Modified-chitosan/siRNA nanoparticles downregulate cellular CDX2 expression and cross the gastric mucus barrier

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Sadio, Ana ^a,b,c,d,e   Gustafsson, Jenny K ^f   Pereira, Bruno ^a   Gomes, Carla Pereira ^a,b   Hansson, Gunnar C ^f   David, Leonor ^a,e   Pêgo, Ana Paula ^a,b   Almeida, Raquel ^a,e  

^a UNIVERSITY OF PORTO   (Portugal)

^b INEB INSTITUTO DE ENGENHARIA BIOMÉDICA   (Portugal)

^c Serviço de Ortopedia   (Portugal)

^d Gulbenkian Program for Advanced Medical Education   (Portugal)

^e University of Porto   (Portugal)

^f UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHITOSAN; NANOPARTICLE; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR CDX2; CDX2 PROTEIN, HUMAN; HOMEODOMAIN PROTEIN; IMIDAZOLE; IMIDAZOLE DERIVATIVE;

ACIDITY; ARTICLE; CARCINOMA CELL; CELL VIABILITY; DOWN REGULATION; EPITHELIUM CELL; HYDROPHOBICITY; PARTICLE SIZE; PH; PROTEIN EXPRESSION; PROTEIN TARGETING; STOMACH CARCINOMA; STOMACH MUCUS; ZETA POTENTIAL; ANIMAL; CHEMISTRY; COLON; GENETIC TRANSFECTION; HUMAN; METABOLISM; MOUSE; MUCUS; STOMACH; TUMOR CELL LINE; ULTRASTRUCTURE;

ANIMALS; CELL LINE, TUMOR; CHITOSAN; COLON; DOWN-REGULATION; HOMEODOMAIN PROTEINS; HUMANS; IMIDAZOLES; MICE; MUCUS; NANOPARTICLES; RNA, SMALL INTERFERING; STOMACH; TRANSFECTION;

EID: 84903390267     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0099449     Document Type: Article

References (31)

