Generation of transgenic pigs by cytoplasmic injection of piggyBac transposase-based pmGENIE-3 plasmids

Biology of Reproduction

Volumn 90, Issue 5, 2014, Pages

  Li, Zicong ^a   Zeng, Fang ^a   Meng, Fanming ^a   Xu, Zhiqian ^a   Zhang, Xianwei ^a   Huang, Xiaoling ^a   Tang, Fei ^a   Gao, Wenchao ^a   Shi, Junsong ^a   He, Xiaoyan ^a,b   Liu, Dewu ^a   Wang, Chong ^a   Urschitz, Johann ^c   Moisyadi, Stefan ^c,d   Wu, Zhenfang ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b Guangdong Wen's Research Institute   (China)

^c UNIVERSITY OF HAWAII   (United States)

^d Manoa BioSciences   (United States)

Author keywords

Pig; Piggybac; Pmgenie 3; Transgenesis; Transposase

Indexed keywords

GENOMIC DNA; PLASMID DNA; TRANSPOSASE; DNA;

AMPLICON; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOPLASM; EMBRYO; EMBRYO TRANSFER; FERTILIZATION IN VITRO; GENOME; INJECTION; INVERSE POLYMERASE CHAIN REACTION; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; OOCYTE; PIGLET; PLASMID; PORCINE EMBRYO; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENIC PIG; TRANSGENICS; ZYGOTE; ADMINISTRATION AND DOSAGE; ANIMAL; CHEMISTRY; FEMALE; GENE TRANSFER; GENE VECTOR; GENETICS; MALE; MICROINJECTION; NEWBORN; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; SURGERY; SWINE; TRANSGENIC ANIMAL; VETERINARY;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANIMALS, NEWBORN; BLOTTING, SOUTHERN; DNA; EMBRYO TRANSFER; FEMALE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; MALE; MICROINJECTIONS; PLASMIDS; POLYMERASE CHAIN REACTION; SWINE; TRANSGENES; TRANSPOSASES; ZYGOTE;

EID: 84903770205     PISSN: 00063363     EISSN: 15297268     Source Type: Journal    
DOI: 10.1095/biolreprod.113.116905     Document Type: Article

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