A novel cell line from spontaneously immortalized murine microglia

Journal of Neuroscience Methods

Volumn 233, Issue , 2014, Pages 187-198

  Nagamoto Combs, Kumi ^a   Kulas, Joshua ^a   Combs, Colin K ^a  

^a UNIVERSITY OF NORTH DAKOTA SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

Beta amyloid; Inflammation; Lipopolysaccharide; M1 M2 phenotype; Microglia; Phagocytosis

Indexed keywords

AMYLOID BETA PROTEIN[1-42]; ARGINASE 1; CD68 ANTIGEN; CYCLOOXYGENASE 2; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERLEUKIN 4; LIPOPOLYSACCHARIDE; TOLL LIKE RECEPTOR 4; TUMOR NECROSIS FACTOR ALPHA; ACTIN BINDING PROTEIN; AIF1 PROTEIN, MOUSE; AMYLOID BETA PROTEIN; AMYLOID BETA-PROTEIN (1-42); ARG1 PROTEIN, MOUSE; ARGINASE; CALCIUM BINDING PROTEIN; CD68 PROTEIN, MOUSE; DIFFERENTIATION ANTIGEN; ESCHERICHIA COLI PROTEIN; LEUKOCYTE ANTIGEN; NITRIC OXIDE; NOS2 PROTEIN, MOUSE; PEPTIDE FRAGMENT; PROTEIN TYROSINE KINASE; PTGS2 PROTEIN, MOUSE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CELL CLONE; CELL ISOLATION; CONTROLLED STUDY; CYTOKINE RELEASE; IMMORTALIZED CELL LINE; IMMUNOREACTIVITY; IN VITRO STUDY; MICROGLIA; MOUSE; NEWBORN; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; ANIMAL; C57BL MOUSE; CELL LINE; CYTOLOGY; DRUG EFFECTS; METABOLISM; PHYSIOLOGY;

AMYLOID BETA-PEPTIDES; ANIMALS; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; ARGINASE; CALCIUM-BINDING PROTEINS; CELL LINE; CYCLOOXYGENASE 2; ESCHERICHIA COLI PROTEINS; INTERLEUKIN-4; LIPOPOLYSACCHARIDES; MICE, INBRED C57BL; MICROFILAMENT PROTEINS; MICROGLIA; NF-KAPPA B; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE II; PEPTIDE FRAGMENTS; PHAGOCYTOSIS; PROTEIN-TYROSINE KINASES; SIGNAL TRANSDUCTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84903904211     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2014.05.021     Document Type: Article

References (48)