1. Luo J, Solimini NL, Elledge SJ (2009) Principles of cancer therapy: oncogene and non-oncogene addiction. Cell 136: 823-837.
2. Vias M, Ramos-Montoya A, Mills IG (2008) Terminal and progenitor lineagesurvival oncogenes as cancer markers. Trends Mol Med 14: 486-494.
3. Miele E, Spinelli GP, Miele E, Di Fabrizio E, Ferretti E, et al. (2012) Nanoparticle-based delivery of small interfering RNA: challenges for cancer therapy. Int J Nanomedicine 7: 3637-3657.
4. Fire A, Xu SQ, Montgomery MK, Kostas SA, Driver SE, et al. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811. (Pubitemid 28099681)
5. Elbashir SM, Lendeckel W, Tuschl T (2001) RNA interference is mediated by 21-and 22-nucleotide RNAs. Genes & Development 15: 188-200. (Pubitemid 32107664)
6. Gavrilov K, Saltzman WM (2012) Therapeutic siRNA: principles, challenges, and strategies. Yale J Biol Med 85: 187-200.
7. Howard KA, Rahbek UL, Liu X, Damgaard CK, Glud SZ, et al. (2006) RNA interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle system. Mol Ther 14: 476-484. (Pubitemid 44296092)
8. Mao S, Sun W, Kissel T (2010) Chitosan-based formulations for delivery of DNA and siRNA. Advanced Drug Delivery Reviews 62: 12-27.
9. Mittnacht U, Hartmann H, Hein S, Oliveira H, Dong M, et al. (2010) Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth. Nano Lett 10: 3933-3939.
10. Moreira C, Oliveira H, Pires LR, Simões S, Barbosa MA, et al. (2009) Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone. Acta Biomaterialia 5: 2995-3006.
11. Kotze AF, Luessen HL, de Leeuw BJ, de Boer BG, Verhoef JC, et al. (1997) N-trimethyl chitosan chloride as a potential absorption enhancer across mucosal surfaces: in vitro evaluation in intestinal epithelial cells (Caco-2). Pharm Res 14: 1197-1202. (Pubitemid 27432543)
12. Barros R, Freund JN, David L, Almeida R (2012) Gastric intestinal metaplasia revisited: function and regulation of CDX2. Trends Mol Med 18: 555-563.
13. Barros R, Camilo V, Pereira B, Freund JN, David L, et al. (2010) Pathophysiology of intestinal metaplasia of the stomach: emphasis on CDX2 regulation. Biochem Soc Trans 38: 358-363.
14. Barros R, da Costa LT, Pinto-de-Sousa J, Duluc I, Freund JN, et al. (2011) CDX2 autoregulation in human intestinal metaplasia of the stomach: impact on the stability of the phenotype. Gut 60: 290-298.
15. Mallo GV, Soubeyran P, Lissitzky JC, André F, Farnarier C, et al. (1998) Expression of the Cdx1 and Cdx2 homeotic genes leads to reduced malignancy in colon cancer-derived cells. J Biol Chem 273: 14030-14036. (Pubitemid 28268460)
16. Bonhomme C, Duluc I, Martin E, Chawengsaksophak K, Chenard MP, et al. (2003) The Cdx2 homeobox gene has a tumour suppressor function in the distal colon in addition to a homeotic role during gut development. Gut 52: 1465-1471. (Pubitemid 37176727)
17. Salari K, Spulak ME, Cuff J, Forster AD, Giacomini CP, et al. (2012) CDX2 is an amplified lineage-survival oncogene in colorectal cancer. Proc Natl Acad Sci U S A 109: E3196-3205.
18. Breunig M, Lungwitz U, Liebl R, Goepferich A (2007) Breaking up the correlation between efficacy and toxicity for nonviral gene delivery. Proc Natl Acad Sci U S A 104: 14454-14459. (Pubitemid 350003282)
19. Ensign LM, Cone R, Hanes J (2012) Oral drug delivery with polymeric nanoparticles: the gastrointestinal mucus barriers. Adv Drug Deliv Rev 64: 557-570.
20. Sandri G, Bonferoni MC, Rossi S, Ferrari F, Boselli C, et al. (2010) Insulinloaded nanoparticles based on N-trimethyl chitosan: in vitro (Caco-2 model) and ex vivo (excised rat jejunum, duodenum, and ileum) evaluation of penetration enhancement properties. AAPS PharmSciTech 11: 362-371.
21. Lai SK, Wang YY, Hanes J (2009) Mucus-penetrating nanoparticles for drug and gene delivery to mucosal tissues. Adv Drug Deliv Rev 61: 158-171.
22. Atuma C, Strugala V, Allen A, Holm L (2001) The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo. Am J Physiol Gastrointest Liver Physiol 280: G922-929.
23. Johansson ME, Phillipson M, Petersson J, Velcich A, Holm L, et al. (2008) The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Proc Natl Acad Sci U S A 105: 15064-15069.
24. Johansson ME, Sjovall H, Hansson GC (2013) The gastrointestinal mucus system in health and disease. Nat Rev Gastroenterol Hepatol 10: 352-361.
25. Gustafsson JK, Ermund A, Johansson ME, Schutte A, Hansson GC, et al. (2012) An ex vivo method for studying mucus formation, properties, and thickness in human colonic biopsies and mouse small and large intestinal explants. Am J Physiol Gastrointest Liver Physiol 302: G430-438.
26. Ballarín-González B, Dagnaes-Hansen F, Fenton RA, Gao S, Hein S, et al. (2013) Protection and Systemic Translocation of siRNA Following Oral Administration of Chitosan/siRNA Nanoparticles. Mol Ther Nucleic Acids doi: 10.1038/mtna.2013.2
27. Caldara M, Friedlander RS, Kavanaugh NL, Aizenberg J, Foster KR, et al. (2012) Mucin biopolymers prevent bacterial aggregation by retaining cells in the free-swimming state. Curr Biol 22: 2325-2330.
28. Lai SK, O'Hanlon DE, Harrold S, Man ST, Wang YY, et al. (2007) Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus. Proc Natl Acad Sci U S A 104: 1482-1487. (Pubitemid 46214616)
29. Mourya VK, Inamdar NN (2009) Trimethyl chitosan and its applications in drug delivery. J Mater Sci Mater Med 20: 1057-1079.
30. Gartner F, David L, Seruca R, Machado JC, Sobrinho-Simoes M (1996) Establishment and characterization of two cell lines derived from human diffuse gastric carcinomas xenografted in nude mice. Virchows Arch 428: 91-98. (Pubitemid 26177974)
31. Alameh M, Jean M, Dejesus D, Buschmann MD, Merzouki A (2010) Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing. Int J Nanomedicine 5: 473-481.