerlikaya C, Bor M, Ozdemir F, Turkan I (2007) Responses of the cherry rootstock to salinity in vitro. Biol Plant 51: 597-600.
14. Hare P, Cress WA, van Staden J (1998) Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ 21: 535-553.
15. Kovacik J, Klejdus B, Hedbavny J, Backor M (2009) Salicylic acid alleviates NaCl-induced changes in the metabolism of Matricaria chamomilla plants. Ecotoxicology 18: 544-554.
16. Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C (2007) Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiol Biochem 45: 244-249.
17. Kumar KB, Khan PA (1982) Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Ind J Exp Bot 20: 412-416.
18. Kwon SY, Jeong YJ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerance of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant Cell Environ 25: 873-882.
19. Lattanzio V, Cardinali A, Ruta C, Fortunato IM, Lattanzio VMT, Linsalata V, Cicco N (2009) Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress. Environ Exp Bot 65: 54-62.
20. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11: 591-592.
21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
22. Mattioli R, Costantino P, Trovato M (2009) Proline accumulation in plants, not only stress. Plant Signal Behav 4: 1016-1018.
23. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7: 405-410.
24. Molassiotis AN, Sotiropoulos T, Tanou G, Kofidis G, Diamantidis G, Therios Ι (2006) Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol. Biol Plant 50: 331-338.
25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15: 473-497.
26. Muthukumarasamy M, Gupta SD, Pannerselvam R (2000) Enhancement of peroxidase, polyphenol oxidase and superoxide dismutase activities by tridimefon in NaCl stressed Raphanus sativus L. Biol Plant 43: 317-320.
27. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867-880.
28. Navarro JM, Flores P, Garrido C, Martinez V (2006) Changes in the contents of antioxidant compounds in pepper fruits at ripening stages, as affected by salinity. Food Chem 96: 66-73.
29. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 163: 375-380.
30. Oueslati S, Bouraoui NK, Attia H, Rabhi M, Ksouri R, Lachaal M (2009) Physiological and antioxidant responses of Mentha pulegium (Pennyroyal) to salt stress. Acta Physiol Plant 32: 289-296.
31. Panda SK, Upadhyay RK (2003) Salt stress injury induces oxidative alterations and antioxidative defense in the roots of Lemna minor. Biol Plant 48: 249-253.
32. Sabir F, Sangwan NS, Chaurasiya ND, Misra LN, Sangwan RS (2008) In vitro withanolide production by Withania somnifera L. cultures. Z Naturforsch 63: 409-412.
33. Sam O, Ramirez C, Coronado MJ, Testillano PS, Risueno MC (2003) Changes in tomato leaves induced by NaCl stress: leaf organization and cell ultrastructure. Biol Plant 47: 361-366.
34. Sangwan NS, Farooqi AHA, Sangwan RS (1994) Effect of drought on growth and essential oil metabolism in lemongrass species. New Phytol 128: 173-179.
35. Sangwan NS, Yadav U, Sangwan RS (2003) Genetic diversity among elite varieties of the aromatic grasses, Cymbopogon martini. Euphytica 130: 117-130.
36. Sangwan RS, Chaurasiya ND, Misra LN, Lal P, Uniyal GC, Sharma R, Sangwan NS, Suri KA, Qazi GN, Tuli R (2004) Phytochemical variability in commercial herbal products and preparations of Withania somnifera (ashwagandha). Curr Sci 86: 461-465.
37. Shewfelt RL, Purvis AC (1995) Towards a comprehensive model for lipid peroxidation in plant tissue disorders. Hort Sci 30: 213-221.
38. Shimoni M (1994) A method for activity staining of peroxidase and β-1, 3-glucanase isozymes in polyacrylamide electrophoresis gels. Anal Biochem 220: 36-38.
39. Smith IK, Vierhelle TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenate using 5, 5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175: 408-413.
40. Vijayan K, Chakraborti SP, Ghosh PD (2003) In vitro screening of mulberry (Morus spp.) for salinity tolerance. Plant Cell Rep 22: 350-357.
41. Wang B, Lüttge U, Ratajczak R (2004) Specific regulation of SOD isoforms by NaCl and osmotic stress in leaves of the C3 halophyte Suaeda salsa L. J Plant Physiol 161: 285-289.
42. Woodward AJ, Bennett IJ (2005) The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tissue Organ Cult 82: 189-200.
43. Xiong L, Zhu JK (2002) Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell Environ 25: 131-139.
44. Zhao X, Tan HJ, Liu YB, Li XR, Chen GX (2009) Effect of salt stress on growth and osmotic regulation in Thellungiella and Arabidopsis callus. Plant Cell Tissue Organ Cult 98: 97-103.
45. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6: 66-71.