1. Abd-El-Basset E., Fedoroff S. Effect of bacterial wall lipopolysaccharide (LPS) on morphology, motility, and cytoskeletal organization of microglia in cultures. J Neurosci Res 1995, 41:222-237.
2. Alliot F., Marty M.C., Cambier D., Pessac B. A spontaneously immortalized mouse microglial cell line expressing CD4. Brain Res Dev Brain Res 1996, 95:140-143.
3. Bachstetter A.D., Xing B., de Almeida L., Dimayuga E.R., Watterson D.M., Van Eldik L.J. Microglial p38alpha MAPK is a key regulator of proinflammatory cytokine up-regulation induced by toll-like receptor (TLR) ligands or beta-amyloid (Abeta). J Neuroinflamm 2011, 8:79.
4. Bhat N.R., Zhang P., Lee J.C., Hogan E.L. Extracellular signal-regulated kinase and p38 subgroups of mitogen-activated protein kinases regulate inducible nitric oxide synthase and tumor necrosis factor-alpha gene expression in endotoxin-stimulated primary glial cultures. J Neurosci 1998, 18:1633-1641.
5. Blasi E., Barluzzi R., Bocchini V., Mazzolla R., Bistoni F. Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol 1990, 27:229-237.
6. Boje K.M., Arora P.K. Microglial-produced nitric oxide and reactive nitrogen oxides mediate neuronal cell death. Brain Res 1992, 587:250-256.
7. Briers T.W., Desmaretz C., Vanmechelen E. Generation and characterization of mouse microglial cell lines. J Neuroimmunol 1994, 52:153-164.
8. Chao C.C., Hu S., Molitor T.W., Shaskan E.G., Peterson P.K. Activated microglia mediate neuronal cell injury via a nitric oxide mechanism. J Immunol 1992, 149:2736-2741.
9. Cheepsunthorn P., Radov L., Menzies S., Reid J., Connor J.R. Characterization of a novel brain-derived microglial cell line isolated from neonatal rat brain. Glia 2001, 35:53-62.
10. Chhor V., Le Charpentier T., Lebon S., Ore M.V., Celador I.L., Josserand J., et al. Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro. Brain Behav Immun 2013, 32:70-85.
11. Combs C.K., Johnson D.E., Cannady S.B., Lehman T.M., Landreth G.E. Identification of microglial signal transduction pathways mediating a neurotoxic response to amyloidogenic fragments of beta-amyloid and prion proteins. J Neurosci 1999, 19:928-939.
12. Dhawan G., Floden A.M., Combs C.K. Amyloid-beta oligomers stimulate microglia through a tyrosine kinase dependent mechanism. Neurobiol Aging 2012, 33:2247-2261.
13. Fitzgerald K.A., Rowe D.C., Golenbock D.T. Endotoxin recognition and signal transduction by the TLR4/MD2-complex. Microbes Infect 2004, 6:1361-1367.
14. Floden A.M., Combs C.K. Beta-amyloid stimulates murine postnatal and adult microglia cultures in a unique manner. J Neurosci 2006, 26:4644-4648.
15. Floden A.M., Li S., Combs C.K. Beta-amyloid-stimulated microglia induce neuron death via synergistic stimulation of tumor necrosis factor alpha and NMDA receptors. J Neurosci 2005, 25:2566-2575.
16. Ganter S., Northoff H., Mannel D., Gebicke-Harter P.J. Growth control of cultured microglia. J Neurosci Res 1992, 33:218-230.
17. Gebicke-Haerter P.J., Bauer J., Schobert A., Northoff H. Lipopolysaccharide-free conditions in primary astrocyte cultures allow growth and isolation of microglial cells. J Neurosci 1989, 9:183-194.
18. Giulian D., Baker T.J. Characterization of ameboid microglia isolated from developing mammalian brain. J Neurosci 1986, 6:2163-2178.
19. Han I.O., Kim K.W., Ryu J.H., Kim W.K. p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-gamma, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells. Neurosci Lett 2002, 325:9-12.
20. Han L., Yin K., Zhang S., Wu Z., Wang C., Zhang Q., et al. Dalesconols B inhibits lipopolysaccharide induced inflammation and suppresses NF-kappaB and p38/JNK activation in microglial cells. Neurochem Int 2013, 62:913-921.
21. Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M. The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation. Altex 2009, 26:83-94.
22. Horvath R.J., Nutile-McMenemy N., Alkaitis M.S., Deleo J.A. Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures. J Neurochem 2008, 107:557-569.
23. Huysentruyt L.C., Akgoc Z., Seyfried T.N. Hypothesis: are neoplastic macrophages/microglia present in glioblastoma multiforme?. ASN Neuro 2011, 3:183-193.
24. Jordan F.L., Thomas W.E. Identification of microglia in primary cultures of mixed cerebral cortical cells. Brain Res Bull 1987, 19:153-159.
25. Kakimura J.-I., Kitamura Y., Takata K., Umeki M., Suzuki S., Shibagaki K., et al. Microglial activation and amyloid-β clearance induced by exogenous heat-shock proteins. FASEB J 2002, 16:601-603.
