Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Simeonov, Kamen P ^a   Uppal, Hirdesh ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ALPHA FETOPROTEIN; HEPATOCYTE NUCLEAR FACTOR 1; HEPATOCYTE NUCLEAR FACTOR 1BETA; HEPATOCYTE NUCLEAR FACTOR 3ALPHA; HEPATOCYTE NUCLEAR FACTOR 3BETA; HEPATOCYTE NUCLEAR FACTOR 3GAMMA; HEPATOCYTE NUCLEAR FACTOR 4ALPHA; MESSENGER RNA; MICRORNA 122; MICRORNA 145; MICRORNA 192; MICRORNA 194; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR GATA 4; TRANSCRIPTION FACTOR GATA 6; TRANSCRIPTOME; UNCLASSIFIED DRUG; HEPATOCYTE NUCLEAR FACTOR 1ALPHA; HNF1A PROTEIN, HUMAN;

ADULT; AFP GENE; ALB GENE; ARTICLE; CEBPA GENE; CELL DIFFERENTIATION; CELL STRUCTURE; CONTROLLED STUDY; DIRECT REPROGRAMMING; DOWN REGULATION; FEMALE; FIBROBLAST; FOXA1 GENE; FOXA2 GENE; GATA4 GENE; GATA6 GENE; GENE; GENE ACTIVATION; GENE EXPRESSION; GENE INDUCTION; GENE LOCATION; GENETIC TRANSFECTION; HEPATOCYTE LIKE CELL; HHEX GENE; HNF1A GENE; HNF1B GENE; HNF4A GENE; HNF6A GENE; HUMAN; HUMAN CELL; LIVER CELL; MESSENGER RNA SYNTHESIS; NUCLEOTIDE SEQUENCE; PROCEDURES CONCERNING CELLS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RNA SEQUENCE; UPREGULATION; ANIMAL; CELL LINE; CYTOLOGY; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; MOUSE; NEWBORN; NUCLEAR REPROGRAMMING;

ADULT; ANIMALS; CELL LINE; CELLULAR REPROGRAMMING; FEMALE; FIBROBLASTS; GENE EXPRESSION PROFILING; HEPATOCYTE NUCLEAR FACTOR 1-ALPHA; HEPATOCYTES; HUMANS; INFANT, NEWBORN; MICE; RNA, MESSENGER;

EID: 84903542476     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0100134     Document Type: Article

References (30)

1. Chambers SM, Studer L (2011) Cell fate plug and play: direct reprogramming and induced pluripotency. Cell 145: 827-30.
2. Dhawan A, Puppi J, Hughes RD, Mitry RR (2010) Human hepatocyte transplantation: current experience and future challenges. Nat Rev Gastroenterol Hepatol 7: 288-98.
3. Kaplowitz N (2005) Idiosyncratic drug hepatotoxicity. Nat Rev Drug Discov 4: 489-99.
4. Thuluvath PJ, Guidinger MK, Fung JJ, Johnson LB, Rayhill SC, et al. (2010) Liver transplantation in the united states, 1999-2008. Am J Transplant 10: 1003-19.
5. Ben-David U, Benvenisty N (2011) The tumorigenicity of human embryonic and induced pluripotent stem cells. Nat Rev Cancer 11: 268-77.
6. Vierbuchen T, Wernig M (2011) Direct lineage conversions: unnatural but useful? Nat Biotechnol 29: 892-907.
7. Qian L, Huang Y, Spencer CI, Foley A, Vedantham V, et al. (2012) In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature 485: 593-8.
8. Song K, Nam YJ, Luo X, Qi X, Tan W, et al. (2012) Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 485: 599-604.
9. Huang P, He Z, Ji S, Sun H, Xiang D, et al. (2011) Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors. Nature 475: 386-9.
10. Sekiya S, Suzuki A (2011) Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475: 390-3.
11. Yu B, He ZY, You P, Han QW, Xiang D, et al. (2013) Reprogramming fibroblasts into bipotential hepatic stem cells by defined factors. Cell Stem Cell 13: 328-40.
12. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, et al. (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463: 1035-41.
13. Ieda M, Fu JD, Delgado-Olguin P, Vedantham V, Hayashi Y, et al. (2010) Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142: 375-86.
14. Bushman F, Lewinski M, Ciuffi A, Barr S, Leipzig J, et al. (2005) Genome-wide analysis of retroviral dna integration. Nat Rev Microbiol 3: 848-58. (Pubitemid 41693180)
15. Cirillo LA, Lin FR, Cuesta I, Friedman D, Jarnik M, et al. (2002) Opening of compacted chromatin by early developmental transcription factors hnf3 (foxa) and gata-4. Mol Cell 9: 279-89. (Pubitemid 34195554)
16. Lee CS, Friedman JR, Fulmer JT, Kaestner KH (2005) The initiation of liver development is dependent on foxa transcription factors. Nature 435: 944-7. (Pubitemid 40896319)
17. Odom DT, Dowell RD, Jacobsen ES, Nekludova L, Rolfe PA, et al. (2006) Core transcriptional regulatory circuitry in human hepatocytes. Mol Syst Biol 2: 2006-0017.
18. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mrna. Cell Stem Cell 7: 618-30.
19. Jang YY, Collector MI, Baylin SB, Diehl AM, Sharkis SJ (2004) Hematopoietic stem cells convert into liver cells within days without fusion. Nat Cell Biol 6: 532-9. (Pubitemid 38786596)
20. Theise ND, Nimmakayalu M, Gardner R, Illei PB, Morgan G, et al. (2000) Liver from bone marrow in humans. Hepatology 32: 11-6. (Pubitemid 30432360)
21. Phuc Le P, Friedman JR, Schug J, Brestelli JE, Parker JB, et al. (2005) Glucocorticoid receptor-dependent gene regulatory networks. PLoS Genet 1: e16.
22. Lee J, Sayed N, Hunter A, Au KF, Wong WH, et al. (2012) Activation of innate immunity is required for efficient nuclear reprogramming. Cell 151: 547-58.
23. Stainier DY (2002) A glimpse into the molecular entrails of endoderm formation. Genes Dev 16: 893-907.
24. Ohtake Y, Maruko A, Ohishi N, Kawaguchi M, Satoh T, et al. (2008) Effect of retinoic acid on transglutaminase and ornithine decarboxylase activities during liver regeneration. Cell Biochem Funct 26: 359-65. (Pubitemid 351613178)
25. Wasmuth HE, Lammert F, Zaldivar MM, Weiskirchen R, Hellerbrand C, et al. (2009) Antifibrotic effects of cxcl9 and its receptor cxcr3 in livers of mice and humans. Gastroenterology 137: 309-19, 319 e1-3.
26. Girard M, Jacquemin E, Munnich A, Lyonnet S, Henrion-Caude A (2008) mir-122, a paradigm for the role of micrornas in the liver. J Hepatol 48: 648-56.
27. Liu X, Yu X, Zack DJ, Zhu H, Qian J (2008) Tiger: a database for tissue-specific gene expression and regulation. BMC Bioinformatics 9: 271.
28. Pang ZP, Yang N, Vierbuchen T, Ostermeier A, Fuentes DR, et al. (2011) Induction of human neuronal cells by defined transcription factors. Nature 476: 220-3.
29. Du Y, Wang J, Jia J, Song N, Xiang C, et al. (2014) Human hepatocytes with drug metabolic function induced from fibroblasts by lineage reprogramming. Cell stem cell 14: 394-403.
30. Huang P, Zhang L, Gao Y, He Z, Yao D, et al. (2014) Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell stem cell 14: 370-84.