Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing

International Journal of Nanomedicine

Volumn 5, Issue 1, 2010, Pages 473-481

  Alameh, Mohamad ^a   Jean, Myriam ^a   Dejesus, Diogo ^a   Buschmann, Michael D ^a   Merzouki, Abderrazzak ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

Chitosan; Chitosanase; DPP IV gene silencing; qPCR; RIN; siRNA

Indexed keywords

CHITOSAN; CHITOSANASE; MESSENGER RNA; NANOPARTICLE; SMALL INTERFERING RNA; GLYCOSIDASE; RNA;

ARTICLE; CELL LYSATE; CELL TRANSPORT; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; MOLECULAR WEIGHT; PRODUCT RECOVERY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; RNA PURIFICATION; RNA SYNTHESIS; STREPTOMYCES GRISEUS; CELL STRAIN CACO 2; CELL STRAIN HEPG2; CELL STRAIN HT29; CHEMISTRY; DRUG DELIVERY SYSTEM; GENETICS; HUMAN; IN VITRO STUDY; ISOLATION AND PURIFICATION; NANOMEDICINE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CACO-2 CELLS; CHITOSAN; DRUG DELIVERY SYSTEMS; GENE SILENCING; GLYCOSIDE HYDROLASES; HEP G2 CELLS; HT29 CELLS; HUMANS; NANOMEDICINE; NANOPARTICLES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 79952114990     PISSN: 11769114     EISSN: 11782013     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Illum L. Chitosan and its use as a pharmaceutical excipient. Pharm Res. 1998;15:1326-1331.
2. Huang M, Khor E, Lim LY. Uptake and cytotoxicity of chitosan molecules and nanoparticles: effects of molecular weight and degree of deacetylation. Pharm Res. 2004;21:344-353.
3. Zhang H, Neau SH. In vitro degradation of chitosan by a commercial enzyme preparation: effect of molecular weight and degree of deacetylation. Biomaterials. 2001;22:1653-1658.
4. Prego C, Torres D, Alonso MJ. Chitosan nanocapsules as carriers for oral peptide delivery: effect of chitosan molecular weight and type of salt on the in vitro behaviour and in vivo effectiveness. J Nanosci Nanotechnol. 2006;6:2921-2928.
5. Prego C, Fabre M, Torres D, Alonso MJ. Efficacy and mechanism of action of chitosan nanocapsules for oral peptide delivery. Pharm Res. 2006;23:549-556.
6. Prego C, Torres D, Fernandez-Megia E, Novoa-Carballal R, Quinoa E, Alonso MJ. Chitosan-PEG nanocapsules as new carriers for oral peptide delivery. Effect of chitosan pegylation degree. J Control Release. 2006;111:299-308.
7. Jean M, Smaoui F, Lavertu M, et al. Chitosan-plasmid nanoparticle formulations for IM and SC delivery of recombinant FGF-2 and PDGF-BB or generation of antibodies. Gene Ther. 2009;16:1097-1110.
8. Centelles MN, Isasi JR, Qian C, Campanero MA, Irache JM. Influence of the chitosan nature on the transfection efficacy of DNA-loaded nanoparticles after hydrodynamic administration in mice. J Microencapsul. Feb 15 2010; [Epub ahead of print].
9. Zheng F, Shi XW, Yang GF, et al. Chitosan nanoparticle as gene therapy vector via gastrointestinal mucosa administration: results of an in vitro and in vivo study. Life Sci. 2007;80:388-396.
10. Zheng Y, Yang W, Wang C, et al. Nanoparticles based on the complex of chitosan and polyaspartic acid sodium salt: preparation, characterization and the use for 5-fluorouracil delivery. Eur J Pharm Biopharm. 2007;67:621-631.
11. Lavertu M, Methot S, Tran-Khanh N, Buschmann MD. High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials. 2006;27:4815-4824.
12. Howard KA, Rahbek UL, Liu X, et al. RNA interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle system. Mol Ther. 2006;14:476-484.
13. Futami K, Kumagai E, Makino H, et al. Anticancer activity of RecQL1 helicase siRNA in mouse xenograft models. Cancer Sci. 2008;99:1227-1236.
14. Gao S, Dagnaes-Hansen F, Nielsen EJ, et al. The effect of chemical modification and nanoparticle formulation on stability and biodistribution of siRNA in mice. Mol Ther. 2009;17:1225-1233.
15. Bustin SA. Quantif ication of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol. 2002;29:23-39.
16. Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol. 2002;30:503-512.
17. Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW. Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett. 2006;28:1601-1613.
18. Fleige S, Pfaffl MW. RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med. 2006;27:126-139.
19. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:e45.
20. Arocho A, Chen B, Ladanyi M, Pan Q. Validation of the 2-ΔΔCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagn Mol Pathol. 2006;15: 56-61.
21. Tchemtchoua VT, Atanasova G, Aqil A, et al. Development of a procedure to simultaneously isolate RNA, DNA, and proteins for [corrected] characterizing cells invading or cultured on chitosan scaffolds. Anal Biochem. 2009;393:145-147.
22. Gambino G, Perrone I, Gribaudo I. A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochem Anal. 2008;19:520-525.
23. Skrypina NA, Timofeeva AV, Khaspekov GL, Savochkina LP, Beabealashvilli R. Total RNA suitable for molecular biology analysis. J Biotechnol. 2003;105:1-9.
24. MacRae E. Extraction of plant RNA. Methods Mol Biol. 2007;353: 15-24.
25. George M, Abraham TE. Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan - a review. J Control Release. 2006;114:1-14.
26. Qiagen, ed. RNeasy mini handbook. 4th ed; 2004.
27. Hoemann CD, Sun J, Chrzanowski V, Buschmann MD. A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage. Anal Biochem. 2002;300:1-10.
28. Ma PL, Lavertu M, Winnik FM, Buschmann MD. New insights into chitosan-DNA interactions using isothermal titration microcalorimetry. Biomacromolecules. 2009;10:1490-1499.
29. Tanabe T, Morinaga K, Fukamizo T, Mitsutomi M. Novel chitosanase from Streptomyces griseus HUT 6037 with transglycosylation activity. Biosci Biotechnol Biochem. 2003;67:354-364.
30. Jung HS, Son JW, Ji HS, Kim K. Effective Production of Chitinase and Chitosanase by Streptomyces griseus HUT 6037 Using Colloidal Chitin and Various Degrees of Deacetylation of Chitosan. Biotechnot Bioprocess Eng. 1999;4:26-31.
31. Mason PE, Neilson GW, Dempsey CE, Barnes AC, Cruickshank JM. The hydration structure of guanidinium and thiocyanate ions: implications for protein stability in aqueous solution. Proc Natl Acad Sci U S A. 2003;100:4557-4561.
32. Cockle SA, Epand RM, Moscarello MA. Resistance of lipophilin, a hydrophobic myelin protein, to denaturation by urea and guanidinium salts. J Biol Chem. 1978;253:8019-8026.
33. Ekowati C, Hariyadi P, Witarto AB, Hwang JK, Suhartono MT. Biochemical characteristics of chitosanase from the Indonesian Bacillus licheniformis MB-2. Mol Biotechnol. 2006;33:93-102.
34. Yoon HG, Kim HY, Lim YH, et al. Thermostable chitosanase from Bacillus sp. Strain CK4: cloning and expression of the gene and characterization of the enzyme. Appl Environ Microbiol. 2000;66:3727-3734.
35. Mao HQ, Roy K, Troung-Le VL, et al. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J Control Release. 2001;70:399-421.