High-throughput assays for superoxide and hydrogen peroxide design of a screening workflow to identify inhibitors of NADPH oxidases

Journal of Biological Chemistry

Volumn 289, Issue 23, 2014, Pages 16176-16189

  Zielonka, Jacek ^a   Cheng, Gang ^a   Zielonka, Monika ^a   Ganesh, Thota ^b   Sun, Aiming ^c   Joseph, Joy ^a   Michalski, Radosław ^a   O'Brien, William J ^c   Lambeth, J David ^d   Kalyanaraman, Balaraman ^a  

^a FREE RADICAL RESEARCH CENTER   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d EMORY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME INHIBITION; OXYGEN; PEROXIDES;

CELLULAR PRODUCTION; CONCEPTUAL UNDERSTANDING; FLUORESCENCE MEASUREMENTS; HIGH-THROUGHPUT ASSAYS; REACTIVE OXYGEN SPECIES; SELECTIVE INHIBITORS; SIMULTANEOUS MONITORING; SUPEROXIDE RADICAL ANIONS;

HYDROGEN PEROXIDE;

2 HYDROXYETHIDIUM; 2 HYDROXYPROPIDIUM; AMPLEX RED; APOCYNIN; DIAPOCYNIN; EBSELEN; HYDROETHIDINE; HYDROGEN PEROXIDE; HYDROPROPIDINE; OXIDOPAMINE; OXIDOREDUCTASE INHIBITOR; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE 2; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE 4; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE INHIBITOR; RESORUFIN; SUPEROXIDE; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; VAS 2870;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MOLECULAR PROBE; OXIDATION KINETICS; OXIMETRY; PRIORITY JOURNAL; SPIN TRAPPING; STRUCTURE ANALYSIS;

ENZYME INHIBITOR; FLUORESCENCE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); HIGH-THROUGHPUT SCREENING (HTS); HYDROGEN PEROXIDE; NADPH OXIDASE; REACTIVE OXYGEN SPECIES (ROS); SUPEROXIDE ION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HL-60 CELLS; HUMANS; HYDROGEN PEROXIDE; MASS SPECTROMETRY; NADPH OXIDASE; OXIDATION-REDUCTION; REACTIVE OXYGEN SPECIES; SPIN LABELS; SUPEROXIDES;

EID: 84902206908     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.548693     Document Type: Article

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