26. Kanzawa T., Sawada M., Kato K., Yamamoto K., Mori H., Tanaka R. Differentiated regulation of allo-antigen presentation by different types of murine microglial cell lines. J Neurosci Res 2000, 62:383-388.
27. Kopec K.K., Carroll R.T. Alzheimer's beta-amyloid peptide 1-42 induces a phagocytic response in murine microglia. J Neurochem 1998, 71:2123-2131.
28. Lee S.C., Liu W., Dickson D.W., Brosnan C.F., Berman J.W. Cytokine production by human fetal microglia and astrocytes. Differential induction by lipopolysaccharide and IL-1 beta. J Immunol 1993, 150:2659-2667.
29. Liu D., Wang Z., Liu S., Wang F., Zhao S., Hao A. Anti-inflammatory effects of fluoxetine in lipopolysaccharide (LPS)-stimulated microglial cells. Neuropharmacology 2011, 61:592-599.
30. McDonald D.R., Brunden K.R., Landreth G.E. Amyloid fibrils activate tyrosine kinase-dependent signaling and superoxide production in microglia. J Neurosci 1997, 17:2284-2294.
31. McGeer P.L., McGeer E.G. The inflammatory response system of brain: implications for therapy of Alzheimer and other neurodegenerative diseases. Brain Res Brain Res Rev 1995, 21:195-218.
32. Michelucci A., Heurtaux T., Grandbarbe L., Morga E., Heuschling P. Characterization of the microglial phenotype under specific pro-inflammatory and anti-inflammatory conditions: effects of oligomeric and fibrillar amyloid-beta. J Neuroimmunol 2009, 210:3-12.
33. Nagai A., Nakagawa E., Hatori K., Choi H.B., McLarnon J.G., Lee M.A., et al. Generation and characterization of immortalized human microglial cell lines: expression of cytokines and chemokines. Neurobiol Dis 2001, 8:1057-1068.
34. Nagamoto-Combs K., Combs C.K. Microglial phenotype is regulated by activity of the transcription factor, NFAT (nuclear factor of activated T cells). J Neurosci 2010, 30:9641-9646.
35. Nakamura Y., Si Q.S., Kataoka K. Lipopolysaccharide-induced microglial activation in culture: temporal profiles of morphological change and release of cytokines and nitric oxide. Neurosci Res 1999, 35:95-100.
36. Ohsawa K., Imai Y., Nakajima K., Kohsaka S. Generation and characterization of a microglial cell line, MG5, derived from a p53-deficient mouse. Glia 1997, 21:285-298.
37. Peudenier S., Héry C., Ng K.H., Tardieu M. HIV receptors within the brain: a study of CD4 and MHC-II on human neurons, astrocytes and microglial cells. Res Virol 1991, 142:145-149.
38. Pyo H., Jou I., Jung S., Hong S., Joe E.H. Mitogen-activated protein kinases activated by lipopolysaccharide and beta-amyloid in cultured rat microglia. Neuroreport 1998, 9:871-874.
39. Righi M., Mori L., De Libero G., Sironi M., Biondi A., Mantovani A., et al. Monokine production by microglial cell clones. Eur J Immunol 1989, 19:1443-1448.
40. Rojanathammanee L., Puig K.L., Combs C.K. Pomegranate polyphenols and extract inhibit nuclear factor of activated T-cell activity and microglial activation in vitro and in a transgenic mouse model of Alzheimer disease. J Nutr 2013, 143:597-605.
41. Sondag C.M., Dhawan G., Combs C.K. Beta amyloid oligomers and fibrils stimulate differential activation of primary microglia. J Neuroinflamm 2009, 6:1.
42. Stansley B., Post J., Hensley K. A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease. J Neuroinflamm 2012, 9:115.
43. Takata K., Kitamura Y., Saeki M., Terada M., Kagitani S., Kitamura R., et al. Galantamine-induced amyloid-{beta} clearance mediated via stimulation of microglial nicotinic acetylcholine receptors. J Biol Chem 2010, 285:40180-40191.
44. Tanaka J., Toku K., Matsuda S., Sudo S., Fujita H., Sakanaka M., et al. Induction of resting microglia in culture medium devoid of glycine and serine. Glia 1998, 24:198-215.
45. Terazawa R., Akimoto N., Kato T., Itoh T., Fujita Y., Hamada N., et al. A kavalactone derivative inhibits lipopolysaccharide-stimulated iNOS induction and NO production through activation of Nrf2 signaling in BV2 microglial cells. Pharmacol Res 2013, 71:34-43.
46. Woster A.P., Combs C.K. Differential ability of a thiazolidinedione PPARgamma agonist to attenuate cytokine secretion in primary microglia and macrophage-like cells. J Neurochem 2007, 103:67-76.
47. Zhou X., Spittau B., Krieglstein K. TGFbeta signalling plays an important role in IL4-induced alternative activation of microglia. J Neuroinflamm 2012, 9:210.
48. Zielasek J., Tausch M., Toyka K.V., Hartung H.P. Production of nitrite by neonatal rat microglial cells/brain macrophages. Cell Immunol 1992, 141:111-120.